Roots branch towards water by post-translational modification of transcription factor ARF7

Orosa Puente, Beatriz; Leftley, Nicky; Von Wangenheim, Daniel; Banda, Jason; Anjil, Srivastava; Hill, Kristine; Truskina, Jekaterina; Bhosale, Rahul; Morris, Emily; Srivastava, Moumita; Kumpers, Britta; Goh, Tatsuaki; Fukaki, Hidehiro; Vermeer, Joop E.M.; Vernoux, Teva; Dinneny, Jos� R.; French, Andrew P.; Bishopp, Anthony; Sadanandom, Ari; Bennett, Malcolm

Abstract

Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells on the side of the root in contact with water. This differential expression pattern is regulated by post-translational modification of ARF7 with the SUMO protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.

Citation

Orosa Puente, B., Leftley, N., Von Wangenheim, D., Banda, J., Anjil, S., Hill, K., Truskina, J., Bhosale, R., Morris, E., Srivastava, M., Kumpers, B., Goh, T., Fukaki, H., Vermeer, J. E., Vernoux, T., Dinneny, J. R., French, A. P., Bishopp, A., Sadanandom, A., & Bennett, M. (2018). Roots branch towards water by post-translational modification of transcription factor ARF7. Science, 362(6421), 1407-1410. https://doi.org/10.1126/science.aau3956

• Journal Article Type: Article
• Acceptance Date: Nov 6, 2018
• Online Publication Date: Dec 21, 2018
• Publication Date: Dec 21, 2018
• Deposit Date: Dec 4, 2018
• Publicly Available Date: Jun 22, 2019
• Journal: Science
• Print ISSN: 0036-8075
• Electronic ISSN: 1095-9203
• Publisher: American Association for the Advancement of Science
• Peer Reviewed: Peer Reviewed
• Volume: 362
• Issue: 6421
• Pages: 1407-1410
• DOI: https://doi.org/10.1126/science.aau3956
• Public URL: https://nottingham-repository.worktribe.com/output/1365597
• Publisher URL: http://science.sciencemag.org/content/362/6421/1407
• Additional Information: This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science on vol. 362, 21st December 2018, DOI: 10.1126/science.aau3956
• Contract Date: Dec 4, 2018