Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Alaa T. Al-Sandaqchi
Mohammad S.R. Harun
Francesca Winterton
Ali Altharawi
Nashwa A. Elsaied
Dr CARL STEVENSON carl.stevenson@nottingham.ac.uk
Associate Professor
William MacNaughtan
John G.M. Mina
Paul W. Denny
Gianfelice Cinque
Ka Lung Andrew Chan
Toxoplasma gondii (T. gondii) is an opportunistic protozoan that can cause brain infection and other serious health consequences in immuno-compromised individuals. This parasite has a remarkable ability to cross biological barriers and exploit the host cell microenvironment to support its own survival and growth. Recent advances in label-free spectroscopic imaging techniques have made it possible to study biological systems at a high spatial resolution. In this study, we used conventional Fourier-transform infrared (FTIR) microspectroscopy and synchrotron-based FTIR microspectroscopy to analyze the chemical changes that are associated with infection of human brain microvascular endothelial cells (hBMECs) by T. gondii (RH) tachyzoites. Both FTIR microspectroscopic methods showed utility in revealing the chemical alterations in the infected hBMECs. Using a ZnS hemisphere device, to increase the numerical aperture, and the synchrotron source to increase the brightness, we obtained spatially resolved spectra from within a single cell. The spectra extracted from the nucleus and cytosol containing the tachyzoites were clearly distinguished. RNA sequencing analysis of T. gondii-infected and uninfected hBMECs revealed significant changes in the expression of host cell genes and pathways in response to T. gondii infection. These FTIR spectroscopic and transcriptomic findings provide significant insight into the molecular changes that occur in hBMECs during T. gondii infection.
Elsheikha, H. M., Al-Sandaqchi, A. T., Harun, M. S., Winterton, F., Altharawi, A., Elsaied, N. A., …Chan, K. L. A. (2022). Illuminating Host-Parasite Interaction at the Cellular and Subcellular Levels with Infrared Microspectroscopy. Cells, 11(5), Article 811. https://doi.org/10.3390/cells11050811
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 22, 2022 |
Online Publication Date | Feb 25, 2022 |
Publication Date | Mar 1, 2022 |
Deposit Date | Mar 26, 2022 |
Publicly Available Date | Mar 29, 2022 |
Journal | Cells |
Electronic ISSN | 2073-4409 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 11 |
Issue | 5 |
Article Number | 811 |
DOI | https://doi.org/10.3390/cells11050811 |
Keywords | General Medicine |
Public URL | https://nottingham-repository.worktribe.com/output/7537723 |
Publisher URL | https://www.mdpi.com/2073-4409/11/5/811 |
Elsheikha Et Al 2022
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