Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell-derived cardiomyocytes

Rajamohan, Divya; Kalra, Spandan; Hoang, Minh Duc; George, Vinoj; Staniforth, Andrew; Russell, Hugh; Yang, Xuebin; Denning, Chris

Abstract

Automated planar patch clamp systems are widely used in drug evaluation studies because of their ability to provide accurate, reliable, and reproducible data in a high-throughput manner. Typically, CHO and HEK tumorigenic cell lines overexpressing single ion channels are used since they can be harvested as high-density, homogenous, single-cell suspensions. While human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) are physiologically more relevant, these cells are fragile, have complex culture requirements, are inherently heterogeneous, and are expensive to produce, which has restricted their use on automated patch clamp (APC) devices. Here, we used high efficiency differentiation protocols to produce cardiomyocytes from six different hPSC lines for analysis on the Patchliner (Nanion Technologies GmbH) APC platform. We developed a two-step cell preparation protocol that yielded cell catch rates and whole-cell breakthroughs of ∼80%, with ∼40% of these cells allowing electrical activity to be recorded. The protocol permitted formation of long-lasting (>15 min), high quality seals (>2 GΩ) in both voltage- and current-clamp modes. This enabled density of sodium, calcium, and potassium currents to be evaluated, along with dose–response curves to their respective channel inhibitors, tetrodotoxin, nifedipine, and E-4031. Thus, we show the feasibility of using the Patchliner platform for automated evaluation of the electrophysiology and pharmacology of hPSC-CMs, which will enable considerable increase in throughput for reliable and efficient drug evaluation.

Citation

Rajamohan, D., Kalra, S., Hoang, M. D., George, V., Staniforth, A., Russell, H., …Denning, C. (2016). Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell-derived cardiomyocytes. Stem Cells and Development, 25(6), https://doi.org/10.1089/scd.2015.0253

• Journal Article Type: Article
• Acceptance Date: Jan 18, 2016
• Online Publication Date: Feb 23, 2016
• Publication Date: Mar 15, 2016
• Deposit Date: Oct 25, 2016
• Publicly Available Date: Oct 25, 2016
• Journal: Stem Cells and Development
• Print ISSN: 1547-3287
• Electronic ISSN: 1557-8534
• Publisher: Mary Ann Liebert
• Peer Reviewed: Peer Reviewed
• Volume: 25
• Issue: 6
• DOI: https://doi.org/10.1089/scd.2015.0253
• Public URL: https://nottingham-repository.worktribe.com/output/780429
• Publisher URL: http://online.liebertpub.com/doi/10.1089/scd.2015.0253
• Contract Date: Oct 25, 2016