Rui-Si Hu
Transcriptomic Profiling of Mouse Brain During Acute and Chronic Infections by Toxoplasma gondii Oocysts
Hu, Rui-Si; He, Jun-Jun; Elsheikha, Hany M.; Zou, Yang; Ehsan, Muhammad; Ma, Qiao-Ni; Zhu, Xing-Quan; Cong, Wei
Authors
Jun-Jun He
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Yang Zou
Muhammad Ehsan
Qiao-Ni Ma
Xing-Quan Zhu
Wei Cong
Abstract
Infection by the protozoan Toxoplasma gondii can have a devastating impact on the structure and function of the brain of the infected individuals, particularly immunocompromised patients. A systems biology view of the brain transcriptome can identify key molecular targets and pathways that mediate the neuropathogenesis of cerebral toxoplasmosis. Here, we performed transcriptomic analysis of the brain of mice infected by T. gondii Pru strain oocysts at 11 and 33 days post-infection (dpi) compared to uninfected (control) mice using RNA sequencing (RNA-seq). T. gondii altered the expression of 936 and 2,081 transcripts at 11 and 33 dpi, respectively, and most of these were upregulated in the infected brains. Gene Ontology (GO) enrichment and pathway analysis showed that immune response, such as interferon-gamma (IFN-?) responsive genes were strongly affected at 11dpi. Likewise, differentially expressed transcripts (DETs) related to T cell activation, cytokine production and immune cell proliferation were significantly altered at 33 dpi. Host-parasite interactome analysis showed that some DETs were involved in immune signaling, metabolism, biosynthesis-related processes and interspecies interaction. These findings should increase knowledge of the mouse brain transcriptome and the changes in transcriptional regulation and downstream signaling pathways during acute and chronic T. gondii infections.
Citation
Hu, R., He, J., Elsheikha, H. M., Zou, Y., Ehsan, M., Ma, Q., …Cong, W. (2020). Transcriptomic Profiling of Mouse Brain During Acute and Chronic Infections by Toxoplasma gondii Oocysts. Frontiers in Microbiology, 11, Article 570903. https://doi.org/10.3389/fmicb.2020.570903
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 21, 2020 |
Online Publication Date | Oct 19, 2020 |
Publication Date | Oct 19, 2020 |
Deposit Date | Oct 19, 2020 |
Publicly Available Date | Oct 19, 2020 |
Journal | Frontiers in Microbiology |
Electronic ISSN | 1664-302X |
Publisher | Frontiers Media |
Peer Reviewed | Peer Reviewed |
Volume | 11 |
Article Number | 570903 |
DOI | https://doi.org/10.3389/fmicb.2020.570903 |
Keywords | Microbiology (medical); Microbiology |
Public URL | https://nottingham-repository.worktribe.com/output/4976783 |
Publisher URL | https://www.frontiersin.org/articles/10.3389/fmicb.2020.570903/full |
Files
Transcriptomic Profiling of Mouse Brain
(9.1 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
You might also like
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: digital-library-support@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search