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A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells (2023)
Journal Article
Cuevas-Ocaña, S., Yang, J. Y., Aushev, M., Schlossmacher, G., Bear, C. E., Hannan, N. R., …Gray, M. A. (2023). A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells. International Journal of Molecular Sciences, 24(12), Article 10266. https://doi.org/10.3390/ijms241210266

Introducing or correcting disease-causing mutations through genome editing in human pluripotent stem cells (hPSCs) followed by tissue-specific differentiation provide sustainable models of multiorgan diseases, such as cystic fibrosis (CF). However, l... Read More about A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells.

Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate (2023)
Journal Article
Bardelang, P., Murray, E. J., Blower, I., Zandomeneghi, S., Goode, A., Hussain, R., …Bonev, B. B. (2023). Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate. Frontiers in Chemistry, 11, Article 1113885. https://doi.org/10.3389/fchem.2023.1113885

Virulence gene expression in the human pathogen, S. aureus is regulated by the agr (accessory gene regulator) quorum sensing (QS) system which is conserved in diverse Gram-positive bacteria. The agr QS signal molecule is an autoinducing peptide (AIP)... Read More about Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate.

Anti-miR-135/SPOCK1 axis antagonizes the influence of metabolism on drug response in intestinal/colon tumour organoids (2022)
Journal Article
Babaei-Jadidi, R., Kashfi, H., Alelwani, W., Bakhtiari, A., Kattan, S. W., Mansouri, O. A., …Nateri, A. S. (2022). Anti-miR-135/SPOCK1 axis antagonizes the influence of metabolism on drug response in intestinal/colon tumour organoids. Oncogenesis, 11, Article 4. https://doi.org/10.1038/s41389-021-00376-1

Little is known about the role of microRNAs (miRNAs) in rewiring the metabolism within tumours and adjacent non-tumour bearing normal tissue and their potential in cancer therapy. This study aimed to investigate the relationship between deregulated m... Read More about Anti-miR-135/SPOCK1 axis antagonizes the influence of metabolism on drug response in intestinal/colon tumour organoids.

Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin (2021)
Journal Article
Tarantini, F. S., Brunati, M., Taravella, A., Carrano, L., Parenti, F., Hong, K. W., …Chan, W. C. (2021). Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin. PLoS ONE, 16(11), Article e0260413. https://doi.org/10.1371/journal.pone.0260413

As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the mo... Read More about Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin.

Shape changes and cooperativity in the folding of central domain of the 16S ribosomal RNA (2021)
Journal Article
Hori, N., Denesyuk, N. A., & Thirumalai, D. (2021). Shape changes and cooperativity in the folding of central domain of the 16S ribosomal RNA. Proceedings of the National Academy of Sciences, 118(10), Article e2020837118. https://doi.org/10.1073/pnas.2020837118

Both the small and large subunits of the ribosome, the molecular machine that synthesizes proteins, are complexes of ribosomal RNAs (rRNAs) and a number of proteins. In bacteria, the small subunit has a single 16S rRNA whose folding is the first step... Read More about Shape changes and cooperativity in the folding of central domain of the 16S ribosomal RNA.

Cordycepin, a metabolite of Cordyceps militaris, reduces immune-related gene expression in insects (2020)
Journal Article
Woolley, V. C., Teakle, G. R., Prince, G., de Moor, C. H., & Chandler, D. (2020). Cordycepin, a metabolite of Cordyceps militaris, reduces immune-related gene expression in insects. Journal of Invertebrate Pathology, 177, Article 107480. https://doi.org/10.1016/j.jip.2020.107480

Hypocrealean entomopathogenic fungi (EPF) (Sordariomycetes, Ascomycota) are natural regulators of insect populations in terrestrial environments. Their obligately-killing life-cycle means that there is likely to be strong selection pressure for trait... Read More about Cordycepin, a metabolite of Cordyceps militaris, reduces immune-related gene expression in insects.

Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus (2020)
Journal Article
Blower, I., Tong, C., Sun, X., Murray, E., Luckett, J., Chan, W., …Hill, P. (2020). Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus. Sensors, 20(15), 1-12. https://doi.org/10.3390/s20154305

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Gaussia luciferase (GLuc) is a secreted protein with significant potential for use as a reporter of gene expression in bacterial pathogenicity studies. To date there are relatively few example... Read More about Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus.

Metabolism based isolation of invasive glioblastoma cells with specific gene signatures and tumorigenic potential (2020)
Journal Article
Smith, S. J., Rowlinson, J., Estevez-Cebrero, M., Onion, D., Ritchie, A., Clarke, P., …Rahman, R. (2020). Metabolism based isolation of invasive glioblastoma cells with specific gene signatures and tumorigenic potential. Neuro-Oncology Advances, 2(1), Article vdaa087. https://doi.org/10.1093/noajnl/vdaa087

Background Glioblastoma (GBM) is a highly aggressive brain tumor with rapid subclonal diversification, harboring molecular abnormalities that vary temporo-spatially, a contributor to therapy resistance. Fluorescence guided neurosurgical resection ut... Read More about Metabolism based isolation of invasive glioblastoma cells with specific gene signatures and tumorigenic potential.

The Differential Expression of ERAP1/ERAP2 and Immune Cell Activation in Pre-eclampsia (2020)
Journal Article
Stratikos, E., Warthan, M., Strauss, J. F., Kurlak, L. O., Seamon, K., Lee, E. D., & Mistry, H. D. (2020). The Differential Expression of ERAP1/ERAP2 and Immune Cell Activation in Pre-eclampsia. Frontiers in Immunology, 11, Article 396. https://doi.org/10.3389/fimmu.2020.00396

Pre-eclampsia (PE) is a disorder of pregnancy, often leading to serious and fatal complications. Endoplasmic reticulum aminopeptidase 1 and 2 (ERAP1/ERAP2) are present in the placenta. They are involved in processes regulating blood pressure, angioge... Read More about The Differential Expression of ERAP1/ERAP2 and Immune Cell Activation in Pre-eclampsia.

Using Light for Therapy of Glioblastoma Multiforme (GBM) (2020)
Journal Article
Kasparov, Vasilev, Teschemacher, Sofi, Smith, & Rahman, R. (2020). Using Light for Therapy of Glioblastoma Multiforme (GBM). Brain Sciences, 10(2), Article 75. https://doi.org/10.3390/brainsci10020075

Glioblastoma multiforme (GBM) is the most malignant form of primary brain tumour with extremely poor prognosis. The current standard of care for newly diagnosed GBM includes maximal surgical resection followed by radiotherapy and adjuvant chemotherap... Read More about Using Light for Therapy of Glioblastoma Multiforme (GBM).

A role for ABCB1 in prognosis, invasion and drug resistance in ependymoma (2019)
Journal Article
Sabnis, D. H., Storer, L. C. D., Liu, J., Jackson, H. K., Kilday, J. P., Grundy, R. G., …Coyle, B. (2019). A role for ABCB1 in prognosis, invasion and drug resistance in ependymoma. Scientific Reports, 9(1), 1-9. https://doi.org/10.1038/s41598-019-46700-z

© 2019, The Author(s). Three of the hallmarks of poor prognosis in paediatric ependymoma are drug resistance, local invasion and recurrence. We hypothesised that these hallmarks were due to the presence of a sub-population of cancer stem cells expres... Read More about A role for ABCB1 in prognosis, invasion and drug resistance in ependymoma.

An in-vivo pilot study into the effects of FDG-mNP in cancer in mice (2018)
Journal Article
Aras, O., Pearce, G., Watkins, A. J., Nurili, F., Medine, E. I., Guldu, O. K., …Akin, O. (2018). An in-vivo pilot study into the effects of FDG-mNP in cancer in mice. PLoS ONE, 13(8), Article e0202482. https://doi.org/10.1371/journal.pone.0202482

PURPOSE: Previously, fluorodeoxy glucose conjugated magnetite nanoparticles (FDG-mNPs) injected into cancer cells in conjunction with the application of magnetic hyperthermia have shown promise in new FDG-mNPs applications. The aim of this study was... Read More about An in-vivo pilot study into the effects of FDG-mNP in cancer in mice.

Cancer reversion with oocyte extracts is mediated by cell cycle arrest and induction of tumour dormancy (2018)
Journal Article
Saad, N., Alberio, R., Johnson, A. D., Emes, R. D., Giles, T. C., Clarke, P., …Allegrucci, C. (2018). Cancer reversion with oocyte extracts is mediated by cell cycle arrest and induction of tumour dormancy. Oncotarget, 9(22), 16008-16027. https://doi.org/10.18632/oncotarget.24664

Inducing stable control of tumour growth by tumour reversion is an alternative approach to cancer treatment when eradication of the disease cannot be achieved. The process requires re-establishment of normal control mechanisms that are lost in canc... Read More about Cancer reversion with oocyte extracts is mediated by cell cycle arrest and induction of tumour dormancy.

Androgen dependent mechanisms of pro-angiogenic networks in placental and tumor development (2017)
Journal Article
Metzler, V. M., de Brot, S., Robinson, R. S., Jeyapalan, J. N., Rakha, E., Walton, T., …Mongan, N. P. (2017). Androgen dependent mechanisms of pro-angiogenic networks in placental and tumor development. Placenta, 56, 79-85. https://doi.org/10.1016/j.placenta.2017.02.018

The placenta and tumors share important characteristics, including a requirement to establish effective angiogenesis. In the case of the placenta, optimal angiogenesis is required to sustain the blood flow required to maintain a successful pregnancy,... Read More about Androgen dependent mechanisms of pro-angiogenic networks in placental and tumor development.

HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells (2017)
Journal Article
Shah, M., Cardenas, R., Wang, B., Persson, J., Mongan, N. P., Grabowska, A. M., & Allegrucci, C. (2017). HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells. Molecular Cancer, 16(38), https://doi.org/10.1186/s12943-017-0605-z

Background: Homeobox genes are master regulators of cell fate during embryonic development and their expression is altered in cancer. By regulating the balance between cell proliferation and differentiation, they maintain homeostasis of normal tissue... Read More about HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells.

Cyclin A1 and P450 aromatase promote metastatic homing and growth of stem-like prostate cancer cells in the bone marrow (2016)
Journal Article
Miftakhova, R., Hedblom, A., Semenas, J., Robinson, B. D., Simoulis, A., Malm, J., …Persson, J. L. (2016). Cyclin A1 and P450 aromatase promote metastatic homing and growth of stem-like prostate cancer cells in the bone marrow. Cancer Research, 76(8), 2453-2464. https://doi.org/10.1158/0008-5472.CAN-15-2340

Bone metastasis is a leading cause of morbidity and mortality in prostate cancer (PCa). While cancer stem-like cells have been implicated as a cell of origin for PCa metastases, the pathways which enable metastatic development at distal sites remain... Read More about Cyclin A1 and P450 aromatase promote metastatic homing and growth of stem-like prostate cancer cells in the bone marrow.

Regulation of vascular endothelial growth factor in prostate cancer (2015)
Journal Article
de Brot, S., Ntekim, A., Cardenas, R., James, V., Allegrucci, C., Heery, D. M., …Mongan, N. P. (2015). Regulation of vascular endothelial growth factor in prostate cancer. Endocrine-Related Cancer, 22(3), Article R107-R123. https://doi.org/10.1530/ERC-15-0123

Prostate cancer (PCa) is the most common malignancy affecting men in the western world. Although radical prostatectomy and radiation therapy can successfully treat PCa in the majority of patients, up to ?30% will experience local recurrence or metast... Read More about Regulation of vascular endothelial growth factor in prostate cancer.