Delveen R. Ibrahim
Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK
Ibrahim, Delveen R.; Dodd, Christine E. R.; Stekel, Dov J.; Meshioye, Remilekun T.; Diggle, Mathew; Lister, Michelle; Hobman, Jon L.
Authors
Christine E. R. Dodd
DOV STEKEL DOV.STEKEL@NOTTINGHAM.AC.UK
Professor of Computational Biology
Remilekun T. Meshioye
Mathew Diggle
Michelle Lister
JON HOBMAN jon.hobman@nottingham.ac.uk
Associate Professor
Abstract
Globally, cephalosporin therapy failure is a serious problem for infection control. One causative agent of cephalosporin-resistant infections is multidrug-resistant (MDR) E. coli producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded AmpC (pAmpC) β-lactamases. We evaluated the occurrence of ESBL/pAmpC genetic determinants in phenotypically MDR E. coli isolated from clinical samples of blood, faeces, ear effusion, urine and sputum from a UK hospital. Phenotypic resistance profiling for 18 antibiotics (from seven classes) showed that 32/35 isolates were MDR, with resistance to 4–16 of the tested antibiotics. Of the isolates, 97.1% showed resistance to ampicillin, 71.4% showed resistance to co-amoxiclav, cefotaxime, ceftazidime and ceftiofur, and 68.5% showed resistance to cefquinome. blaCTX-M, blaTEM and blaOXA-1 genes were detected in 23, 13 and 12 strains, respectively, and Intl1 was detected in 17 isolates. The most common subtypes among the definite sequence types were CTX-M-15 (40%) and TEM-1 (75%). No E. coli isolates carried pAmpC genes. Significant correlations were seen between CTX-M carriage and cefotaxime, ceftiofur, aztreonam, ceftazidime and cefquinome resistance; between blaCTX-M, blaTEM and blaOXA-1 carriage and ciprofloxacin resistance; and between Intl1 carriage and trimethoprim/sulfamethoxazole resistance. Thus, MDR phenotypes may be conferred by a relatively small number of genes. The level and pattern of antibiotic resistance highlight the need for better antibiotic therapy guidelines, including reduced use and improved surveillance.
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 11, 2023 |
Online Publication Date | Jan 13, 2023 |
Publication Date | Jan 13, 2023 |
Deposit Date | Mar 17, 2023 |
Publicly Available Date | Mar 21, 2023 |
Journal | Antibiotics |
Electronic ISSN | 2079-6382 |
Publisher | MDPI AG |
Peer Reviewed | Peer Reviewed |
Volume | 12 |
Issue | 1 |
Article Number | 169 |
DOI | https://doi.org/10.3390/antibiotics12010169 |
Keywords | Escherichia coli; multidrug-resistant; cephalosporin-resistant; ESBL |
Public URL | https://nottingham-repository.worktribe.com/output/15940023 |
Publisher URL | https://www.mdpi.com/2079-6382/12/1/169 |
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Multidrug-Resistant ESBL
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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