All Outputs (29)

Human gnathostomiasis: a neglected food-borne zoonosis (2020)
Journal Article
Liu, G., Sun, M., Elsheikha, H. M., Fu, Y., Sugiyama, H., Ando, K., …Yao, C. (2020). Human gnathostomiasis: a neglected food-borne zoonosis. Parasites and Vectors, 13, Article 616. https://doi.org/10.1186/s13071-020-04494-4

Background: Human gnathostomiasis is a food-borne zoonosis. Its etiological agents are the third-stage larvae of Gnathostoma spp. Human gnathostomiasis is often reported in developing countries, but it is also an emerging disease in developed countri... Read More about Human gnathostomiasis: a neglected food-borne zoonosis.

Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica (2020)
Journal Article
Shi, W., He, J., Mei, X., Lu, K., Zeng, Z., Zhang, Y., …Zhu, X. (2020). Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica. PLoS Neglected Tropical Diseases, 14(12), Article e0008951. https://doi.org/10.1371/journal.pntd.0008951

The excretory-secretory products released by the liver fluke Fasciola gigantica (FgESPs) play important roles in regulating the host immune response during the infection. Identification of hepatic miRNAs altered by FgESPs may improve our understandin... Read More about Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica.

ROP18-Mediated Transcriptional Reprogramming of HEK293T Cell Reveals New Roles of ROP18 in the Interplay Between Toxoplasma gondii and the Host Cell (2020)
Journal Article
Li, J., He, J., Elsheikha, H. M., Ma, J., Xu, X., & Zhu, X. (2020). ROP18-Mediated Transcriptional Reprogramming of HEK293T Cell Reveals New Roles of ROP18 in the Interplay Between Toxoplasma gondii and the Host Cell. Frontiers in Cellular and Infection Microbiology, 10, Article 586946. https://doi.org/10.3389/fcimb.2020.586946

Toxoplasma gondii secretes a number of virulence-related effector proteins, such as the rhoptry protein 18 (ROP18). To further broaden our understanding of the molecular functions of ROP18, we examined the transcriptional response of human embryonic... Read More about ROP18-Mediated Transcriptional Reprogramming of HEK293T Cell Reveals New Roles of ROP18 in the Interplay Between Toxoplasma gondii and the Host Cell.

Molecular prevalence, risk factors and genotypes of Toxoplasma gondii DNA in wild marine snails collected from offshore waters in eastern China (2020)
Journal Article
Cong, W., Elsheikha, H. M., Li, M., Ma, J., Zou, Y., & Jiang, Z. (2021). Molecular prevalence, risk factors and genotypes of Toxoplasma gondii DNA in wild marine snails collected from offshore waters in eastern China. Acta Tropica, 214, https://doi.org/10.1016/j.actatropica.2020.105779

Increasing evidence exisits for the role that shellfish play in the epidemiology of Toxoplasma gondii in marine environment. However, limited information is available on the level of T. gondii infection in wild marine snails, which can play a role in... Read More about Molecular prevalence, risk factors and genotypes of Toxoplasma gondii DNA in wild marine snails collected from offshore waters in eastern China.

Epidemiology, pathophysiology, diagnosis, and management of cerebral toxoplasmosis (2020)
Journal Article
Elsheikha, H. M., Marra, C. M., & Zhu, X. (2020). Epidemiology, pathophysiology, diagnosis, and management of cerebral toxoplasmosis. Clinical Microbiology Reviews, 34(1), https://doi.org/10.1128/CMR.00115-19

Toxoplasma gondii is known to infect a considerable number of mammalian and avian species and a substantial proportion of the world’s human population. The parasite has an impressive ability to disseminate within the host’s body and employs various t... Read More about Epidemiology, pathophysiology, diagnosis, and management of cerebral toxoplasmosis.

Proteomic profiling of the liver, hepatic lymph nodes, and spleen of buffaloes infected with fasciola gigantica (2020)
Journal Article
Hu, R., Zhang, F., Elsheikha, H. M., Ma, Q., Ehsan, M., Zhao, Q., & Zhu, X. (2020). Proteomic profiling of the liver, hepatic lymph nodes, and spleen of buffaloes infected with fasciola gigantica. Pathogens, 9(12), Article 982. https://doi.org/10.3390/pathogens9120982

In the present study, we used an isobaric tag for relative and absolute quantitation (iTRAQ) proteomics technology to characterize the differentially expressed proteins (DEPs) in the liver, hepatic lymph nodes (hLNs), and spleen of buffaloes infected... Read More about Proteomic profiling of the liver, hepatic lymph nodes, and spleen of buffaloes infected with fasciola gigantica.

Toxocara canis Differentially Affects Hepatic MicroRNA Expression in Beagle Dogs at Different Stages of Infection (2020)
Journal Article
Zou, Y., Zheng, W., He, J., Elsheikha, H. M., Zhu, X., & Lu, Y. (2020). Toxocara canis Differentially Affects Hepatic MicroRNA Expression in Beagle Dogs at Different Stages of Infection. Frontiers in Veterinary Science, 7, https://doi.org/10.3389/fvets.2020.587273

Toxocara canis is a neglected zoonotic parasite, which threatens the health of dogs and humans worldwide. The molecular mechanisms that underlie the progression of T. canis infection remain mostly unknown. MicroRNAs (miRNAs) are small non-coding RNAs... Read More about Toxocara canis Differentially Affects Hepatic MicroRNA Expression in Beagle Dogs at Different Stages of Infection.

Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells (2020)
Journal Article
Pérez-Cota, F., Fuentes-Domínguez, R., La Cavera III, S., Hardiman, W., Yao, M., Setchfield, K., …Clark, M. (2020). Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells. Journal of Applied Physics, 128(16), Article 160902. https://doi.org/10.1063/5.0023744

© 2020 Author(s). Characterization of the elasticity of biological cells is growing as a new way to gain insight into cell biology. Cell mechanics are related to most aspects of cellular behavior, and applications in research and medicine are broad.... Read More about Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells.

Transcriptomic Profiling of Mouse Brain During Acute and Chronic Infections by Toxoplasma gondii Oocysts (2020)
Journal Article
Hu, R., He, J., Elsheikha, H. M., Zou, Y., Ehsan, M., Ma, Q., …Cong, W. (2020). Transcriptomic Profiling of Mouse Brain During Acute and Chronic Infections by Toxoplasma gondii Oocysts. Frontiers in Microbiology, 11, Article 570903. https://doi.org/10.3389/fmicb.2020.570903

Infection by the protozoan Toxoplasma gondii can have a devastating impact on the structure and function of the brain of the infected individuals, particularly immunocompromised patients. A systems biology view of the brain transcriptome can identify... Read More about Transcriptomic Profiling of Mouse Brain During Acute and Chronic Infections by Toxoplasma gondii Oocysts.

Global profiling of lysine 2-hydroxyisobutyrylome in Toxoplasma gondii using affinity purification mass spectrometry (2020)
Journal Article
Nie, L., Liang, Q., Elsheikha, H. M., Du, R., Zhu, X., & Li, F. (2020). Global profiling of lysine 2-hydroxyisobutyrylome in Toxoplasma gondii using affinity purification mass spectrometry. Parasitology Research, 119(12), 4061-4071. https://doi.org/10.1007/s00436-020-06923-w

Lysine 2-hydroxyisobutyrylation (Khib) is a recently discovered and evolutionarily conserved form of protein post-translational modification (PTM) found in mammalian and yeast cells. Previous studies have shown that Khib plays roles in the activity o... Read More about Global profiling of lysine 2-hydroxyisobutyrylome in Toxoplasma gondii using affinity purification mass spectrometry.

Global profiling of lncRNAs-miRNAs-mRNAs reveals differential expression of coding genes and non-coding RNAs in the lung of beagle dogs at different stages of Toxocara canis infection (2020)
Journal Article
Zheng, W., Zou, Y., He, J., Elsheikha, H. M., Liu, G., Hu, M., …Zhu, X. (2021). Global profiling of lncRNAs-miRNAs-mRNAs reveals differential expression of coding genes and non-coding RNAs in the lung of beagle dogs at different stages of Toxocara canis infection. International Journal for Parasitology, 51(1), 49-61. https://doi.org/10.1016/j.ijpara.2020.07.014

The roundworm Toxocara canis causes toxocariasis in dogs and larval migrans in humans. Better understanding of the lung response to T. canis infection could explain why T. canis must migrate to and undergoes part of its development inside the lung of... Read More about Global profiling of lncRNAs-miRNAs-mRNAs reveals differential expression of coding genes and non-coding RNAs in the lung of beagle dogs at different stages of Toxocara canis infection.

Acetylome analysis of the feline small intestine following Toxoplasma gondii infection (2020)
Journal Article
Meng, Y., Zhai, B., Elsheikha, H. M., Xie, S., Wang, Z., Zhao, Q., …He, J. (2020). Acetylome analysis of the feline small intestine following Toxoplasma gondii infection. Parasitology Research, 119(11), 3649-3657. https://doi.org/10.1007/s00436-020-06880-4

Toxoplasma gondii is a protozoan parasite capable of infecting a large number of warm-blooded animals and causes serious health complications in immunocompromised patients. T. gondii infection of the feline small intestine is critical for the complet... Read More about Acetylome analysis of the feline small intestine following Toxoplasma gondii infection.

In Vitro Growth- and Encystation-Inhibitory Efficacies of Matcha Green Tea and Epigallocatechin Gallate Against Acanthameoba Castellanii (2020)
Journal Article
Dickson, A., Cooper, E., Fakae, L. B., Wang, B., Chan, K. L. A., & Elsheikha, H. M. (2020). In Vitro Growth- and Encystation-Inhibitory Efficacies of Matcha Green Tea and Epigallocatechin Gallate Against Acanthameoba Castellanii. Pathogens, 9(9), Article 763. https://doi.org/10.3390/pathogens9090763

We examined the inhibitory effect of matcha green tea (Camellia sinensis) and epigallocatechin gallate (EGCg; the most abundant catechin in tea) on the vegetative growth and encystation of Acanthamoeba castellanii T4 genotype. The sulforhodamine B (S... Read More about In Vitro Growth- and Encystation-Inhibitory Efficacies of Matcha Green Tea and Epigallocatechin Gallate Against Acanthameoba Castellanii.

Nematocidal effects of a coriander essential oil and five pure principles on the infective larvae of major ovine gastrointestinal nematodes in vitro (2020)
Journal Article
Helal, M. A., Abdel-Gawad, A. M., Kandil, O. M., Khalifa, M. M. E., Cave, G. W. V., Morrison, A. A., …Elsheikha, H. M. (2020). Nematocidal effects of a coriander essential oil and five pure principles on the infective larvae of major ovine gastrointestinal nematodes in vitro. Pathogens, 9(9), 1-17. https://doi.org/10.3390/pathogens9090740

The anthelmintic effects of extracted coriander oil and five pure essential oil constituents (geraniol, geranyl acetate, eugenol, methyl iso-eugenol, and linalool) were tested, using larval motility assay, on the third-stage larvae (L3s) of Haemonchu... Read More about Nematocidal effects of a coriander essential oil and five pure principles on the infective larvae of major ovine gastrointestinal nematodes in vitro.

Structural, functional, and metabolic alterations in human cerebrovascular endothelial cells during toxoplasma gondii infection and amelioration by verapamil in vitro (2020)
Journal Article
Al-Sandaqchi, A. T., Marsh, V., Williams, H. E. L., Stevenson, C. W., & Elsheikha, H. M. (2020). Structural, functional, and metabolic alterations in human cerebrovascular endothelial cells during toxoplasma gondii infection and amelioration by verapamil in vitro. Microorganisms, 8(9), Article 1386. https://doi.org/10.3390/microorganisms8091386

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Toxoplasma gondii (T. gondii), the causative agent of toxoplasmosis, is a frequent cause of brain infection. Despite its known ability to invade the brain, there is still a dire need to better... Read More about Structural, functional, and metabolic alterations in human cerebrovascular endothelial cells during toxoplasma gondii infection and amelioration by verapamil in vitro.

Impact of neospora caninum infection on the bioenergetics and transcriptome of cerebrovascular endothelial cells (2020)
Journal Article
Elsheikha, H. M., Alkurashi, M., Palfreman, S., Castellanos, M., Kong, K., Ning, E., …MacNaughtan, W. (2020). Impact of neospora caninum infection on the bioenergetics and transcriptome of cerebrovascular endothelial cells. Pathogens, 9(9), Article 710. https://doi.org/10.3390/pathogens9090710

In this work, the effects of the protozoan Neospora caninum on the bioenergetics, chemical composition, and elemental content of human brain microvascular endothelial cells (hBMECs) were investigated. We showed that N. caninum can impair cell mitocho... Read More about Impact of neospora caninum infection on the bioenergetics and transcriptome of cerebrovascular endothelial cells.

Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro (2020)
Journal Article
Harun, M., Marsh, V., Elsaied, N. A., Webb, K. F., & Elsheikha, H. M. (2020). Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro. Brain Research, 1746, Article 147002. https://doi.org/10.1016/j.brainres.2020.147002

Toxoplasma gondii can cause parasitic encephalitis, a life-threatening infection that predominately occurs in immunocompromised individuals. T. gondii has the ability to invade the brain, but the mechanisms by which this parasite crosses the blood–br... Read More about Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro.

Transcriptome Profiling of Toxoplasma gondii-Infected Human Cerebromicrovascular Endothelial Cell Response to Treatment with Monensin (2020)
Journal Article
Elsheikha, H. M., Harun, M. S. R., Taylor, M., & Zhu, X. (2020). Transcriptome Profiling of Toxoplasma gondii-Infected Human Cerebromicrovascular Endothelial Cell Response to Treatment with Monensin. Microorganisms, 8(6), https://doi.org/10.3390/microorganisms8060842

Central to the progression of cerebral toxoplasmosis is the interaction of Toxoplasma gondii with the blood-brain barrier (BBB) endothelial cells. In the present work, we tested the hypothesis that inhibition of Wnt pathway signalling by the monovale... Read More about Transcriptome Profiling of Toxoplasma gondii-Infected Human Cerebromicrovascular Endothelial Cell Response to Treatment with Monensin.

First record of besnoitiosis caused by Besnoitia bennetti in donkeys from the UK (2020)
Journal Article
Elsheikha, H. M., Schares, G., Paraschou, G., Sullivan, R., & Fox, R. (2020). First record of besnoitiosis caused by Besnoitia bennetti in donkeys from the UK. Parasites and Vectors, 13, https://doi.org/10.1186/s13071-020-04145-8

Background: The involvement of Besnoitia bennetti in skin pathologies was investigated in a series of 20 donkeys from the Donkey Sanctuary in England, in the 2013-2019 period. Methods: The initial histopathological finding of Besnoitia cysts in skin... Read More about First record of besnoitiosis caused by Besnoitia bennetti in donkeys from the UK.

In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii (2020)
Journal Article
Fakae, L. B., Stevenson, C. W., Zhu, X. Q., & Elsheikha, H. M. (2020). In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii. International Journal for Parasitology: Drugs and Drug Resistance, 13, 59-72. https://doi.org/10.1016/j.ijpddr.2020.05.001

© 2020 The Author(s) The effect of Camellia sinensis (green tea) on the growth of Acanthamoeba castellanii trophozoites was examined using a microplate based-Sulforhodamine B (SRB) assay. C. sinensis hot and cold brews at 75% and 100% concentrations... Read More about In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii.