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Acetylome analysis of the feline small intestine following Toxoplasma gondii infection

Meng, Yu-Meng; Zhai, Bin-Tao; Elsheikha, Hany M.; Xie, Shi-Chen; Wang, Ze-Xiang; Zhao, Quan; Zhu, Xing-Quan; He, Jun-Jun

Acetylome analysis of the feline small intestine following Toxoplasma gondii infection Thumbnail


Authors

Yu-Meng Meng

Bin-Tao Zhai

Shi-Chen Xie

Ze-Xiang Wang

Quan Zhao

Xing-Quan Zhu

Jun-Jun He



Abstract

Toxoplasma gondii is a protozoan parasite capable of infecting a large number of warm-blooded animals and causes serious health complications in immunocompromised patients. T. gondii infection of the feline small intestine is critical for the completion of the life cycle and transmission of T. gondii. Protein acetylation is an important posttranslational modification, which plays roles in the regulation of various cellular processes. Therefore, understanding of how T. gondii reprograms the protein acetylation status of feline definitive host can help to thwart the production and spread of T. gondii. Here, we used affinity enrichment and high-resolution liquid chromatography with tandem mass spectrometry to profile the alterations of the acetylome in cat small intestine 10 days after infection by T. gondii Prugniuad (Pru) strain. Our analysis showed that T. gondii induced significant changes in the acetylation of proteins in the cat intestine. We identified 2606 unique lysine acetylation sites in 1357 acetylated proteins. The levels of 334 acetylated peptides were downregulated, while the levels of 82 acetylated peptides were increased in the infected small intestine. The proteins with differentially acetylated peptides were particularly enriched in the bioenergetics-related processes, such as tricarboxylic acid cycle, oxidative phosphorylation, and oxidation-reduction. These results provide the first baseline of the global acetylome of feline small intestine following T. gondii infection and should facilitate further analysis of the role of acetylated protein in the pathogenesis of T. gondii infection in its definitive host.

Citation

Meng, Y., Zhai, B., Elsheikha, H. M., Xie, S., Wang, Z., Zhao, Q., …He, J. (2020). Acetylome analysis of the feline small intestine following Toxoplasma gondii infection. Parasitology Research, 119(11), 3649-3657. https://doi.org/10.1007/s00436-020-06880-4

Journal Article Type Article
Acceptance Date Sep 7, 2020
Online Publication Date Sep 20, 2020
Publication Date Nov 1, 2020
Deposit Date Oct 23, 2020
Publicly Available Date Mar 28, 2024
Journal Parasitology Research
Print ISSN 0932-0113
Electronic ISSN 1432-1955
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 119
Issue 11
Pages 3649-3657
DOI https://doi.org/10.1007/s00436-020-06880-4
Keywords Insect Science; General Veterinary; Parasitology; Infectious Diseases; General Medicine
Public URL https://nottingham-repository.worktribe.com/output/4985747
Publisher URL https://link.springer.com/article/10.1007/s00436-020-06880-4
Additional Information Meng, Y., Zhai, B., Elsheikha, H.M. et al. Acetylome analysis of the feline small intestine following Toxoplasma gondii infection. Parasitol Res 119, 3649–3657 (2020). https://doi.org/10.1007/s00436-020-06880-4

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