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Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection

Li, Hao Yu; Zou, Yang; Elsheikha, Hany M.; Xu, Yue; Cai, Lang; Xie, Shi Chen; Zhu, Xing Quan; Zheng, Wen Bin

Authors

Hao Yu Li

Yang Zou

Yue Xu

Lang Cai

Shi Chen Xie

Xing Quan Zhu

Wen Bin Zheng



Abstract

A global lipidomic analysis using liquid chromatography–tandem mass spectrometry was performed on the liver of beagle dogs infected with Toxocara canis to profile hepatic lipid species at 12h post-infection (hpi), 24 hpi, and 36 days post-infection (dpi). This analysis identified six categories and 42 subclasses of lipids, including 173, 64, and 116 differentially abundant lipid species at 12 hpi, 24 hpi, and 36 dpi, respectively. Many of the identified lysophospholipids, such as lysophosphatidylglycerol, lysophosphatidylserine, and lysophosphatidylcholine, may contribute to the migration and development of T. canis during the early infection stage. Pathway analysis revealed significant alterations of several immune-inflammatory pathways, such as the B-cell receptor signaling pathway, the NF-kappa B signaling pathway, and the C-type lectin receptor signaling pathway at 12 and 24 hpi. These findings demonstrate the value of lipidomic profiling in revealing the extent of changes in the composition and abundance of hepatic lipidome caused by T. canis infection and their relevance to the pathophysiology of toxocariasis in beagle dogs.

Journal Article Type Article
Acceptance Date Aug 18, 2022
Online Publication Date Sep 13, 2022
Publication Date Sep 13, 2022
Deposit Date Sep 13, 2022
Publicly Available Date Sep 15, 2022
Journal Frontiers in Cellular and Infection Microbiology
Electronic ISSN 2235-2988
Publisher Frontiers Media SA
Peer Reviewed Peer Reviewed
Volume 12
Article Number 890589
DOI https://doi.org/10.3389/fcimb.2022.890589
Keywords Cellular and Infection Microbiology, Toxocara canis, toxocariasis, beagle dog, liver, lipidomics
Public URL https://nottingham-repository.worktribe.com/output/11194638
Publisher URL https://www.frontiersin.org/articles/10.3389/fcimb.2022.890589/full