All Outputs (16)

Hepatitis C subtyping assay failure in UK patients born in sub-Saharan Africa: Implications for global treatment and elimination (2022)
Journal Article

Background andAims: The newly developed direct-acting antivirals have revolutionized the treatment of chronic hepatitis C virus (HCV), with cure rates as high as 98% in some cohorts. Although genome sequencing has demonstrated that some subtypes of H... Read More about Hepatitis C subtyping assay failure in UK patients born in sub-Saharan Africa: Implications for global treatment and elimination.

Simultaneous determination of HCV genotype and NS5B resistance associated substitutions using dried serum spots from São Paulo state, Brazil (2022)
Journal Article

Hepatitis C virus (HCV) is responsible for more than 180 million infections worldwide, and about 80 % of infections are reported in Low and Middle-income countries (LMICs). Therapy is based on the administration of interferon (INF), ribavirin (RBV) o... Read More about Simultaneous determination of HCV genotype and NS5B resistance associated substitutions using dried serum spots from São Paulo state, Brazil.

Nanopore sequencing from extraction-free direct PCR of dried serum spots for portable hepatitis B virus drug-resistance typing (2020)
Journal Article

© 2020 Background: Effective drug regimens for the treatment of hepatitis B virus (HBV) infections are essential to achieve the World Health Organisation commitment to eliminate viral hepatitis by 2030. Lamivudine (3TC) is widely used in countries wi... Read More about Nanopore sequencing from extraction-free direct PCR of dried serum spots for portable hepatitis B virus drug-resistance typing.

IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection (2019)
Journal Article

While adaptive immune responses against hepatitis C virus (HCV) infection have been studied in great detail, the role of innate immunity in protection against HCV infection and immune evasion is only partially understood. Interferon‐induced transmemb... Read More about IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection.

Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses (2019)
Journal Article

The liver-expressed pattern recognition receptors (PRRs) mannose binding lectin (MBL), ficolin-2 and ficolin-3 contribute to the innate immune response by activating complement. Binding of soluble ficolin-2 to viral pathogens can directly neutralize... Read More about Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses.

InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones (2018)
Book Chapter

This chapter describes how to generate chimeric molecular cassettes that are ready to receive PCR-amplified E1/E2 genes using new DNA cloning technology. The method is divided into three sections: (1) generation of a ΔCore-NS2 cassette based upon the... Read More about InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones.

How have retrovirus pseudotypes contributed to our understanding of viral entry? (2017)
Journal Article

Study of virus entry into host cells is important for understanding viral tropism and pathogenesis. Studying the entry of in vitro cultured viruses is not always practicable. Study of highly pathogenic viruses, viruses that do not grow in culture, an... Read More about How have retrovirus pseudotypes contributed to our understanding of viral entry?.

Novel functional hepatitis C virus glycoprotein isolates identified using an optimized viral pseudotype entry assay (2016)
Journal Article

Retrovirus pseudotypes are a highly tractable model used to study the entry pathways of enveloped viruses. This model has been extensively applied to the study of the hepatitis C virus (HCV) entry pathway, pre-clinical screening of antiviral antibodi... Read More about Novel functional hepatitis C virus glycoprotein isolates identified using an optimized viral pseudotype entry assay.

Flexible and rapid construction of viral chimeras applied to hepatitis C virus (2016)
Journal Article

A novel and broadly applicable strategy combining site directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using Hepatitis C Virus as an exemplar. Full-length HCV genomic cloning cassettes, which contained flex... Read More about Flexible and rapid construction of viral chimeras applied to hepatitis C virus.

Technical considerations for the generation of novel pseudotyped viruses (2016)
Journal Article

A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studie... Read More about Technical considerations for the generation of novel pseudotyped viruses.

Modulation of potassium channels inhibits bunyavirus infection (2015)
Journal Article

Bunyaviruses are considered to be emerging pathogens facilitated by the segmented nature of their genome that allows reassortment between different species to generate novel viruses with altered pathogenicity. Bunyaviruses are transmitted via a diver... Read More about Modulation of potassium channels inhibits bunyavirus infection.

Seroprevalence of Schmallenberg virus in the United Kingdom and the Republic of Ireland: 2011-2013 (2015)
Journal Article

© 2015 Elsevier B.V. Since its identification in late 2011, Schmallenberg virus (SBV) spread rapidly across Europe. Using archived samples from domestic ruminants collected between October 2011 and June 2013, the seroprevalence in the United Kingdom... Read More about Seroprevalence of Schmallenberg virus in the United Kingdom and the Republic of Ireland: 2011-2013.

Comparison of Schmallenberg virus antibody levels detected in milk and serum from individual cows (2015)
Journal Article

© Daly et al.; licensee BioMed Central. Background: Schmallenberg virus (SBV) is a recently emerged virus of ruminants in Europe. Enzyme-linked immunosorbent assays (ELISA) are commonly used to detect SBV-specific antibodies in bulk tank milk samples... Read More about Comparison of Schmallenberg virus antibody levels detected in milk and serum from individual cows.

Pseudotypes: your flexible friends (2014)
Journal Article

Pseudotype viruses: applications and troubleshooting' (EuroSciCon 2013), a 1-day conference held at Cineworld: The O2 (London, UK) on 2 October 2013, focused on the technique of pseudotyping enveloped viruses (for a review of the technique, see [1]).... Read More about Pseudotypes: your flexible friends.

Current progress with serological assays for exotic emerging / re-emerging viruses (2013)
Journal Article

Recent decades have witnessed an unprecedented rise in the outbreak occurrence of infectious and primarily zoonotic viruses. Contributing factors to this phenomenon include heightened global connectivity via air travel and international trade links,... Read More about Current progress with serological assays for exotic emerging / re-emerging viruses.

Troubleshooting methods for the generation of novel pseudotyped viruses
Journal Article

A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studie... Read More about Troubleshooting methods for the generation of novel pseudotyped viruses.