Skip to main content

Research Repository

Advanced Search

Simultaneous determination of HCV genotype and NS5B resistance associated substitutions using dried serum spots from São Paulo state, Brazil

Adeboyejo, Kazeem; Riquena Grosche, Victória; Pandeló José, Diego; Magalhães Ferreira, Giulia; Farinha Shimizu, Jacqueline; King, Barnabas J.; Tarr, Alexander W.; Costa Nunes Soares, Márcia Maria; Ball, Jonathan K.; McClure, C. Patrick; Gomes Jardim, Ana Carolina

Simultaneous determination of HCV genotype and NS5B resistance associated substitutions using dried serum spots from São Paulo state, Brazil Thumbnail


Authors

Kazeem Adeboyejo

Victória Riquena Grosche

Diego Pandeló José

Giulia Magalhães Ferreira

Jacqueline Farinha Shimizu

Barnabas J. King

Márcia Maria Costa Nunes Soares

JONATHAN BALL jonathan.ball@nottingham.ac.uk
Professor of Molecular Virology

Ana Carolina Gomes Jardim



Abstract

Hepatitis C virus (HCV) is responsible for more than 180 million infections worldwide, and about 80 % of infections are reported in Low and Middle-income countries (LMICs). Therapy is based on the administration of interferon (INF), ribavirin (RBV) or more recently Direct-Acting Antivirals (DAAs). However, amino acid substitutions associated with resistance (RAS) have been extensively described and can contribute to treatment failure, and diagnosis of RAS requires considerable infrastructure, not always locally available. Dried serum spots (DSS) sampling is an alternative specimen collection method, which embeds drops of serum onto filter paper to be transported by posting to a centralized laboratory. Here, we assessed feasibility of genotypic analysis of HCV from DSS in a cohort of 80 patients from São Paulo state Brazil. HCV RNA was detected on DSS specimens in 83 % of samples of HCV infected patients. HCV genotypes 1a, 1b, 2a, 2c and 3a were determined using the sequence of the palm domain of NS5B region, and RAS C316N/Y, Q309R and V321I were identified in HCV 1b samples. Concerning therapy outcome, 75 % of the patients who used INF +RBV as a previous protocol of treatment did not respond to DAAs, and 25 % were end-of-treatment responders. It suggests that therapy with INF plus RBV may contribute for non-response to a second therapeutic protocol with DAAs. One patient that presented RAS (V321I) was classified as non-responder, and combination of RAS C316N and Q309R does not necessarily imply in resistance to treatment in this cohort of patients. Data presented herein highlights the relevance of studying circulating variants for a better understanding of HCV variability and resistance to the therapy. Furthermore, the feasibility of carrying out genotyping and RAS phenotyping analysis by using DSS card for the potential of informing future treatment interventions could be relevant to overcome the limitations of processing samples in several location worldwide, especially in LMICs.

Citation

Adeboyejo, K., Riquena Grosche, V., Pandeló José, D., Magalhães Ferreira, G., Farinha Shimizu, J., King, B. J., …Gomes Jardim, A. C. (2022). Simultaneous determination of HCV genotype and NS5B resistance associated substitutions using dried serum spots from São Paulo state, Brazil. Access Microbiology, 4(3), Article 000326. https://doi.org/10.1099/acmi.0.000326

Journal Article Type Article
Acceptance Date Dec 30, 2021
Online Publication Date Mar 2, 2022
Publication Date Mar 2, 2022
Deposit Date Feb 14, 2022
Publicly Available Date Mar 28, 2024
Journal Access Microbiology
Electronic ISSN 2516-8290
Publisher Microbiology Society
Peer Reviewed Peer Reviewed
Volume 4
Issue 3
Article Number 000326
DOI https://doi.org/10.1099/acmi.0.000326
Public URL https://nottingham-repository.worktribe.com/output/7466808
Publisher URL https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000326