Non-invasive isotope-based hydrodynamic imaging in plant roots at cellular resolution

Couvreur, Valentin; Pascut, Flavius C; Dietrich, Daniela; Leftley, Nicky; Reyt, Guilhem; Boursiac, Yann; Calvo-Polanco, Monica; Casimiro, Ilda; Maurel, Christophe; Salt, David E; Draye, Xavier; Wells, Darren M; Bennett, Malcolm J; Webb, Kevin F

Abstract

A key impediment to studying water-related mechanisms in plants is the inability to noninvasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling, can achieve this goal - monitoring deuterated water fluxes within living root tissues at cell- and sub-second-scale resolutions. Raman imaging of water-transporting xylem vessels in Arabidopsis thaliana mutant roots reveals faster xylem water transport in endodermal diffusion barrier mutants. Furthermore, transverse line scans across the root suggest water transported via the root xylem does not re-enter outer root tissues nor the surrounding soil when en-route to shoot tissues if endodermal diffusion barriers are intact, thereby separating ‘two water worlds’ inside roots.

Citation

Couvreur, V., Pascut, F. C., Dietrich, D., Leftley, N., Reyt, G., Boursiac, Y., Calvo-Polanco, M., Casimiro, I., Maurel, C., Salt, D. E., Draye, X., Wells, D. M., Bennett, M. J., & Webb, K. F. (2022, May). Non-invasive isotope-based hydrodynamic imaging in plant roots at cellular resolution. Presented at EGU General Assembly 2022, Vienna, Austria and online

• Presentation Conference Type: Conference Paper (published)
• Conference Name: EGU General Assembly 2022
• Start Date: May 23, 2022
• End Date: May 27, 2022
• Acceptance Date: Mar 28, 2022
• Online Publication Date: May 26, 2022
• Publication Date: May 26, 2022
• Deposit Date: Nov 18, 2024
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.5194/egusphere-egu22-9558
• Public URL: https://nottingham-repository.worktribe.com/output/37605196
• Publisher URL: https://meetingorganizer.copernicus.org/EGU22/EGU22-9558.html