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Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds

Nie, Lan-Bi; Liang, Qin-Li; Wang, Meng; Du, Rui; Zhang, Meng-Yuan; Elsheikha, Hany M.; Zhu, Xing-Quan

Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds Thumbnail


Authors

Lan-Bi Nie

Qin-Li Liang

Meng Wang

Rui Du

Meng-Yuan Zhang

Xing-Quan Zhu



Contributors

Javier Sotillo
Editor

Abstract

Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages. Yet, insights into such variations are needed to understand the extent to which lysine malonylation contributes to the differences in the virulence and repertoire of virulence factors between T. gondii genotypes. In this study, we profiled lysine malonylation in T. gondii using quantitative liquid chromatography tandem mass spectrometry (LC-MS/MS) and immuno-affinity purification. This analysis was performed on three T. gondii strains with distinctive pathogenicity in mice, including RH strain (type I), PRU strain (type II), and VEG strain (type III). In total, 111 differentially malonylated proteins and 152 sites were upregulated, and 17 proteins and 17 sites were downregulated in RH strain versus PRU strain; 50 proteins and 59 sites were upregu-lated, 50 proteins and 53 sites were downregulated in RH strain versus VEG strain; and 72 proteins and 90 sites were upregulated, and 7 proteins and 8 sites were downregulated in VEG strain versus PRU strain. Differentially malonylated proteins were involved in key processes , such as those mediating the regulation of protein metabolism, stress response, gly-colysis, and actin cytoskeleton. These results reveal an association between lysine malonylation and intra-species virulence differences in T. gondii and offer a new resource for elucidating the contribution of lysine malonylation to energy metabolism and virulence in T. gondii.

Citation

Nie, L., Liang, Q., Wang, M., Du, R., Zhang, M., Elsheikha, H. M., & Zhu, X. (2022). Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds. PLoS Neglected Tropical Diseases, 16(5), Article e0010431. https://doi.org/10.1371/journal.pntd.0010431

Journal Article Type Article
Acceptance Date Apr 18, 2022
Online Publication Date May 16, 2022
Publication Date May 16, 2022
Deposit Date May 28, 2022
Publicly Available Date Jun 1, 2022
Journal PLoS Neglected Tropical Diseases
Electronic ISSN 1935-2735
Publisher Public Library of Science (PLoS)
Peer Reviewed Peer Reviewed
Volume 16
Issue 5
Article Number e0010431
DOI https://doi.org/10.1371/journal.pntd.0010431
Keywords Infectious Diseases; Public Health, Environmental and Occupational Health
Public URL https://nottingham-repository.worktribe.com/output/8231487
Publisher URL https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0010431

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