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Prevalence of feline lungworm Aelurostrongylus abstrusus in England (2019)
Journal Article
Elsheikha, H. M., Wright, I., Wang, B., & Schaper, R. (2019). Prevalence of feline lungworm Aelurostrongylus abstrusus in England. Veterinary Parasitology, doi:10.1016/j.vprsr.2019.100271

Infection of cats with lungworm Aelurostrongylus abstrusus has recentlbeen documented in the UK. Here, we aimed to study the prevalence of A. abstrusus in fecal samples from cats across England. A total of 950 fecal samples were collected from cats t... Read More

Haemoproteus minutus is highly virulent for Australasian and South American parrots (2019)
Journal Article
Ortiz-Catedral, L., Brunton, D., Stidworthy, M. F., Elsheikha, H. M., Pennycott, T., Schulze, C., …Olias, P. (2019). Haemoproteus minutus is highly virulent for Australasian and South American parrots. Parasites and Vectors, 12(1), doi:10.1186/s13071-018-3255-0

Background: Haemoproteus and Plasmodium species are widespread avian blood parasites. Several Plasmodium species are known for their high virulence and have caused significant declines in naïve bird populations. The impact of closely related Haemopro... Read More

Transcriptomic insights into the early host-pathogen interaction of cat intestine with Toxoplasma gondii (2018)
Journal Article
Wang, M., Zhang, F., Elsheikha, H. M., Zhang, N., He, J., Luo, J., & Zhu, X. (2018). Transcriptomic insights into the early host-pathogen interaction of cat intestine with Toxoplasma gondii. Parasites and Vectors, 11(1), doi:10.1186/s13071-018-3179-8

Background: Although sexual reproduction of the parasite Toxoplasma gondii exclusively occurs in the cat intestine, knowledge about the alteration of gene expression in the intestine of cats infected with T. gondii is still limited. Here, we investig... Read More

Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures (2018)
Journal Article
Cong, W., Dottorini, T., Khan, F., Emes, R. D., Zhang, F., Zhou, C., …Zhu, X. (2018). Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures. Frontiers in Immunology, 9, doi:10.3389/fimmu.2018.02403. ISSN 1664-3224

RNA-sequencing was used to detect transcriptional changes in six tissues of cats, seven days after T. gondii infection. A total of 737 genes were differentially expressed (DEGs), of which 410 were up-regulated and 327 were down-regulated. The liver e... Read More

Prevalence of antibodies against Toxoplasma gondii in pets and their owners in Shandong province, Eastern China (2018)
Journal Article
Cong, W., Elsheikha, H. M., Zhou, N., Peng, P., Qin, S., Meng, Q., & Qian, A. (2018). Prevalence of antibodies against Toxoplasma gondii in pets and their owners in Shandong province, Eastern China. BMC Infectious Diseases, 18(1), doi:10.1186/s12879-018-3307-2

Background: Pet ownership in China has been steadily increasing over recent years. However, the risk of pet-associated zoonotic infection with the protozoan parasite Toxoplasma gondii remains poorly defined. Methods: In a cross-sectional survey, we... Read More

Functional characterization of dense granule proteins in Toxoplasma gondii RH strain using CRISPR-Cas9 system (2018)
Journal Article
Bai, M., Wang, J., Elsheikha, H. M., Liang, Q., Chen, K., Nie, L., & Zhu, X. (2018). Functional characterization of dense granule proteins in Toxoplasma gondii RH strain using CRISPR-Cas9 system. Frontiers in Cellular and Infection Microbiology, 8, (1-9). doi:10.3389/fcimb.2018.00300. ISSN 2235-2988

Infection with the apicomplexan protozoan parasite Toxoplasma gondii is an ongoing public health problem. The parasite's ability to invade and replicate within the host cell is dependent on many effectors, such as dense granule proteins (GRAs) releas... Read More

Optical sectioning in multi-foci Raman hyperspectral imaging (2018)
Journal Article
Liao, Z., Sinjab, F., Elsheikha, H. M., & Notingher, I. (in press). Optical sectioning in multi-foci Raman hyperspectral imaging. Journal of Raman Spectroscopy, doi:10.1002/jrs.5450. ISSN 0377-0486

In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was empl... Read More

Toxocariasis: a silent threat with a progressive public health impact (2018)
Journal Article
Chen, J., Liu, Q., Liu, G., Zheng, W., Hong, S., Sugiyama, H., …Elsheikha, H. M. (in press). Toxocariasis: a silent threat with a progressive public health impact. Infectious Diseases of Poverty, 7(1), doi:10.1186/s40249-018-0437-0. ISSN 2049-9957

Background: Toxocariasis is a neglected parasitic zoonosis that afflicts millions of the pediatric and adolescent populations worldwide, especially in impoverished communities. This disease is caused by infection with the larvae of Toxocara canis and... Read More

Hepatic metabolomics investigation in acute and chronic murine toxoplasmosis (2018)
Journal Article
Chen, X., Elsheikha, H. M., Hu, R., Hu, G., Guo, S., Zhou, C., & Zhu, X. (2018). Hepatic metabolomics investigation in acute and chronic murine toxoplasmosis. Frontiers in Cellular and Infection Microbiology, 8, doi:10.3389/fcimb.2018.00189

Toxoplasma gondii poses a great threat to human health, with no approved vaccine available for the treatment of T. gondii infection. T. gondii infections are not limited to the brain, and may also affect other organs especially the liver. Identificat... Read More

Live-attenuated Pru: Δcdpk2 strain of Toxoplasma gondii protects against acute, chronic and congenital toxoplasmosis (2018)
Journal Article
Wang, J., Li, T., Elsheikha, H. M., Chen, K., Cong, W., Yang, W., …Zhu, X. (in press). Live-attenuated Pru: Δcdpk2 strain of Toxoplasma gondii protects against acute, chronic and congenital toxoplasmosis. Journal of Infectious Diseases, doi:10.1093/infdis/jiy211. ISSN 0022-1899

Background: The threat of Toxoplasma gondii infection in immunocompromised individuals and pregnant women necessitates the development of a safe and effective vaccine. Here, we examined the immune protection conferred by live-attenuated strain of T.... Read More

Identification of two novel host proteins interacting with Toxoplasma gondii 14-3-3 protein by yeast two-hybrid system (2018)
Journal Article
Li, F., Liu, Q., Elsheikha, H. M., Yang, W., Hou, J., & Zhu, X. (in press). Identification of two novel host proteins interacting with Toxoplasma gondii 14-3-3 protein by yeast two-hybrid system. Parasitology Research, doi:10.1007/s00436-018-5812-z. ISSN 0932-0113

Toxoplasma gondii deploys many effector proteins in order to hijack and manipulate host cell signaling pathways, allowing parasite colonization, subversion of immune responses, and disease progression. T. gondii effector protein 14-3-3 (Tg14-3-3) pro... Read More