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SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance

Asiani, Karishma R.; Williams, Huw Edward Llewelyn; Bird, Louise; Jenner, Matthew; Searle, Mark S.; Hobman, Jon L.; Scott, David J.; Soultanas, Panos

SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance Thumbnail


Authors

Karishma R. Asiani

HUW WILLIAMS HUW.WILLIAMS@NOTTINGHAM.AC.UK
Senior Research Fellow

Louise Bird

Matthew Jenner

Mark S. Searle

JON HOBMAN jon.hobman@nottingham.ac.uk
Associate Professor

DAVID SCOTT DAVID.SCOTT@NOTTINGHAM.AC.UK
Associate Professor & Reader in Physical Biochemistry

PANOS SOULTANAS PANOS.SOULTANAS@NOTTINGHAM.AC.UK
Professor of Biological Chemistry



Abstract

Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an ATPase (SilP), an effluxer complex (SilCFBA) and a periplasmic chaperon of Ag+ (SilE). SilE is predicted to be intrinsically disordered. We tested this hypothesis using structural and biophysical studies and show that SilE is an intrinsically disordered protein in its free apo-form but folds to a compact structure upon optimal binding to six Ag+ ions in its holo-form. Sequence analyses and site-directed mutagenesis established the importance of histidine and methionine containing motifs for Ag+-binding, and identified a nucleation core that initiates Ag+-mediated folding of SilE. We conclude that SilE is a molecular sponge for absorbing metal ions.

Citation

Asiani, K. R., Williams, H. E. L., Bird, L., Jenner, M., Searle, M. S., Hobman, J. L., …Soultanas, P. (2016). SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance. Molecular Microbiology, 101(5), 731-742. https://doi.org/10.1111/mmi.13399

Journal Article Type Article
Acceptance Date Apr 15, 2016
Online Publication Date May 7, 2016
Publication Date Aug 26, 2016
Deposit Date Jun 24, 2016
Publicly Available Date Mar 28, 2024
Journal Molecular Microbiology
Print ISSN 0950-382X
Electronic ISSN 1365-2958
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 101
Issue 5
Pages 731-742
DOI https://doi.org/10.1111/mmi.13399
Public URL https://nottingham-repository.worktribe.com/output/790929
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/mmi.13399/full
Related Public URLs http://www.ncbi.nlm.nih.gov/pubmed/27085056

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