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Toxoplasma gondii tkl1 Deletion Mutant Is a Promising Vaccine against Acute, Chronic, and Congenital Toxoplasmosis in Mice (2020)
Journal Article
Wang, J., Liang, Q., Li, T., He, J., Bai, M., Cao, X., …Zhu, X. (2020). Toxoplasma gondii tkl1 Deletion Mutant Is a Promising Vaccine against Acute, Chronic, and Congenital Toxoplasmosis in Mice. Journal of Immunology, 204(4), https://doi.org/10.4049/jimmunol.1900410

In this study, we generated a tkl1 deletion mutant in the Toxoplasma gondii type 1 RH (RHΔtkl1) strain and tested the protective efficacies of vaccination using RHΔtkl1 tachyzoites against acute, chronic, and congenital T. gondii infections in Kunmin... Read More about Toxoplasma gondii tkl1 Deletion Mutant Is a Promising Vaccine against Acute, Chronic, and Congenital Toxoplasmosis in Mice.

Antioxidant enzymes as biomarkers of Cu and Pb exposure in the ground spiders Lycosa terrestris and Pardosa birmanica (2019)
Journal Article
Elsheikha, H. M., Aziz, N., & Butt, A. (2020). Antioxidant enzymes as biomarkers of Cu and Pb exposure in the ground spiders Lycosa terrestris and Pardosa birmanica. Ecotoxicology and Environmental Safety, 190, https://doi.org/10.1016/j.ecoenv.2019.110054

© 2019 Elsevier Inc. Heavy metal exposure induces oxidative stress in terrestrial organisms, which they counteract via activation of antioxidant biomarkers. The present study investigated the effects of copper (Cu) and lead (Pb) on the total antioxid... Read More about Antioxidant enzymes as biomarkers of Cu and Pb exposure in the ground spiders Lycosa terrestris and Pardosa birmanica.

Characterization of the Role of Amylo-Alpha-1,6-Glucosidase Protein in the Infectivity of Toxoplasma gondii (2019)
Journal Article
Cao, X., Wang, J., Elsheikha, H. M., Li, T., Sun, L., Liang, Q., …Lin, R. (2019). Characterization of the Role of Amylo-Alpha-1,6-Glucosidase Protein in the Infectivity of Toxoplasma gondii. Frontiers in Cellular and Infection Microbiology, 9, https://doi.org/10.3389/fcimb.2019.00418

In this study, we characterized the role of amylo-alpha-1,6-glucosidase (Aa16GL) in the biology and infectivity of Toxoplasma gondii, using Aa16GL-deficient parasites of type I RH and type II Prugniaud (Pru) strains. The subcellular localization of A... Read More about Characterization of the Role of Amylo-Alpha-1,6-Glucosidase Protein in the Infectivity of Toxoplasma gondii.

Prevalence, risk factors and genotype distribution of Toxoplasma gondii DNA in soil in China (2019)
Journal Article
Elsheikha, H. M., Cong, W., Zhu, X., Zhang, N., Fallaize, C. J., Hu, R., …Zou, Y. (2020). Prevalence, risk factors and genotype distribution of Toxoplasma gondii DNA in soil in China. Ecotoxicology and Environmental Safety, 189, https://doi.org/10.1016/j.ecoenv.2019.109999

Copyright © 2019 Elsevier Inc. All rights reserved. In the present study, we performed a cross-sectional survey to determine the occurrence and genotype distribution of T. gondii DNA in soil samples collected from different sources from six geographi... Read More about Prevalence, risk factors and genotype distribution of Toxoplasma gondii DNA in soil in China.

Label-Free Quantitative Acetylome Analysis Reveals Toxoplasma gondii Genotype-Specific Acetylomic Signatures (2019)
Journal Article
Wang, Z., Hu, R., Zhou, C., He, J., M. Elsheikha, H., & Zhu, X. (2019). Label-Free Quantitative Acetylome Analysis Reveals Toxoplasma gondii Genotype-Specific Acetylomic Signatures. Microorganisms, 7(11), 1-19. https://doi.org/10.3390/microorganisms7110510

Distinct genotypic and pathogenic differences exist between Toxoplasma gondii genotypes. For example, genotype I is highly virulent, whereas genotype II and genotype III are less virulent. Moreover, Chinese 1 genotype (ToxoDB#9) is also virulent. Her... Read More about Label-Free Quantitative Acetylome Analysis Reveals Toxoplasma gondii Genotype-Specific Acetylomic Signatures.

Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts (2019)
Journal Article
Zhang, X., Cwiklinski, K., Hu, R., Zheng, W., Sheng, Z., Zhang, F., …Zhu, X. (2019). Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts. BMC Genomics, 20, 1-18. https://doi.org/10.1186/s12864-019-6103-5

Background The tropical liver fluke, Fasciola gigantica causes fasciolosis, an important disease of humans and livestock. We characterized dynamic transcriptional changes associated with the development of the parasite in its two hosts, the snail in... Read More about Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts.

Serum metabolomic alterations in Beagle dogs experimentally infected with Toxocara canis (2019)
Journal Article
Zou, Y., Zheng, W., Elsheikha, H. M., Liu, G., Hu, M., Wang, S., & Zhu, X. (2019). Serum metabolomic alterations in Beagle dogs experimentally infected with Toxocara canis. Parasites and Vectors, 12(1), 1-10. https://doi.org/10.1186/s13071-019-3703-5

Background Toxocara canis, a globally distributed roundworm, can cause debilitating disease in dogs and humans; however, little is known about the metabolomic response of the hosts to T. canis infection. There is an increasing need to understand the... Read More about Serum metabolomic alterations in Beagle dogs experimentally infected with Toxocara canis.

Metabolomic signature of mouse cerebral cortex following Toxoplasma gondii infection (2019)
Journal Article
Ma, J., He, J., Hou, J., Zhou, C., Zhang, F., Elsheikha, H. M., & Zhu, X. (2019). Metabolomic signature of mouse cerebral cortex following Toxoplasma gondii infection. Parasites and Vectors, 12, 1-11. doi:10.1186/s13071-019-3623-4

Background: The protozoan parasite Toxoplasma gondii infects and alters the neurotransmission in cerebral cortex and other brain regions, leading to neurobehavioral and neuropathologic changes in humans and animals. However, the molecules that contri... Read More about Metabolomic signature of mouse cerebral cortex following Toxoplasma gondii infection.

The multitasking Fasciola gigantica Cathepsin B interferes with various functions of goat peripheral blood mononuclear cells in vitro (2019)
Journal Article
Chen, D., Tian, A., Hou, J., Li, J., Tian, X., Yuan, X., …Zhu, X. (2019). The multitasking Fasciola gigantica Cathepsin B interferes with various functions of goat peripheral blood mononuclear cells in vitro. Frontiers in Immunology, 10, doi:10.3389/fimmu.2019.01707

Cathepsin B, a lysosomal cysteine protease, is thought to be involved in the pathogenesis of Fasciola gigantica infection, but its exact role remains unclear. In the present study, a recombinant F. gigantica cathepsin B (rFgCatB) protein was expresse... Read More about The multitasking Fasciola gigantica Cathepsin B interferes with various functions of goat peripheral blood mononuclear cells in vitro.

Global serum proteomic changes in water buffaloes infected with Fasciola gigantica (2019)
Journal Article
Zhang, F., Hu, R., Elsheikha, H. M., Sheng, Z., Zhang, W., Zheng, W., …He, J. (2019). Global serum proteomic changes in water buffaloes infected with Fasciola gigantica. Parasites and Vectors, 12(1), doi:10.1186/s13071-019-3533-5

Background: The liver fluke Fasciola gigantica modulates several signaling pathways in infected buffaloes to facilitate its survival and establishment of persistent infection. In response to the parasite invasion, buffaloes activate innate and adapti... Read More about Global serum proteomic changes in water buffaloes infected with Fasciola gigantica.

New insights into the mechanical properties of Acanthamoeba castellanii cysts as revealed by phonon microscopy (2019)
Journal Article
Pérez-Cota, F., Smith, R. J., Elsheikha, H. M., & Clark, M. (2019). New insights into the mechanical properties of Acanthamoeba castellanii cysts as revealed by phonon microscopy. Biomedical Optics Express, 10(5), 2399-2408. doi:10.1364/BOE.10.002399

The single cell eukaryotic protozoan Acanthamoeba castellanii exhibits a remarkable ability to switch from a vegetative trophozoite stage to a cystic form, in response to stressors. This phenotypic switch involves changes in gene expression and synth... Read More about New insights into the mechanical properties of Acanthamoeba castellanii cysts as revealed by phonon microscopy.

Label-free characterization of biochemical changes within human cells under parasite attack using synchrotron based micro-FTIR (2019)
Journal Article
Elsheikha, H. M., Elsaied, N. A., Chan, K. L. A., Brignell, C., Harun, M. S. R., Wehbe, K., & Cinquee, G. (2019). Label-free characterization of biochemical changes within human cells under parasite attack using synchrotron based micro-FTIR. Analytical Methods, 11(19), 2518-2530. doi:10.1039/c8ay02777c

The protozoan Toxoplasma gondii is responsible for severe, potentially life-threatening, infection in immunocompromised individuals and when acquired during pregnancy. In the meantime, there is no available vaccine and the anti-T. gondii drug arsenal... Read More about Label-free characterization of biochemical changes within human cells under parasite attack using synchrotron based micro-FTIR.

Prevalence of feline lungworm Aelurostrongylus abstrusus in England (2019)
Journal Article
Elsheikha, H. M., Wright, I., Wang, B., & Schaper, R. (2019). Prevalence of feline lungworm Aelurostrongylus abstrusus in England. Veterinary parasitology (Amsterdam), 16, 100271. https://doi.org/10.1016/j.vprsr.2019.100271

Infection of cats with lungworm Aelurostrongylus abstrusus has recentlbeen documented in the UK. Here, we aimed to study the prevalence of A. abstrusus in fecal samples from cats across England. A total of 950 fecal samples were collected from cats t... Read More about Prevalence of feline lungworm Aelurostrongylus abstrusus in England.

Haemoproteus minutus is highly virulent for Australasian and South American parrots (2019)
Journal Article
Ortiz-Catedral, L., Brunton, D., Stidworthy, M. F., Elsheikha, H. M., Pennycott, T., Schulze, C., …Olias, P. (2019). Haemoproteus minutus is highly virulent for Australasian and South American parrots. Parasites and Vectors, 12(1), doi:10.1186/s13071-018-3255-0

Background: Haemoproteus and Plasmodium species are widespread avian blood parasites. Several Plasmodium species are known for their high virulence and have caused significant declines in naïve bird populations. The impact of closely related Haemopro... Read More about Haemoproteus minutus is highly virulent for Australasian and South American parrots.

Assessment of bioaccumulation of cu and Pb in experimentally exposed spiders, Lycosa terrestris and Pardosa birmanica, using different exposure routes (2019)
Journal Article
Aziz, N., Butt, A., & Elsheikha, H. M. (2019). Assessment of bioaccumulation of cu and Pb in experimentally exposed spiders, Lycosa terrestris and Pardosa birmanica, using different exposure routes. Environmental Science and Pollution Research, https://doi.org/10.1007/s11356-019-07055-0

© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Major concerns exist regarding the environmental and human health risks caused by exposure to heavy metals. Spiders are often used as a model in ecotoxicological studies to assess soil po... Read More about Assessment of bioaccumulation of cu and Pb in experimentally exposed spiders, Lycosa terrestris and Pardosa birmanica, using different exposure routes.

Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system (2019)
Journal Article
Sinjab, F., Elsheikha, H. M., Dooley, M., & Notingher, I. (2019). Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system. Journal of Biophotonics, https://doi.org/10.1002/jbio.201960065

© 2019 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Understanding and quantifying the temporal acquisition of host cell molecules by intracellular pathogens is fundamentally important in biology. In t... Read More about Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system.

Transcriptomic insights into the early host-pathogen interaction of cat intestine with Toxoplasma gondii (2018)
Journal Article
Wang, M., Zhang, F., Elsheikha, H. M., Zhang, N., He, J., Luo, J., & Zhu, X. (2018). Transcriptomic insights into the early host-pathogen interaction of cat intestine with Toxoplasma gondii. Parasites and Vectors, 11(1), doi:10.1186/s13071-018-3179-8

Background: Although sexual reproduction of the parasite Toxoplasma gondii exclusively occurs in the cat intestine, knowledge about the alteration of gene expression in the intestine of cats infected with T. gondii is still limited. Here, we investig... Read More about Transcriptomic insights into the early host-pathogen interaction of cat intestine with Toxoplasma gondii.

The pervasive effects of recombinant Fasciola gigantica Ras-related protein Rab10 on the functions of goat peripheral blood mononuclear cells (2018)
Journal Article
Tian, A., Lu, M., Zhang, F., Calderón-Mantilla, G., Petsalaki, E., Tian, X., …Zhu, X. (2018). The pervasive effects of recombinant Fasciola gigantica Ras-related protein Rab10 on the functions of goat peripheral blood mononuclear cells. Parasites and Vectors, 11, doi:10.1186/s13071-018-3148-2

Background: Fasciola gigantica-induced immunomodulation is a major hurdle faced by the host for controlling infection. Here, we elucidated the role of F. gigantica Ras-related protein Rab10 (FgRab10) in the modulation of key functions of peripheral b... Read More about The pervasive effects of recombinant Fasciola gigantica Ras-related protein Rab10 on the functions of goat peripheral blood mononuclear cells.