Structural, functional, and metabolic alterations in human cerebrovascular endothelial cells during toxoplasma gondii infection and amelioration by verapamil in vitro

Al-Sandaqchi, Alaa T.; Marsh, Victoria; Williams, Huw E. L.; Stevenson, Carl W.; Elsheikha, Hany M.

Abstract

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Toxoplasma gondii (T. gondii), the causative agent of toxoplasmosis, is a frequent cause of brain infection. Despite its known ability to invade the brain, there is still a dire need to better understand the mechanisms by which this parasite interacts with and crosses the blood–brain barrier (BBB). The present study revealed structural and functional changes associated with infection and replication of T. gondii within human brain microvascular endothelial cells (BMECs) in vitro. T. gondii proliferated within the BMECs and disrupted the integrity of the cerebrovascular barrier through diminishing the cellular viability, disruption of the intercellular junctions and increasing permeability of the BMEC monolayer, as well as altering lipid homeostasis. Proton nuclear magnetic resonance (1H NMR)-based metabolomics combined with multivariate data analysis revealed profiles that can be attributed to infection and variations in the amounts of certain metabolites (e.g., amino acids, fatty acids) in the extracts of infected compared to control cells. Notably, treatment with the Ca2+ channel blocker verapamil rescued BMEC barrier integrity and restricted intracellular replication of the tachyzoites regardless of the time of treatment application (i.e., prior to infection, early-and late-infection). This study provides new insights into the structural and functional changes that accompany T. gondii infection of the BMECs, and sheds light upon the ability of verapamil to inhibit the parasite proliferation and to ameliorate the adverse effects caused by T. gondii infection.

Citation

Al-Sandaqchi, A. T., Marsh, V., Williams, H. E. L., Stevenson, C. W., & Elsheikha, H. M. (2020). Structural, functional, and metabolic alterations in human cerebrovascular endothelial cells during toxoplasma gondii infection and amelioration by verapamil in vitro. Microorganisms, 8(9), Article 1386. https://doi.org/10.3390/microorganisms8091386

• Journal Article Type: Article
• Acceptance Date: Sep 8, 2020
• Online Publication Date: Sep 10, 2020
• Publication Date: Sep 1, 2020
• Deposit Date: Sep 11, 2020
• Publicly Available Date: Sep 11, 2020
• Journal: Microorganisms
• Electronic ISSN: 2076-2607
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 9
• Article Number: 1386
• DOI: https://doi.org/10.3390/microorganisms8091386
• Keywords: Toxoplasma gondii; blood–brain barrier; host response; host-pathogen interaction; metabolomics; verapamil
• Public URL: https://nottingham-repository.worktribe.com/output/4896898
• Publisher URL: https://www.mdpi.com/2076-2607/8/9/1386
• Additional Information: Al-sandaqchi, A. T., Marsh, V., Williams, H. E. L., Stevenson, C. W., & Elsheikha, H. M. (2020). Structural, Functional, and Metabolic Alterations in Human Cerebrovascular Endothelial Cells during Toxoplasma gondii Infection and Amelioration by Verapamil In Vitro. Microorganisms, 8(9), 1386. https://doi.org/10.3390/microorganisms8091386