William L. Irving
Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses
Irving, William L.; Jalal, Paywast J.; Urbanowicz, Richard A.; Horncastle, Emma; Pathak, Monika; Goddard, Chun; Saeed, Amanj; Mason, Christopher P.; Ball, Jonathan K.; McClure, C. Patrick; King, Barnabas J.; Tarr, Alexander W.
Paywast J. Jalal
Richard A. Urbanowicz
Christopher P. Mason
Jonathan K. Ball
C. Patrick McClure
Barnabas J. King
Alexander W. Tarr
The liver-expressed pattern recognition receptors (PRRs) mannose binding lectin (MBL), ficolin-2 and ficolin-3 contribute to the innate immune response by activating complement. Binding of soluble ficolin-2 to viral pathogens can directly neutralize virus entry. We observed that the human hepatoma cell line HuH7.5, which is routinely used for the study of hepatotropic viruses, is deficient in expression of MBL, ficolin-2 and ficolin-3. We generated a cell line that expressed and secreted ficolin-2. This cell line (HuH7.5 [FCN2]) was more resistant to infection with hepatitis C virus (HCV), ebolavirus (EBOV) and vesicular stomatitis virus (VSV), but surprisingly was more susceptible to infection with rabies virus (RABV). Cell-to-cell spread of HCV was also inhibited in ficolin-2 expressing cells. This illustrates that ficolin-2 expression in hepatocytes contributes to innate resistance to virus infection, but some viruses might utilise ficolin-2 to facilitate entry.
|Journal Article Type||Article|
|Publication Date||Apr 1, 2019|
|Journal||Journal of Medical Microbiology|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Irving, W. L., Jalal, P. J., Urbanowicz, R. A., Horncastle, E., Pathak, M., Goddard, C., …Tarr, A. W. (2019). Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses. Journal of Medical Microbiology, 68(4), 642-648. https://doi.org/10.1099/jmm.0.000935|
|Keywords||Microbiology (medical); Microbiology; General Medicine|
Manuscript - Expression Of Human Ficolin-2 In Hepatocytes Confers Resist..