All Outputs (53)

Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell-derived cardiomyocytes (2016)
Journal Article
Rajamohan, D., Kalra, S., Hoang, M. D., George, V., Staniforth, A., Russell, H., …Denning, C. (2016). Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell-derived cardiomyocytes. Stem Cells and Development, 25(6), https://doi.org/10.1089/scd.2015.0253

Automated planar patch clamp systems are widely used in drug evaluation studies because of their ability to provide accurate, reliable, and reproducible data in a high-throughput manner. Typically, CHO and HEK tumorigenic cell lines overexpressing si... Read More about Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell-derived cardiomyocytes.

High throughput screening for discovery of materials that control stem cell fate (2016)
Journal Article
Patel, A. K., Tibbitt, M., Celiz, A. D., Davies, M. C., Langer, R., Denning, C., …Anderson, D. G. (2016). High throughput screening for discovery of materials that control stem cell fate. Current Opinion in Solid State and Materials Science, 20(4), 202-211. https://doi.org/10.1016/j.cossms.2016.02.002

Insights into the complex stem cell niche have identified the cell–material interface to be a potent regulator of stem cell fate via material properties such as chemistry, topography and stiffness. In light of this, materials scientists have the oppo... Read More about High throughput screening for discovery of materials that control stem cell fate.

Highly efficient delivery of functional cargoes by the synergistic effect of GAG binding motifs and cell-penetrating peptides (2016)
Journal Article
Dixon, J. E., Osman, G., Morris, G. E., Markides, H., Rotherham, M., Bayoussef, Z., …Shakesheff, K. M. (2016). Highly efficient delivery of functional cargoes by the synergistic effect of GAG binding motifs and cell-penetrating peptides. Proceedings of the National Academy of Sciences, 113(3), E291-E299. https://doi.org/10.1073/pnas.1518634113

Protein transduction domains (PTDs) are powerful nongenetic tools that allow intracellular delivery of conjugated cargoes to modify cell behavior. Their use in biomedicine has been hampered by inefficient delivery to nuclear and cytoplasmic targets.... Read More about Highly efficient delivery of functional cargoes by the synergistic effect of GAG binding motifs and cell-penetrating peptides.

Cardiomyocytes from human pluripotent stem cells: from laboratory curiosity to industrial biomedical platform (2015)
Journal Article
Denning, C., Borgdorff, V., Crutchley, J., Firth, K. S., George, V., Kalra, S., …Young, L. E. (2016). Cardiomyocytes from human pluripotent stem cells: from laboratory curiosity to industrial biomedical platform. BBA - Biochimica et Biophysica Acta, 1863(7), https://doi.org/10.1016/j.bbamcr.2015.10.014

Cardiomyocytes from human pluripotent stem cells (hPSCs-CMs) could revolutionise biomedicine. Global burden of heart failure will soon reach USD $90bn, while unexpected cardiotoxicity underlies 28% of drug withdrawals. Advances in hPSC isolation, Cas... Read More about Cardiomyocytes from human pluripotent stem cells: from laboratory curiosity to industrial biomedical platform.

A defined synthetic substrate for serum-free culture of human stem cell derived cardiomyocytes with improved functional maturity identified using combinatorial materials microarrays (2015)
Journal Article
Patel, A. K., Celiz, A. D., Rajamohan, D., Anderson, D. G., Langer, R., Davies, M. C., …Denning, C. (2015). A defined synthetic substrate for serum-free culture of human stem cell derived cardiomyocytes with improved functional maturity identified using combinatorial materials microarrays. Biomaterials, 61, https://doi.org/10.1016/j.biomaterials.2015.05.019

Cardiomyocytes from human stem cells have applications in regenerative medicine and can provide models for heart disease and toxicity screening. Soluble components of the culture system such as growth factors within serum and insoluble components suc... Read More about A defined synthetic substrate for serum-free culture of human stem cell derived cardiomyocytes with improved functional maturity identified using combinatorial materials microarrays.

Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation (2015)
Journal Article
Smith, J. G. W., Celiz, A. D., Smith, J. G., Patel, A. K., Hook, A. L., Rajamohan, D., …Alexander, M. R. (2015). Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation. Advanced Materials, 27(27), 4006-4012. https://doi.org/10.1002/adma.201501351

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial... Read More about Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation.

Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation. (2015)
Journal Article
Ghita, A., Pascut, F. C., Sottile, V., Denning, C., & Notingher, I. (2015). Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation. EPJ Techniques and Instrumentation, 2, Article 6. https://doi.org/10.1140/epjti/s40485-015-0016-8

Stem cell therapy is widely acknowledged as a key medical technology of the 21st century which may provide treatments for many currently incurable diseases. These cells have an enormous potential for cell replacement therapies to cure diseases such a... Read More about Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation..

Materials for stem cell factories of the future (2014)
Journal Article
Celiz, A. D., Smith, J. G., Langer, R., Anderson, D. G., Barrett, D. A., Winkler, D. A., …Alexander, M. R. (2014). Materials for stem cell factories of the future. Nature Materials, 13(6), 570-579. https://doi.org/10.1038/nmat3972

The materials community is now identifying polymeric substrates that could permit translation of human pluripotent stem cells (hPSCs) from lab-based research to industrial scale biomedicine. Well defined materials are required to allow cell banking a... Read More about Materials for stem cell factories of the future.

Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation (2014)
Journal Article
Dixon, J. E., Shah, D. A., Rogers, C., Hall, S., Weston, N., Parmenter, C. D., …Shakesheff, K. M. (2014). Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation. Proceedings of the National Academy of Sciences, 111(15), 5580-5585. https://doi.org/10.1073/pnas.1319685111

The ability of materials to define the architecture and microenvironment experienced by cells provides new opportunities to direct the fate of human pluripotent stem cells (HPSCs) [Robinton DA, Daley GQ (2012) Nature 481(7381):295-305]. However, the... Read More about Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation.

Exon skipping and gene transfer restore dystrophin expression in human induced pluripotent stem cells-cardiomyocytes harboring DMD mutations (2013)
Journal Article
Dick, E., Kalra, S., Anderson, D., George, V., Ritso, M., Laval, S. H., …Denning, C. (2013). Exon skipping and gene transfer restore dystrophin expression in human induced pluripotent stem cells-cardiomyocytes harboring DMD mutations. Stem Cells and Development, 22(20), https://doi.org/10.1089/scd.2013.0135

With an incidence of 1:3,500 to 5,000 in male children, Duchenne muscular dystrophy (DMD) is an X-linked disorder in which progressive muscle degeneration occurs and affected boys usually die in their twenties or thirties. Cardiac involvement occurs... Read More about Exon skipping and gene transfer restore dystrophin expression in human induced pluripotent stem cells-cardiomyocytes harboring DMD mutations.

Allele-specific RNA interference rescues the long-QT syndrome phenotype in human-induced pluripotency stem cell cardiomyocytes (2013)
Journal Article
Matsa, E., Dixon, J. E., Medway, C., Georgiou, O., Patel, M. J., Morgan, K., …Denning, C. (2014). Allele-specific RNA interference rescues the long-QT syndrome phenotype in human-induced pluripotency stem cell cardiomyocytes. European Heart Journal, 35(16), 1078-1087. https://doi.org/10.1093/eurheartj/eht067

Aims Long-QT syndromes (LQTS) are mostly autosomal-dominant congenital disorders associated with a 1:1000 mutation frequency, cardiac arrest, and sudden death. We sought to use cardiomyocytes derived from human-induced pluripotency stem cells (hiPSCs... Read More about Allele-specific RNA interference rescues the long-QT syndrome phenotype in human-induced pluripotency stem cell cardiomyocytes.

Current status of drug screening and disease modelling in human pluripotent stem cells (2013)
Journal Article
Rajamohan, D., Matsa, E., Kalra, S., Crutchley, J., Patel, A., George, V., & Denning, C. (2013). Current status of drug screening and disease modelling in human pluripotent stem cells. BioEssays, 35(3), https://doi.org/10.1002/bies.201200053

The emphasis in human pluripotent stem cell (hPSC) technologies has shifted from cell therapy to in vitro disease modelling and drug screening. This review examines why this shift has occurred, and how current technological limitations might be overc... Read More about Current status of drug screening and disease modelling in human pluripotent stem cells.

5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development (2012)
Journal Article
Almeida, R. D., Loose, M., Sottile, V., Matsa, E., Denning, C., Young, L., …Ruzov, A. (2012). 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development. Epigenetics, 7(4), https://doi.org/10.4161/epi.19375

5-hydroxymethyl-cytosine (5-hmc) is a cytosine modification that is relatively abundant in mammalian pre-implantation embryos and embryonic stem cells (Esc) derived from mammalian blastocysts. Recent observations imply that both 5-hmc and Tet1/2/... Read More about 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development.