Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm

Ibrahim, Delveen R.; Dodd, Christine E. R.; Stekel, Dov J.; Ramsden, Stephen J.; Hobman, Jon L.

Abstract

Escherichia coli strains were isolated from a single dairy farm as a sentinel organism for the persistence of antibiotic resistance genes in the farm environment. Selective microbiological media were used to isolate 126 E. coli isolates from slurry and faeces samples from different farm areas. Antibiotic resistance profiling for 17 antibiotics (seven antibiotic classes), showed 57.9% of the isolates were resistant to between 3 and 15 antibiotics. The highest frequency of resistance was to ampicillin (56.3%), and the lowest to imipenem (1.6%), which appeared to be an unstable phenotype and was subsequently lost. Extended spectrum beta-lactamase resistance (ESBL) was detected in 53 isolates and blaCTX-M, blaTEM and blaOXA genes were detected by PCR in twelve, four and two strains, respectively. Phenotypically most isolates showing resistance to cephalosporins were AmpC rather than ESBL, a number of isolates having both activities. Phenotypic resistance patterns suggested co-acquisition of some resistance genes within subsets of the isolates. Genotyping using ERIC PCR demonstrated these were not clonal, and therefore co-resistance may be associated with mobile genetic elements. These data show a snapshot of diverse resistance genes present in the E. coli population reservoir, including resistance to historically used antibiotics as well as cephalosporins in contemporary use.

Citation

Ibrahim, D. R., Dodd, C. E. R., Stekel, D. J., Ramsden, S. J., & Hobman, J. L. (2016). Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm. FEMS Microbiology Ecology, 92(4), Article fiw013. https://doi.org/10.1093/femsec/fiw013

• Journal Article Type: Article
• Acceptance Date: Jan 23, 2016
• Online Publication Date: Feb 4, 2016
• Publication Date: 2016-04
• Deposit Date: Feb 11, 2016
• Publicly Available Date: Feb 11, 2016
• Journal: FEMS Microbiology Ecology
• Print ISSN: 0168-6496
• Electronic ISSN: 1574-6941
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 92
• Issue: 4
• Article Number: fiw013
• DOI: https://doi.org/10.1093/femsec/fiw013
• Keywords: Cattle Slurry, Escherichia Coli, Extended Spectrum Beta Lactamase, Multidrug Resistance
• Public URL: https://nottingham-repository.worktribe.com/output/777776
• Publisher URL: http://femsec.oxfordjournals.org/content/early/2016/02/04/femsec.fiw013.long
• Additional Information: This is a pre-copyedited, author-produced PDF of an article accepted for publication in FEMS Microbiology Ecology following peer review. Delveen R. Ibrahim, Christine E.R. Dodd, Dov J. Stekel, Stephen J. Ramsden, Jon L. Hobman Multi drug and extended spectrum beta-lactamase resistant Escherichia coli isolated from a dairy farm is available online at: https://academic.oup.com/femsec/article-lookup/doi/10.1093/femsec/fiw013
• Contract Date: Feb 11, 2016