5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development

Almeida, Rimple D.; Loose, Matthew; Sottile, Virginie; Matsa, Elena; Denning, Chris; Young, Lorraine; Johnson, Andrew D.; Gering, Martin; Ruzov, Alexey

Abstract

5-hydroxymethyl-cytosine (5-hmc) is a cytosine modification that is relatively abundant in mammalian pre-implantation
embryos and embryonic stem cells (Esc) derived from mammalian blastocysts. Recent observations imply that both
5-hmc and Tet1/2/3 proteins, catalyzing the conversion of 5-methyl-cytosine to 5-hmc, may play an important role in
self renewal and differentiation of Escs. here we assessed the distribution of 5-hmc in zebrafish and chick embryos
and found that, unlike in mammals, 5-hmc is immunochemically undetectable in these systems before the onset of
organogenesis. In addition, Tet1/2/3 transcripts are either low or undetectable at corresponding stages of zebrafish
development. however, 5-hmc is enriched in later zebrafish and chick embryos and exhibits tissue-specific distribution in adult zebrafish. Our findings show that 5-hmc enrichment of non-committed cells is not a universal feature of vertebrate development and give insights both into evolution of embryonic pluripotency and the potential role of 5-hmc in its regulation.

Citation

Almeida, R. D., Loose, M., Sottile, V., Matsa, E., Denning, C., Young, L., …Ruzov, A. (2012). 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development. Epigenetics, 7(4), https://doi.org/10.4161/epi.19375

• Journal Article Type: Article
• Publication Date: Apr 1, 2012
• Deposit Date: Mar 27, 2014
• Publicly Available Date: Mar 27, 2014
• Journal: Epigenetics
• Print ISSN: 1559-2294
• Electronic ISSN: 1559-2294
• Publisher: Taylor & Francis Open
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 4
• DOI: https://doi.org/10.4161/epi.19375
• Public URL: https://nottingham-repository.worktribe.com/output/1007583
• Publisher URL: https://www.landesbioscience.com/journals/epigenetics/article/19375/