Skip to main content

Research Repository

Advanced Search

ROP18-Mediated Transcriptional Reprogramming of HEK293T Cell Reveals New Roles of ROP18 in the Interplay Between Toxoplasma gondii and the Host Cell

Li, Jie-Xi; He, Jun-Jun; Elsheikha, Hany M.; Ma, Jun; Xu, Xiao-Pei; Zhu, Xing-Quan

Authors

Jie-Xi Li

Jun-Jun He

Jun Ma

Xiao-Pei Xu

Xing-Quan Zhu



Abstract

Toxoplasma gondii secretes a number of virulence-related effector proteins, such as the rhoptry protein 18 (ROP18). To further broaden our understanding of the molecular functions of ROP18, we examined the transcriptional response of human embryonic kidney cells (HEK293T) to ROP18 of type I T. gondii RH strain. Using RNA-sequencing, we compared the transcriptome of ROP18-expressing HEK293T cells to control HEK293T cells. Our analysis revealed that ROP18 altered the expression of 750 genes (467 upregulated genes and 283 downregulated genes) in HEK293T cells. Gene ontology (GO) and pathway enrichment analyses showed that differentially expressed genes (DEGs) were significantly enriched in extracellular matrix– and immune–related GO terms and pathways. KEGG pathway enrichment analysis revealed that DEGs were involved in several disease-related pathways, such as nervous system diseases and eye disease. ROP18 significantly increased the alternative splicing pattern “retained intron” and altered the expression of 144 transcription factors (TFs). These results provide new insight into how ROP18 may influence biological processes in the host cells via altering the expression of genes, TFs, and pathways. More in vitro and in vivo studies are required to substantiate these findings.

Citation

Li, J., He, J., Elsheikha, H. M., Ma, J., Xu, X., & Zhu, X. (2020). ROP18-Mediated Transcriptional Reprogramming of HEK293T Cell Reveals New Roles of ROP18 in the Interplay Between Toxoplasma gondii and the Host Cell. Frontiers in Cellular and Infection Microbiology, 10, https://doi.org/10.3389/fcimb.2020.586946

Journal Article Type Article
Acceptance Date Oct 30, 2020
Online Publication Date Nov 30, 2020
Publication Date Nov 30, 2020
Deposit Date Dec 1, 2020
Publicly Available Date Dec 1, 2020
Journal Frontiers in Cellular and Infection Microbiology
Electronic ISSN 2235-2988
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 10
Article Number 586946
DOI https://doi.org/10.3389/fcimb.2020.586946
Public URL https://nottingham-repository.worktribe.com/output/5093852
Publisher URL https://www.frontiersin.org/articles/10.3389/fcimb.2020.586946/full

Files





You might also like



Downloadable Citations