All Outputs (26)

Disruption of Epithelial Barriers for Molecular Delivery Enhancement or Extraction of Extracellular Fluids (2024)
Patent
Webb, K., Moradi, E., & Foss, A. (2024). Disruption of Epithelial Barriers for Molecular Delivery Enhancement or Extraction of Extracellular Fluids. WO2024009101A1. UK

The invention provides a method of delivering a payload molecule across an epithelial tissue barrier, the method comprising: applying the payload molecule to the epithelial tissue barrier, and additionally applying an agent to the epithelial tissue b... Read More about Disruption of Epithelial Barriers for Molecular Delivery Enhancement or Extraction of Extracellular Fluids.

Investigation of Exhaust Particles on Different TEM Grids: a Comparison between Graphene Oxide and Silicon Nitride Grids (2023)
Presentation / Conference Contribution
Lagana, S., Akifjevs, R., Rocca, A. L., Cairns, A., Fay, M. W., & Webb, K. F. (2023, August). Investigation of Exhaust Particles on Different TEM Grids: a Comparison between Graphene Oxide and Silicon Nitride Grids. Presented at 2023 JSAE/SAE Powertrains, Energy and Lubricants International Meeting, Kyoto, Japan

Two different TEM (Transmission Electron Microscopy) grids - graphene oxide (GO) and silicon nitride (SiN) - were used to capture the particulates emitted with the exhaust of a modern 1.0 L GDI (Gasoline Direct Injection) engine. One speed-load condi... Read More about Investigation of Exhaust Particles on Different TEM Grids: a Comparison between Graphene Oxide and Silicon Nitride Grids.

The Modular µSiM: A Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro (2022)
Journal Article
McCloskey, M. C., Kasap, P., Ahmad, S. D., Su, S., Chen, K., Mansouri, M., Ramesh, N., Nishihara, H., Belyaev, Y., Abhyankar, V. V., Begolo, S., Singer, B. H., Webb, K. F., Kurabayashi, K., Flax, J., Waugh, R. E., Engelhardt, B., & McGrath, J. L. (2022). The Modular µSiM: A Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro. Advanced Healthcare Materials, 11(18), Article 2200804. https://doi.org/10.1002/adhm.202200804

Advanced in vitro tissue chip models can reduce and replace animal experimentation and may eventually support "on-chip" clinical trials. To realize this potential, however, tissue chip platforms must be both mass-produced and reconfigurable to allow... Read More about The Modular µSiM: A Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro.

Non-invasive isotope-based hydrodynamic imaging in plant roots at cellular resolution (2022)
Presentation / Conference Contribution
Couvreur, V., Pascut, F. C., Dietrich, D., Leftley, N., Reyt, G., Boursiac, Y., Calvo-Polanco, M., Casimiro, I., Maurel, C., Salt, D. E., Draye, X., Wells, D. M., Bennett, M. J., & Webb, K. F. (2022, May). Non-invasive isotope-based hydrodynamic imaging in plant roots at cellular resolution. Presented at EGU General Assembly 2022, Vienna, Austria and online

A key impediment to studying water-related mechanisms in plants is the inability to noninvasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modellin... Read More about Non-invasive isotope-based hydrodynamic imaging in plant roots at cellular resolution.

Non-invasive hydrodynamic imaging in plant roots at cellular resolution (2021)
Journal Article
Pascut, F. C., Couvreur, V., Dietrich, D., Leftley, N., Reyt, G., Boursiac, Y., Calvo-Polanco, M., Casimiro, I., Maurel, C., Salt, D. E., Draye, X., Wells, D. M., Bennett, M. J., & Webb, K. F. (2021). Non-invasive hydrodynamic imaging in plant roots at cellular resolution. Nature Communications, 12, Article 4682. https://doi.org/10.1038/s41467-021-24913-z

A key impediment to studying water-related mechanisms in plants is the inability to non-invasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelli... Read More about Non-invasive hydrodynamic imaging in plant roots at cellular resolution.

Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells (2020)
Journal Article
Pérez-Cota, F., Fuentes-Domínguez, R., La Cavera III, S., Hardiman, W., Yao, M., Setchfield, K., Moradi, E., Naznin, S., Wright, A., Webb, K. F., Huett, A., Friel, C., Sottile, V., Elsheikha, H. M., Smith, R. J., & Clark, M. (2020). Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells. Journal of Applied Physics, 128(16), Article 160902. https://doi.org/10.1063/5.0023744

© 2020 Author(s). Characterization of the elasticity of biological cells is growing as a new way to gain insight into cell biology. Cell mechanics are related to most aspects of cellular behavior, and applications in research and medicine are broad.... Read More about Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells.

Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro (2020)
Journal Article
Harun, M., Marsh, V., Elsaied, N. A., Webb, K. F., & Elsheikha, H. M. (2020). Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro. Brain Research, 1746, Article 147002. https://doi.org/10.1016/j.brainres.2020.147002

Toxoplasma gondii can cause parasitic encephalitis, a life-threatening infection that predominately occurs in immunocompromised individuals. T. gondii has the ability to invade the brain, but the mechanisms by which this parasite crosses the blood–br... Read More about Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro.

Surface plasmon resonance imaging of excitable cells (2019)
Journal Article
Howe, C. L., Webb, K. F., Abayzeed, S. A., Anderson, D. J., Denning, C., & Russell, N. A. (2019). Surface plasmon resonance imaging of excitable cells. Journal of Physics D: Applied Physics, 52(10), Article 104001. https://doi.org/10.1088/1361-6463/aaf849

Surface plasmons (SPs) are surface charge density oscillations occuring at a metal/dieletric interface and are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and... Read More about Surface plasmon resonance imaging of excitable cells.

Surface Plasmon Resonance Imaging of Excitable Cells (2018)
Preprint / Working Paper
Howe, C. L., Webb, K. F., Abayzeed, S. A., Anderson, D. J., Denning, C., & Russell, N. A. Surface Plasmon Resonance Imaging of Excitable Cells

Surface plasmons are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and biological assays. In these systems, a surface plasmon resonance (SPR)-based sensor detect... Read More about Surface Plasmon Resonance Imaging of Excitable Cells.

Non destructive evaluation of biological cells (2018)
Presentation / Conference Contribution
Pérez-Cota, F., Smith, R. J., Moradi, E., Webb, K. F., & Clark, M. Non destructive evaluation of biological cells

© 2019 Author(s). Regenerative medicine promises to be the next revolution in health care. This technology, which will be the first systematic manufacturing of biological parts for human consumption, requires non destructive evaluatioin (NDE) thechni... Read More about Non destructive evaluation of biological cells.

Sensitive detection of voltage transients using differential intensity surface plasmon resonance system (2017)
Journal Article
Abayzeed, S. A., Smith, R. J., Webb, K. F., Somekh, M. G., & See, C. W. (2017). Sensitive detection of voltage transients using differential intensity surface plasmon resonance system. Optics Express, 25(25), 31552-31567. https://doi.org/10.1364/OE.25.031552

This paper describes theoretical and experimental study of the fundamentals of using surface plasmon resonance (SPR) for label-free detection of voltage. Plasmonic voltage sensing relies on the capacitive properties of metal-electrolyte interface tha... Read More about Sensitive detection of voltage transients using differential intensity surface plasmon resonance system.

Responsivity of the differential-intensity surface plasmon resonance instrument (2016)
Journal Article
Abayzeed, S. A., Smith, R. J., Webb, K. F., Somekh, M. G., & See, C. W. (2016). Responsivity of the differential-intensity surface plasmon resonance instrument. Sensors and Actuators B: Chemical, 235, 627-635. https://doi.org/10.1016/j.snb.2016.05.117

Surface plasmon resonance is used for the sensitive measurement of minute concentrations of bio-analytes and probing of electrochemical processes. Typical refractive index sensitivity, for the intensity approach, is around 10−6 refractive index units... Read More about Responsivity of the differential-intensity surface plasmon resonance instrument.

Cell imaging using sub-optical wavelength ultrasonics (2015)
Presentation / Conference Contribution
Clark, M., Marques, L., Moradi, E., Perez-Cota, F., Smith, R. J., & Webb, K. F. (2015, November). Cell imaging using sub-optical wavelength ultrasonics. Presented at Asia Communications and Photonics Conference, ACPC 2015, Hong Kong

At GHz frequencies the wavelength of ultrasound becomes comparable or smaller than the wavelength of light and so offers an intriguing way to image at the small scale. In this paper we report on new developments in pico-second laser ultrasonics that... Read More about Cell imaging using sub-optical wavelength ultrasonics.

Sub-100 nm resolution microscopy based on proximity projection grating scheme (2015)
Journal Article
Hu, F., Somekh, M. G., Albutt, D. J., Webb, K., Moradi, E., & See, C. W. (2015). Sub-100 nm resolution microscopy based on proximity projection grating scheme. Scientific Reports, 5, Article 8589. https://doi.org/10.1038/srep08589

Structured illumination microscopy (SIM) has been widely used in life science imaging applications. The maximum resolution improvement of SIM, compared to conventional bright field system is a factor of 2. Here we present an approach to structured il... Read More about Sub-100 nm resolution microscopy based on proximity projection grating scheme.

Sound of nano (2013)
Presentation / Conference Contribution
Aylott, J., Clark, M., Marques, L., Perez-Cota, F., Smith, R. J., & Webb, K. F. Sound of nano. Presented at SPIE Micro+Nano Materials, Devices, and Applications, Melbourne, Victoria, Australia

Ultrasound is widely used for imaging, measurement and diagnostics in the MHz region and is perhaps most familiar as a medical or non-destructive imaging or measurement tool. In the MHz frequency range the wavelength is typically measured in microns... Read More about Sound of nano.

Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage (2012)
Journal Article
Gunning, S. J., Chung, K. K., Donaldson, P. J., & Webb, K. F. (2012). Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage. American Journal of Physiology - Cell Physiology, 303(12), C1252-C1259. https://doi.org/10.1152/ajpcell.00237.2012

The initiation of lens cataract has long been associated with the development of a membrane "leak" in lens fiber cells that depolarizes the lens intracellular potential and elevates intracellular Na+ and Ca2+ concentrations. It has been proposed that... Read More about Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage.

Multimodal imaging of fluid transport in living epithelial sheets (2011)
Presentation / Conference Contribution
Webb, K. F., Zhang, J., & Somekh, M. (2011, December). Multimodal imaging of fluid transport in living epithelial sheets. Presented at 2011 Functional Optical Imaging, FOI 2011, Ningbo, China

Detailed knowledge on the biophysical mechanisms and local intercompartmental dynamics by which fluid and solute transport is achieved and regulated at the cellular and subcellular level is lacking. In this paper, cultures of immortalised human retin... Read More about Multimodal imaging of fluid transport in living epithelial sheets.

Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation (2011)
Journal Article
Mendoza-Naranjo, A., Bouma, G., Pereda, C., Ramiŕez, M., Webb, K. F., Tittarelli, A., Loṕez, M. N., Kalergis, A. M., Thrasher, A. J., Becker, D. L., & Salazar-Onfray, F. (2011). Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation. Journal of Immunology, 187(6), 3121-3132. https://doi.org/10.4049/jimmunol.1100378

Gap junction (GJ) mediates intercellular communication through linked hemichannels from each of two adjacent cells. Using human and mouse models, we show that connexin 43 (Cx43), the main GJ protein in the immune system, was recruited to the immunolo... Read More about Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation.

Ionic Permeability and Currents in the Lens (2010)
Book Chapter
Donaldson, P. J., & Webb, K. F. (2010). Ionic Permeability and Currents in the Lens. In Encyclopedia of the Eye (477-486). https://doi.org/10.1016/B978-0-12-374203-2.00032-4

© 2010 Elsevier Ltd. All rights reserved. It has been proposed that in the absence of a blood supply the lens operates an internal microcirculation system. In this system spatially distinct differences in the membrane transport properties of the lens... Read More about Ionic Permeability and Currents in the Lens.

Whole-cell patch clamping of isolated fiber cells confirms that spatially distinct Cl- influx and efflux pathways exist in the cortex of the rat lens (2009)
Journal Article
Webb, K. F., & Donaldson, P. J. (2009). Whole-cell patch clamping of isolated fiber cells confirms that spatially distinct Cl- influx and efflux pathways exist in the cortex of the rat lens. Investigative Ophthalmology & Visual Science, 50(8), 3808-3818. https://doi.org/10.1167/iovs.08-2680

PURPOSE. To test the hypothesis that lens fiber cells use different combinations of transport proteins to mediate Cl influx and efflux in order to regulate their steady state volume.

METHODS. Cells were isolated from rat lenses by enzymatic disso... Read More about Whole-cell patch clamping of isolated fiber cells confirms that spatially distinct Cl- influx and efflux pathways exist in the cortex of the rat lens.