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Nanopore sequencing from extraction-free direct PCR of dried serum spots for portable hepatitis B virus drug-resistance typing

Astbury, Stuart; Nunes Soares, Marcia Maria Da Costa; Peprah, Emmanuel; King, Barnabas; Jardim, Ana Carolina Gomes; Shimizu, Jacqueline Farinha; Jalal, Paywast; Saeed, Chiman H.; Sabeer, Furat T.; Irving, William L.; Tarr, Alexander W.; McClure, Patrick

Authors

Marcia Maria Da Costa Nunes Soares

Emmanuel Peprah

Barnabas King

Ana Carolina Gomes Jardim

Jacqueline Farinha Shimizu

Paywast Jalal

Chiman H. Saeed

Furat T. Sabeer



Abstract

© 2020 Background: Effective drug regimens for the treatment of hepatitis B virus (HBV) infections are essential to achieve the World Health Organisation commitment to eliminate viral hepatitis by 2030. Lamivudine (3TC) is widely used in countries with high levels of chronic HBV, however resistance has been shown to occur in up to 50 % of individuals receiving continuous monotherapy for 4 years. Telbivudine (LdT) is now more commonly used in place of lamivudine but is ineffective against 3TC-resistant HBV. Genotyping and identification of resistanceassociated substitutions (RAS) is not practical in many locations. Objectives: A novel assay was designed to enable HBV genotyping and characterisation of resistance mutations directly from serum samples stored on filter paper, using Sanger and MinION sequencing. Study design: The assay was applied to a cohort of 30 samples stored on filter paper for several years with HBV viral loads ranging from 8.2 × 108 to 635 IU/mL. A set of 6 high-titre samples were used in a proof-of-principle study using the MinION sequencer. Results: The assay allowed determination of HBV genotype and elucidation of RAS down to 600 IU/mL using a 550bp amplicon. Sequencing of a 1.2 kb amplicon using a MinION sequencer gave results consistent with Sanger sequencing and allowed the identification of minor populations of variants. Conclusions: We present two approaches for reliable HBV sequencing and RAS identification using methods suitable for resource-limited environments. This is the first demonstration of extraction-free DNA sequencing direct from DSS using MinION and these workflows are adaptable to the investigation of other DNA viruses.

Journal Article Type Article
Publication Date 2020-08
Journal Journal of Clinical Virology
Print ISSN 1386-6532
Electronic ISSN 1873-5967
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 129
Article Number 104483
APA6 Citation Astbury, S., Nunes Soares, M. M. D. C., Peprah, E., King, B., Jardim, A. C. G., Shimizu, J. F., …McClure, P. (2020). Nanopore sequencing from extraction-free direct PCR of dried serum spots for portable hepatitis B virus drug-resistance typing. Journal of Clinical Virology, 129, https://doi.org/10.1016/j.jcv.2020.104483
DOI https://doi.org/10.1016/j.jcv.2020.104483
Keywords Virology; Infectious Diseases
Publisher URL https://www.sciencedirect.com/science/article/abs/pii/S1386653220302250
Additional Information This article is maintained by: Elsevier; Article Title: Nanopore sequencing from extraction-free direct PCR of dried serum spots for portable hepatitis B virus drug-resistance typing; Journal Title: Journal of Clinical Virology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.jcv.2020.104483; Content Type: article; Copyright: © 2020 Published by Elsevier B.V.
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