Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation

Smith, James G. W.; Celiz, Adam D.; Smith, James G.W.; Patel, Asha K.; Hook, Andrew L.; Rajamohan, Divya; George, Vinoj T.; Flatt, Luke; Patel, Minal J.; Epa, Vidana C.; Singh, Taranjit; Langer, Robert; Anderson, Daniel G.; Allen, Nicholas D.; Hay, David C.; Winkler, David A.; Barrett, David A.; Davies, Martyn C.; Young, Lorraine E.; Denning, Chris; Alexander, Morgan R.

Abstract

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.

Citation

Smith, J. G. W., Celiz, A. D., Smith, J. G., Patel, A. K., Hook, A. L., Rajamohan, D., George, V. T., Flatt, L., Patel, M. J., Epa, V. C., Singh, T., Langer, R., Anderson, D. G., Allen, N. D., Hay, D. C., Winkler, D. A., Barrett, D. A., Davies, M. C., Young, L. E., Denning, C., & Alexander, M. R. (2015). Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation. Advanced Materials, 27(27), 4006-4012. https://doi.org/10.1002/adma.201501351

• Journal Article Type: Article
• Acceptance Date: Apr 23, 2015
• Online Publication Date: Jun 1, 2015
• Publication Date: Jul 15, 2015
• Deposit Date: Dec 7, 2017
• Publicly Available Date: Dec 7, 2017
• Journal: Advanced Materials
• Print ISSN: 0935-9648
• Electronic ISSN: 1521-4095
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 27
• Issue: 27
• Pages: 4006-4012
• DOI: https://doi.org/10.1002/adma.201501351
• Keywords: high throughput; human pluripotent stem cells; materials discovery; polymer microarrays; stem cell differentiation
• Public URL: https://nottingham-repository.worktribe.com/output/756850
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/adma.201501351/full
• Contract Date: Dec 7, 2017