Karishma R. Asiani
SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance
Asiani, Karishma R.; Williams, Huw Edward Llewelyn; Bird, Louise; Jenner, Matthew; Searle, Mark S.; Hobman, Jon L.; Scott, David J.; Soultanas, Panos
Authors
HUW WILLIAMS HUW.WILLIAMS@NOTTINGHAM.AC.UK
Senior Research Fellow
Louise Bird
Matthew Jenner
Mark S. Searle
JON HOBMAN jon.hobman@nottingham.ac.uk
Associate Professor
DAVID SCOTT david.scott@nottingham.ac.uk
Associate Professor & Reader in Physical Biochemistry
PANOS SOULTANAS PANOS.SOULTANAS@NOTTINGHAM.AC.UK
Professor of Biological Chemistry
Abstract
Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an ATPase (SilP), an effluxer complex (SilCFBA) and a periplasmic chaperon of Ag+ (SilE). SilE is predicted to be intrinsically disordered. We tested this hypothesis using structural and biophysical studies and show that SilE is an intrinsically disordered protein in its free apo-form but folds to a compact structure upon optimal binding to six Ag+ ions in its holo-form. Sequence analyses and site-directed mutagenesis established the importance of histidine and methionine containing motifs for Ag+-binding, and identified a nucleation core that initiates Ag+-mediated folding of SilE. We conclude that SilE is a molecular sponge for absorbing metal ions.
Citation
Asiani, K. R., Williams, H. E. L., Bird, L., Jenner, M., Searle, M. S., Hobman, J. L., …Soultanas, P. (2016). SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance. Molecular Microbiology, 101(5), 731-742. https://doi.org/10.1111/mmi.13399
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Apr 15, 2016 |
| Online Publication Date | May 7, 2016 |
| Publication Date | Aug 26, 2016 |
| Deposit Date | Jun 24, 2016 |
| Publicly Available Date | Jun 24, 2016 |
| Journal | Molecular Microbiology |
| Print ISSN | 0950-382X |
| Electronic ISSN | 1365-2958 |
| Publisher | Wiley |
| Peer Reviewed | Peer Reviewed |
| Volume | 101 |
| Issue | 5 |
| Pages | 731-742 |
| DOI | https://doi.org/10.1111/mmi.13399 |
| Public URL | https://nottingham-repository.worktribe.com/output/790929 |
| Publisher URL | http://onlinelibrary.wiley.com/doi/10.1111/mmi.13399/full |
| Related Public URLs | http://www.ncbi.nlm.nih.gov/pubmed/27085056 |
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0