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Comparative genomics of bacteriophage of the genus Seuratvirus

Sazinas, Pavelas; Redgwell, Tamsin; Rihtman, Branko; Grigonyte, Aurelija; Michniewski, Slawomir; Scanlan, David J.; Hobman, Jon L.; Millard, Andrew

Authors

Pavelas Sazinas

Tamsin Redgwell

Branko Rihtman

Aurelija Grigonyte

Slawomir Michniewski

David J. Scanlan

Jon L. Hobman

Andrew Millard



Abstract

Despite being more abundant and having smaller genomes than their bacterial host, relatively few bacteriophages have had their genomes sequenced. Here, we isolated 14 bacteriophages from cattle slurry and performed de novo genome sequencing, assembly, and annotation. The commonly used marker genes polB and terL showed these bacteriophages to be closely related to members of the genus Seuratvirus. We performed a core-gene analysis using the 14 new and four closely related genomes. A total of 58 core genes were identified, the majority of which has no known function. These genes were used to construct a core-gene phylogeny, the results of which confirmed the new isolates to be part of the genus Seuratvirus and expanded the number of species within this genus to four. All bacteriophages within the genus contained the genes queCDE encoding enzymes involved in queuosine biosynthesis. We suggest these genes are carried as a mechanism to modify DNA in order to protect these bacteriophages against host endonucleases.

Journal Article Type Article
Journal Genome Biology and Evolution
Electronic ISSN 1759-6653
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 10
Issue 1
APA6 Citation Sazinas, P., Redgwell, T., Rihtman, B., Grigonyte, A., Michniewski, S., Scanlan, D. J., …Millard, A. (in press). Comparative genomics of bacteriophage of the genus Seuratvirus. Genome Biology and Evolution, 10(1), https://doi.org/10.1093/gbe/evx275
DOI https://doi.org/10.1093/gbe/evx275
Keywords bacteriophage, genomics, evolution
Publisher URL https://academic.oup.com/gbe/article/10/1/72/4767719
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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