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Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica

Shi, Wei; He, Jun-Jun; Mei, Xue-Fang; Lu, Ke-Jing; Zeng, Zi-Xuan; Zhang, Yao-Yao; Sheng, Zhao-An; Elsheikha, Hany M.; Huang, Wei-Yi; Zhu, Xing-Quan

Authors

Wei Shi

Jun-Jun He

Xue-Fang Mei

Ke-Jing Lu

Zi-Xuan Zeng

Yao-Yao Zhang

Zhao-An Sheng

Wei-Yi Huang

Xing-Quan Zhu



Contributors

Sutas Suttiprapa
Editor

Abstract

The excretory-secretory products released by the liver fluke Fasciola gigantica (FgESPs) play important roles in regulating the host immune response during the infection. Identification of hepatic miRNAs altered by FgESPs may improve our understanding of the pathogenesis of F. gigantica infection. In this study, we investigated the alterations in the hepatic microRNAs (miRNAs) in mice treated with FgESPs using high-throughput small RNA (sRNA) sequencing and bioinformatics analysis. The expression of seven miRNAs was confirmed by quantitative stem-loop reverse transcription quantitative PCR (qRT-PCR). A total of 1,313 miRNAs were identified in the liver of mice, and the differentially expressed (DE) miRNAs varied across the time lapsed post exposure to FgESPs. We identified 67, 154 and 53 dysregulated miRNAs at 1, 4 and 12 weeks post-exposure, respectively. 5 miRNAs (miR-126a-3p, miR-150-5p, miR-155-5p, miR-181a-5p and miR-362-3p) were commonly dysregulated at the three time points. We also found that most of the DE miRNAs were induced by FgESPs in the mouse liver after 4 weeks of exposure. These were subjected to Gene Ontology (GO) enrichment analysis, which showed that the predicted targets of the hepatic DE miRNAs of mice 4 weeks of FgESPs injection were enriched in GO terms, including cell membrane, ion binding, cellular communication, organelle and DNA damage. KEGG analysis indicated that the predicted targets of the most downregulated miRNAs were involved in 15 neural activity-related pathways, 6 digestion-related pathways, 20 immune response-related pathways and 17 cancer-related pathways. These data provide new insights into how FgESPs can dysregulate hepatic miRNAs, which play important roles in modulating several aspects of F. gigantica pathogenesis.

Citation

Shi, W., He, J., Mei, X., Lu, K., Zeng, Z., Zhang, Y., …Zhu, X. (2020). Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica. PLoS Neglected Tropical Diseases, 14(12), https://doi.org/10.1371/journal.pntd.0008951

Journal Article Type Article
Acceptance Date Nov 3, 2020
Online Publication Date Dec 17, 2020
Publication Date Dec 1, 2020
Deposit Date Dec 19, 2020
Publicly Available Date Jan 11, 2021
Journal PLoS neglected tropical diseases
Electronic ISSN 1935-2735
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 14
Issue 12
Article Number e0008951
DOI https://doi.org/10.1371/journal.pntd.0008951
Keywords Public Health, Environmental and Occupational Health; Infectious Diseases
Public URL https://nottingham-repository.worktribe.com/output/5157288
Publisher URL https://doi.org/10.1371/journal.pntd.0008951

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