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SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance

Asiani, Karishma R.; Williams, Huw Edward Llewelyn; Bird, Louise; Jenner, Matthew; Searle, Mark S.; Hobman, Jon L.; Scott, David J.; Soultanas, Panos

Authors

Karishma R. Asiani

Huw Edward Llewelyn Williams

Louise Bird

Matthew Jenner

Mark S. Searle

Jon L. Hobman

David J. Scott

Panos Soultanas panos.soultanas@nottingham.ac.uk



Abstract

Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an ATPase (SilP), an effluxer complex (SilCFBA) and a periplasmic chaperon of Ag+ (SilE). SilE is predicted to be intrinsically disordered. We tested this hypothesis using structural and biophysical studies and show that SilE is an intrinsically disordered protein in its free apo-form but folds to a compact structure upon optimal binding to six Ag+ ions in its holo-form. Sequence analyses and site-directed mutagenesis established the importance of histidine and methionine containing motifs for Ag+-binding, and identified a nucleation core that initiates Ag+-mediated folding of SilE. We conclude that SilE is a molecular sponge for absorbing metal ions.

Journal Article Type Article
Publication Date 2016-09
Journal Molecular Microbiology
Print ISSN 0950-382X
Electronic ISSN 1365-2958
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 101
Issue 5
Pages 731-742
APA6 Citation Asiani, K. R., Williams, H. E. L., Bird, L., Jenner, M., Searle, M. S., Hobman, J. L., …Soultanas, P. (2016). SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance. Molecular Microbiology, 101(5), 731-742. https://doi.org/10.1111/mmi.13399
DOI https://doi.org/10.1111/mmi.13399
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/mmi.13399/full
Related Public URLs http://www.ncbi.nlm.nih.gov/pubmed/27085056
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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