Lenu B. Fakae
In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii
Fakae, Lenu B.; Stevenson, Carl W.; Zhu, Xing Quan; Elsheikha, Hany M.
Authors
Dr CARL STEVENSON carl.stevenson@nottingham.ac.uk
Associate Professor
Xing Quan Zhu
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Abstract
© 2020 The Author(s) The effect of Camellia sinensis (green tea) on the growth of Acanthamoeba castellanii trophozoites was examined using a microplate based-Sulforhodamine B (SRB) assay. C. sinensis hot and cold brews at 75% and 100% concentrations significantly inhibited the growth of trophozoites. We also examined the structural alterations in C. sinensis-treated trophozoites using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). This analysis showed that C. sinensis compromised the cell membrane integrity and caused progressive destruction of trophozoites. C. sinensis also significantly inhibited the parasite's ability to form cysts in a dose-dependent manner and reduced the rate of excystation from cysts to trophozoites. C. sinensis exhibited low cytotoxic effects on primary corneal stromal cells. However, cytotoxicity was more pronounced in SV40-immortalized corneal epithelial cells. Chromatographic analysis showed that both hot and cold C. sinensis brews contained the same number and type of chemical compounds. This work demonstrated that C. sinensis has anti-acanthamoebic activity against trophozoite and cystic forms of A. castellanii. Further studies are warranted to identify the exact substances in C. sinensis that have the most potent anti-acanthamoebic effect.
Citation
Fakae, L. B., Stevenson, C. W., Zhu, X. Q., & Elsheikha, H. M. (2020). In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii. International Journal for Parasitology: Drugs and Drug Resistance, 13, 59-72. https://doi.org/10.1016/j.ijpddr.2020.05.001
Journal Article Type | Article |
---|---|
Acceptance Date | May 12, 2020 |
Online Publication Date | Jun 2, 2020 |
Publication Date | Aug 1, 2020 |
Deposit Date | Jun 6, 2020 |
Publicly Available Date | Mar 29, 2024 |
Journal | International Journal for Parasitology: Drugs and Drug Resistance |
Print ISSN | 2211-3207 |
Electronic ISSN | 2211-3207 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 13 |
Pages | 59-72 |
DOI | https://doi.org/10.1016/j.ijpddr.2020.05.001 |
Keywords | Pharmacology (medical); Pharmacology; Parasitology; Infectious Diseases |
Public URL | https://nottingham-repository.worktribe.com/output/4597220 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S2211320720300117 |
Additional Information | This article is maintained by: Elsevier; Article Title: In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii; Journal Title: International Journal for Parasitology: Drugs and Drug Resistance; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.ijpddr.2020.05.001; Content Type: article; Copyright: © 2020 The Author(s). Published by Elsevier Ltd on behalf of Australian Society for Parasitology. |
Files
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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