All Outputs (16)

Functional Characterization of 15 Novel Dense Granule Proteins in Toxoplasma gondii Using the CRISPR-Cas9 System (2022)
Journal Article
Zheng, X. N., Wang, J. L., Elsheikha, H. M., Wang, M., Zhang, Z. W., Sun, L. X., …Li, T. T. (2023). Functional Characterization of 15 Novel Dense Granule Proteins in Toxoplasma gondii Using the CRISPR-Cas9 System. Microbiology Spectrum, 11(1), https://doi.org/10.1128/spectrum.03078-22

The analysis of the subcellular localization and function of dense granule proteins (GRAs) is of central importance for the understanding of host-parasite interaction and pathogenesis of Toxoplasma gondii infection. Here, we identified 15 novel GRAs... Read More about Functional Characterization of 15 Novel Dense Granule Proteins in Toxoplasma gondii Using the CRISPR-Cas9 System.

The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii (2022)
Journal Article
Wang, J., Li, T., Elsheikha, H. M., Liang, Q., Zhang, Z., Wang, M., …Zhu, X. (2022). The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii. Nature Communications, 13(1), Article 7560. https://doi.org/10.1038/s41467-022-35267-5

Phenotypic switching between tachyzoite and bradyzoite is the fundamental mechanism underpinning the pathogenicity and adaptability of the protozoan parasite Toxoplasma gondii. Although accumulation of cytoplasmic starch granules is a hallmark of the... Read More about The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii.

Effect of deleting four Toxoplasma gondii calcium-binding EGF domain-containing proteins on parasite replication and virulence (2022)
Journal Article
Wang, X., Li, T., Elsheikha, H. M., Zheng, X., Zhao, D., Wang, J., …Zhu, X. (2023). Effect of deleting four Toxoplasma gondii calcium-binding EGF domain-containing proteins on parasite replication and virulence. Parasitology Research, 122, 441-450. https://doi.org/10.1007/s00436-022-07739-6

Several calcium-binding proteins including calcium-dependent protein kinases play important roles in several facets of the intracellular infection cycle of the apicomplexan protozoan parasite Toxoplasma gondii. However, the role of the calcium-bindin... Read More about Effect of deleting four Toxoplasma gondii calcium-binding EGF domain-containing proteins on parasite replication and virulence.

Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii (2022)
Journal Article
Fakae, L. B., Harun, M. S., Ting, D. S. J., Dua, H. S., Cave, G. W., Zhu, X. Q., …Elsheikha, H. M. (2023). Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii. Acta Tropica, 237, Article 106729. https://doi.org/10.1016/j.actatropica.2022.106729

We examined the anti-acanthamoebic efficacy of green tea Camellia sinensis solvent extract (SE) or its chemical constituents against Acanthamoeba castellanii by using anti-trophozoite, anti-encystation, and anti-excystation assays. C. sinensis SE (62... Read More about Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii.

Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity (2022)
Journal Article
Helal, M. A., Abdel-Gawad, A. M., Kandil, O. M., Khalifa, M. M., Morrison, A. A., Bartley, D. J., …Elsheikha, H. M. (2022). Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity. Pharmaceutics, 14(10), Article 2030. https://doi.org/10.3390/pharmaceutics14102030

In this study, poly (lactic-co-glycolic) acid (PLGA) particles were synthesized and coated with chitosan. Three essential oil (EO) components (eugenol, linalool, and geraniol) were entrapped inside these PLGA particles by using the continuous flow-fo... Read More about Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity.

Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection (2022)
Journal Article
Li, H. Y., Zou, Y., Elsheikha, H. M., Xu, Y., Cai, L., Xie, S. C., …Zheng, W. B. (2022). Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection. Frontiers in Cellular and Infection Microbiology, 12, Article 890589. https://doi.org/10.3389/fcimb.2022.890589

A global lipidomic analysis using liquid chromatography–tandem mass spectrometry was performed on the liver of beagle dogs infected with Toxocara canis to profile hepatic lipid species at 12h post-infection (hpi), 24 hpi, and 36 days post-infection (... Read More about Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection.

Terrestrial runoff influences the transport and contamination levels of Toxoplasma gondii in marine organisms (2022)
Journal Article
Li, M. Y., Kang, Y. H., Sun, W. C., Hao, Z. P., Elsheikha, H. M., & Cong, W. (2022). Terrestrial runoff influences the transport and contamination levels of Toxoplasma gondii in marine organisms. Science of the Total Environment, 851, Article 158168. https://doi.org/10.1016/j.scitotenv.2022.158168

There is a growing concern regarding the potential adverse impact of Toxoplasma gondii contamination of the marine environment on marine wildlife and public health. Terrestrial runoff is a significant route for dissemination of T. gondii oocysts from... Read More about Terrestrial runoff influences the transport and contamination levels of Toxoplasma gondii in marine organisms.

Perception of UK companion animal veterinarians on risk assessment based parasite control (2022)
Journal Article
Bagster, A., & Elsheikha, H. (2022). Perception of UK companion animal veterinarians on risk assessment based parasite control. Veterinary parasitology (Amsterdam), 34, Article 100774. https://doi.org/10.1016/j.vprsr.2022.100774

Parasites can pose a risk to companion animals and potentially their owners. Current parasiticide use is possibly impacting the environment, increasing adverse reaction and resistance risk. As such parasiticides should be dispensed by the veterinary... Read More about Perception of UK companion animal veterinarians on risk assessment based parasite control.

RNA sequencing reveals dynamic expression of spleen lncRNAs and mRNAs in Beagle dogs infected by Toxocara canis (2022)
Journal Article
Xu, Y., Zheng, W. B., Li, H. Y., Cai, L., Zou, Y., Xie, S. C., …Elsheikha, H. M. (2022). RNA sequencing reveals dynamic expression of spleen lncRNAs and mRNAs in Beagle dogs infected by Toxocara canis. Parasites and Vectors, 15(1), Article 279. https://doi.org/10.1186/s13071-022-05380-x

Background: Toxocara canis is a cosmopolitan parasite with a significant adverse impact on the health of humans and animals. The spleen is a major immune organ that plays essential roles in protecting the host against various infections. However, its... Read More about RNA sequencing reveals dynamic expression of spleen lncRNAs and mRNAs in Beagle dogs infected by Toxocara canis.

Diagnosis and Management of Acanthamoeba Keratitis: A Continental Approach (2022)
Journal Article
Bouten, M., & Elsheikha, H. M. (2022). Diagnosis and Management of Acanthamoeba Keratitis: A Continental Approach. Parasitologia, 2(3), 167-197. https://doi.org/10.3390/parasitologia2030016

Acanthamoeba keratitis (AK) is a potentially blinding infection caused by protozoa found worldwide. The topical application of biguanides and diamidines is the most common anti-amoebic treatment for AK. In this study, we hypothesized that geographica... Read More about Diagnosis and Management of Acanthamoeba Keratitis: A Continental Approach.

Human pediculosis, a global public health problem (2022)
Journal Article
Fu, Y., Yao, C., Deng, Y., Elsheikha, H. M., Shao, R., Zhu, X., & Liu, G. (2022). Human pediculosis, a global public health problem. Infectious Diseases of Poverty, 11(1), Article 58. https://doi.org/10.1186/s40249-022-00986-w

Background: Human pediculosis is caused by hematophagous lice, which are transmitted between individuals via direct and/or indirect contact. Despite the public health importance of louse infestation, information concerning the global burden of pedicu... Read More about Human pediculosis, a global public health problem.

Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds (2022)
Journal Article
Nie, L., Liang, Q., Wang, M., Du, R., Zhang, M., Elsheikha, H. M., & Zhu, X. (2022). Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds. PLoS Neglected Tropical Diseases, 16(5), Article e0010431. https://doi.org/10.1371/journal.pntd.0010431

Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages... Read More about Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds.

Illuminating Host-Parasite Interaction at the Cellular and Subcellular Levels with Infrared Microspectroscopy (2022)
Journal Article
Elsheikha, H. M., Al-Sandaqchi, A. T., Harun, M. S., Winterton, F., Altharawi, A., Elsaied, N. A., …Chan, K. L. A. (2022). Illuminating Host-Parasite Interaction at the Cellular and Subcellular Levels with Infrared Microspectroscopy. Cells, 11(5), Article 811. https://doi.org/10.3390/cells11050811

Toxoplasma gondii (T. gondii) is an opportunistic protozoan that can cause brain infection and other serious health consequences in immuno-compromised individuals. This parasite has a remarkable ability to cross biological barriers and exploit the ho... Read More about Illuminating Host-Parasite Interaction at the Cellular and Subcellular Levels with Infrared Microspectroscopy.

A systematic review, meta‐analysis and meta‐regression of the global prevalence of Toxoplasma gondii infection in wild marine mammals and associations with epidemiological variables (2022)
Journal Article
Li, M., Gao, X., Ma, J., Elsheikha, H. M., & Cong, W. (2022). A systematic review, meta‐analysis and meta‐regression of the global prevalence of Toxoplasma gondii infection in wild marine mammals and associations with epidemiological variables. Transboundary and Emerging Diseases, 69(5), e1213-e1230. https://doi.org/10.1111/tbed.14493

Toxoplasma gondii infection in wild marine mammals is a growing problem and is associated with adverse impacts on marine animal and public health. This systematic review, meta-analysis and meta-regression estimates the global prevalence of T. gondii... Read More about A systematic review, meta‐analysis and meta‐regression of the global prevalence of Toxoplasma gondii infection in wild marine mammals and associations with epidemiological variables.

Functional Characterization of 17 Protein Serine/Threonine Phosphatases in Toxoplasma gondii Using CRISPR-Cas9 System (2022)
Journal Article
Liang, Q. L., Nie, L. B., Li, T. T., Elsheikha, H. M., Sun, L. X., Zhang, Z. W., …Wang, J. L. (2022). Functional Characterization of 17 Protein Serine/Threonine Phosphatases in Toxoplasma gondii Using CRISPR-Cas9 System. Frontiers in Cell and Developmental Biology, 9, Article 738794. https://doi.org/10.3389/fcell.2021.738794

Protein serine/threonine phosphatases (PSPs), found in various plants and protozoa, are involved in the regulation of various biological processes. However, very little is known about the role of PSPs in the pathogenicity of the apicomplexan protozoa... Read More about Functional Characterization of 17 Protein Serine/Threonine Phosphatases in Toxoplasma gondii Using CRISPR-Cas9 System.

Temporal transcriptomic changes in long non-coding RNAs and messenger RNAs involved in the host immune and metabolic response during Toxoplasma gondii lytic cycle (2022)
Journal Article
Wang, S. S., Zhou, C. X., Elsheikha, H. M., He, J. J., Zou, F. C., Zheng, W. B., …Zhao, G. H. (2022). Temporal transcriptomic changes in long non-coding RNAs and messenger RNAs involved in the host immune and metabolic response during Toxoplasma gondii lytic cycle. Parasites and Vectors, 15, Article 22. https://doi.org/10.1186/s13071-021-05140-3

Background: Long non-coding RNAs (lncRNAs) are important regulators of various biological and pathological processes, in particular the inflammatory response by modulating the transcriptional control of inflammatory genes. However, the role of lncRNA... Read More about Temporal transcriptomic changes in long non-coding RNAs and messenger RNAs involved in the host immune and metabolic response during Toxoplasma gondii lytic cycle.