Troubleshooting methods for the generation of novel pseudotyped viruses

King, Barnabas; Temperton, Nigel; Grehan, Keith; Scott, Simon; Wright, Edward; Tarr, Alexander W.; Daly, Janet M.

Abstract

A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studies such as determining the cellular targets of the envelope protein of the virus as well as identification of potential antiviral compounds and measuring specific antibody responses. In this review, we describe the common methodologies employed to generate PVs, with a focus on approaches to improve the efficacy of PV generation.

Citation

King, B., Temperton, N., Grehan, K., Scott, S., Wright, E., Tarr, A. W., & Daly, J. M. Troubleshooting methods for the generation of novel pseudotyped viruses. Future Virology,

• Journal Article Type: Article
• Deposit Date: Oct 30, 2015
• Journal: Future Virology
• Print ISSN: 1746-0794
• Electronic ISSN: 1746-0808
• Publisher: Future Medicine
• Peer Reviewed: Not Peer Reviewed
• Keywords: Pseudotyped virus, pseudotypes, pseudoparticles, envelope glycoproteins
• Public URL: https://nottingham-repository.worktribe.com/output/980086
• Related Public URLs: http://www.futuremedicine.com/loi/fvl
• Additional Information: Article has been submitted for publication to Future Virology.