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Roots branch towards water by post-translational modification of transcription factor ARF7

Orosa Puente, Beatriz; Leftley, Nicky; Von Wangenheim, Daniel; Banda, Jason; Anjil, Srivastava; Hill, Kristine; Truskina, Jekaterina; Bhosale, Rahul; Morris, Emily; Srivastava, Moumita; Kumpers, Britta; Goh, Tatsuaki; Fukaki, Hidehiro; Vermeer, Joop E.M.; Vernoux, Teva; Dinneny, José R.; French, Andrew P.; Bishopp, Anthony; Sadanandom, Ari; Bennett, Malcolm

Authors

Beatriz Orosa Puente

Daniel Von Wangenheim

Jason Banda

Srivastava Anjil

Kristine Hill

Jekaterina Truskina

RAHUL BHOSALE RAHUL.BHOSALE@NOTTINGHAM.AC.UK
Beacon Nottingham Research Fellow

Emily Morris

Moumita Srivastava

Britta Kumpers

Tatsuaki Goh

Hidehiro Fukaki

Joop E.M. Vermeer

Teva Vernoux

José R. Dinneny

Ari Sadanandom



Abstract

Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells on the side of the root in contact with water. This differential expression pattern is regulated by post-translational modification of ARF7 with the SUMO protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.

Journal Article Type Article
Publication Date Dec 21, 2018
Journal Science
Print ISSN 0036-8075
Electronic ISSN 1095-9203
Publisher American Association for the Advancement of Science
Peer Reviewed Peer Reviewed
Volume 362
Issue 6421
Pages 1407-1410
APA6 Citation Orosa Puente, B., Leftley, N., Von Wangenheim, D., Banda, J., Anjil, S., Hill, K., …Bennett, M. (2018). Roots branch towards water by post-translational modification of transcription factor ARF7. Science, 362(6421), 1407-1410. https://doi.org/10.1126/science.aau3956
DOI https://doi.org/10.1126/science.aau3956
Publisher URL http://science.sciencemag.org/content/362/6421/1407
Additional Information This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science on vol. 362, 21st December 2018, DOI: 10.1126/science.aau3956

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