All Outputs (25)

Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms (2024)
Journal Article

Dairy slurry is a major source of environmental contamination with antimicrobial resistant genes and bacteria. We developed mathematical models and conducted on-farm research to explore the impact of wastewater flows and management practices on antim... Read More about Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms.

The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies (2024)
Journal Article

Viral metagenomics has fuelled a rapid change in our understanding of global viral diversity and ecology. Long-read sequencing and hybrid assembly approaches that combine long- and short-read technologies are now being widely implemented in bacterial... Read More about The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies.

Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh (2023)
Journal Article

Interactions between humans, animals, and the environment are considered critical foci for addressing antimicrobial resistance (AMR). However, One Health data on AMR in low- and middle-income countries are presently scarce. Using metagenomics, we inv... Read More about Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh.

The adaptability of the ion binding site by the Ag(I)/Cu(I) periplasmic chaperone SilF (2023)
Journal Article

The periplasmic chaperone SilF has been identified as part of an Ag(I) detoxification system in Gram negative bacteria. Sil proteins also bind Cu(I), but with reported weaker affinity, therefore leading to the designation of a specific detoxification... Read More about The adaptability of the ion binding site by the Ag(I)/Cu(I) periplasmic chaperone SilF.

Predicting bioactivity of antibiotic metabolites by molecular docking and dynamics (2023)
Journal Article

Antibiotics enter the environment through waste streams, where they can exert selective pressure for antimicrobial resistance in bacteria. However, many antibiotics are excreted as partly metabolized forms, or can be subject to partial breakdown in w... Read More about Predicting bioactivity of antibiotic metabolites by molecular docking and dynamics.

Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK (2023)
Journal Article

Globally, cephalosporin therapy failure is a serious problem for infection control. One causative agent of cephalosporin-resistant infections is multidrug-resistant (MDR) E. coli producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded... Read More about Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK.

Antimicrobial resistance in dairy slurry tanks: a critical point for measurement and control (2022)
Journal Article

Waste from dairy production is one of the largest sources of contamination from antimicrobial resistant bacteria (ARB) and genes (ARGs) in many parts of the world. However, studies to date do not provide necessary evidence to inform antimicrobial res... Read More about Antimicrobial resistance in dairy slurry tanks: a critical point for measurement and control.

Laboratory strains of Escherichia coli K-12: things are seldom what they seem (2022)
Journal Article

Escherichia coli K-12 was originally isolated 100 years ago and since then it has become an invaluable model organism and a cornerstone of molecular biology research. However, despite its pedigree, since its initial isolation E. coli K-12 has been re... Read More about Laboratory strains of Escherichia coli K-12: things are seldom what they seem.

Hybrid assembly of an agricultural slurry virome reveals a diverse and stable community with the potential to alter the metabolism and virulence of veterinary pathogens (2021)
Journal Article

Viruses are the most abundant biological entities on Earth, known to be crucial components of microbial ecosystems. However, there is little information on the viral community within agricultural waste. There are currently ~ 2.7 million dairy cattle... Read More about Hybrid assembly of an agricultural slurry virome reveals a diverse and stable community with the potential to alter the metabolism and virulence of veterinary pathogens.

Towards a general model for predicting minimal metal concentrations co-selecting for antibiotic resistance plasmids (2021)
Journal Article

© 2021 Elsevier Ltd Many antibiotic resistance genes co-occur with resistance genes for transition metals, such as copper, zinc, or mercury. In some environments, a positive correlation between high metal concentration and high abundance of antibioti... Read More about Towards a general model for predicting minimal metal concentrations co-selecting for antibiotic resistance plasmids.

A generalised model for generalised transduction: the importance of co-evolution and stochasticity in phage mediated antimicrobial resistance transfer (2020)
Journal Article

© FEMS 2020. Antimicrobial resistance is a major global challenge. Of particular concern are mobilizable elements that can transfer resistance genes between bacteria, leading to pathogens with new combinations of resistance. To date, mathematical mod... Read More about A generalised model for generalised transduction: the importance of co-evolution and stochasticity in phage mediated antimicrobial resistance transfer.

Removal of copper from cattle footbath wastewater with layered double hydroxide adsorbents as a route to antimicrobial resistance mitigation on dairy farms (2018)
Journal Article

Copper and zinc are routinely used in livestock antimicrobial footbaths in commercial farming. The footbath mix is a cost to farmers, and the disposal of spent footbath into slurry tanks leads to soil contamination, as well as the potential for antim... Read More about Removal of copper from cattle footbath wastewater with layered double hydroxide adsorbents as a route to antimicrobial resistance mitigation on dairy farms.

Anthropogenic environmental drivers of antimicrobial resistance in wildlife (2018)
Journal Article

The isolation of antimicrobial resistant bacteria (ARB) from wildlife living adjacent to humans has led to the suggestion that such antimicrobial resistance (AMR) is anthropogenically driven by exposure to antimicrobials and ARB. However, ARB have al... Read More about Anthropogenic environmental drivers of antimicrobial resistance in wildlife.

Comparative genomics of bacteriophage of the genus Seuratvirus (2017)
Journal Article

Despite being more abundant and having smaller genomes than their bacterial host, relatively few bacteriophages have had their genomes sequenced. Here, we isolated 14 bacteriophages from cattle slurry and performed de novo genome sequencing, assembly... Read More about Comparative genomics of bacteriophage of the genus Seuratvirus.

Bacterial resistance to arsenic protects against protist killing (2017)
Journal Article

Protists kill their bacterial prey using toxic metals such as copper. Here we hypothesize that the metalloid arsenic has a similar role. To test this hypothesis, we examined intracellular survival of Escherichia coli (E. coli) in the amoeba Dictyoste... Read More about Bacterial resistance to arsenic protects against protist killing.

Draft genome sequence of the bacteriophage vB_Eco_slurp01. (2016)
Journal Article

Bacteriophage vB_Eco_slurp01 was isolated from porcine feces using Escherichia coli MG1655 as a host. With a genome size of 348 kb, vB_Eco_slurp01 is one of the largest bacteriophages isolated to date.

SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance (2016)
Journal Article

Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an... Read More about SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance.

Mathematical modelling of antimicrobial resistance in agricultural waste highlights importance of gene transfer rate (2016)
Journal Article

Antimicrobial resistance is of global concern. Most antimicrobial use is in agriculture; manures and slurry are especially important because they contain a mix of bacteria, including potential pathogens, antimicrobial resistance genes and antimicrobi... Read More about Mathematical modelling of antimicrobial resistance in agricultural waste highlights importance of gene transfer rate.

Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm (2016)
Journal Article

Escherichia coli strains were isolated from a single dairy farm as a sentinel organism for the persistence of antibiotic resistance genes in the farm environment. Selective microbiological media were used to isolate 126 E. coli isolates from slurry a... Read More about Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm.

Draft genome sequences of 14 Escherichia coli phages isolated from cattle slurry (2015)
Journal Article

The diversity of bacteriophages in slurry from dairy cows remains largely unknown. Here, we report the draft genome sequences of 14 bacteriophages isolated from dairy cow slurry using Escherichia coli K-12 MG1655 as a host.