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5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development

Almeida, Rimple D.; Loose, Matthew; Sottile, Virginie; Matsa, Elena; Denning, Chris; Young, Lorraine; Johnson, Andrew D.; Gering, Martin; Ruzov, Alexey

Authors

Rimple D. Almeida

Matthew Loose

Virginie Sottile

Elena Matsa

Chris Denning

Lorraine Young

Andrew D. Johnson

Martin Gering

Alexey Ruzov Alexey.Ruzov@nottingham.ac.uk



Abstract

5-hydroxymethyl-cytosine (5-hmc) is a cytosine modification that is relatively abundant in mammalian pre-implantation
embryos and embryonic stem cells (Esc) derived from mammalian blastocysts. Recent observations imply that both
5-hmc and Tet1/2/3 proteins, catalyzing the conversion of 5-methyl-cytosine to 5-hmc, may play an important role in
self renewal and differentiation of Escs. here we assessed the distribution of 5-hmc in zebrafish and chick embryos
and found that, unlike in mammals, 5-hmc is immunochemically undetectable in these systems before the onset of
organogenesis. In addition, Tet1/2/3 transcripts are either low or undetectable at corresponding stages of zebrafish
development. however, 5-hmc is enriched in later zebrafish and chick embryos and exhibits tissue-specific distribution in adult zebrafish. Our findings show that 5-hmc enrichment of non-committed cells is not a universal feature of vertebrate development and give insights both into evolution of embryonic pluripotency and the potential role of 5-hmc in its regulation.

Journal Article Type Article
Publication Date Apr 1, 2012
Journal Epigenetics
Print ISSN 1559-2294
Electronic ISSN 1559-2294
Publisher Taylor & Francis
Peer Reviewed Peer Reviewed
Volume 7
Issue 4
APA6 Citation Almeida, R. D., Loose, M., Sottile, V., Matsa, E., Denning, C., Young, L., …Ruzov, A. (2012). 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development. Epigenetics, 7(4), doi:10.4161/epi.19375
DOI https://doi.org/10.4161/epi.19375
Publisher URL https://www.landesbioscience.com/journals/epigenetics/article/19375/
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0





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