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In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii

Fakae, Lenu B.; Stevenson, Carl W.; Zhu, Xing Quan; Elsheikha, Hany M.

Authors

Lenu B. Fakae

Xing Quan Zhu



Abstract

© 2020 The Author(s) The effect of Camellia sinensis (green tea) on the growth of Acanthamoeba castellanii trophozoites was examined using a microplate based-Sulforhodamine B (SRB) assay. C. sinensis hot and cold brews at 75% and 100% concentrations significantly inhibited the growth of trophozoites. We also examined the structural alterations in C. sinensis-treated trophozoites using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). This analysis showed that C. sinensis compromised the cell membrane integrity and caused progressive destruction of trophozoites. C. sinensis also significantly inhibited the parasite's ability to form cysts in a dose-dependent manner and reduced the rate of excystation from cysts to trophozoites. C. sinensis exhibited low cytotoxic effects on primary corneal stromal cells. However, cytotoxicity was more pronounced in SV40-immortalized corneal epithelial cells. Chromatographic analysis showed that both hot and cold C. sinensis brews contained the same number and type of chemical compounds. This work demonstrated that C. sinensis has anti-acanthamoebic activity against trophozoite and cystic forms of A. castellanii. Further studies are warranted to identify the exact substances in C. sinensis that have the most potent anti-acanthamoebic effect.

Journal Article Type Article
Publication Date Aug 1, 2020
Journal International Journal for Parasitology: Drugs and Drug Resistance
Print ISSN 2211-3207
Electronic ISSN 2211-3207
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 13
Pages 59-72
APA6 Citation Fakae, L. B., Stevenson, C. W., Zhu, X. Q., & Elsheikha, H. M. (2020). In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii. International Journal for Parasitology: Drugs and Drug Resistance, 13, 59-72. https://doi.org/10.1016/j.ijpddr.2020.05.001
DOI https://doi.org/10.1016/j.ijpddr.2020.05.001
Keywords Pharmacology (medical); Pharmacology; Parasitology; Infectious Diseases
Publisher URL https://www.sciencedirect.com/science/article/pii/S2211320720300117
Additional Information This article is maintained by: Elsevier; Article Title: In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii; Journal Title: International Journal for Parasitology: Drugs and Drug Resistance; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.ijpddr.2020.05.001; Content Type: article; Copyright: © 2020 The Author(s). Published by Elsevier Ltd on behalf of Australian Society for Parasitology.

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