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Technical considerations for the generation of novel pseudotyped viruses

King, Barnabas; Temperton, Nigel; Grehan, Keith; Scott, Simon D.; Wright, Edward; Tarr, Alexander W.; Daly, Janet M.

Authors

Barnabas King

Nigel Temperton

Keith Grehan

Simon D. Scott

Edward Wright

Alexander W. Tarr

Janet M. Daly



Abstract

A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studies such as determining the cellular targets of the envelope protein as well as identification of potential antiviral compounds and measuring specific antibody responses. In this review, we describe the common methodologies employed to generate pseudotyped viruses (PVs) with a focus on approaches to improve the efficacy of PV generation.

Journal Article Type Article
Publication Date Jan 1, 2016
Journal Future Virology
Print ISSN 1746-0794
Electronic ISSN 1746-0808
Publisher Future Medicine
Peer Reviewed Not Peer Reviewed
Volume 11
Issue 1
APA6 Citation King, B., Temperton, N., Grehan, K., Scott, S. D., Wright, E., Tarr, A. W., & Daly, J. M. (2016). Technical considerations for the generation of novel pseudotyped viruses. Future Virology, 11(1), doi:10.2217/fvl.15.106
DOI https://doi.org/10.2217/fvl.15.106
Keywords Pseudotyped virus, pseudotypes, pseudoparticles, envelope glycoproteins
Publisher URL http://www.futuremedicine.com/doi/10.2217/fvl.15.106
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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