PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays

Stewart, Chloe L.; Hook, Andrew L.; Zelzer, Mischa; Marlow, Maria; Piccinini, Anna M.

Abstract

We identified that PLGA-PEG-PLGA hydrogels, which have been used in human clinical trials and possess a demonstrable safety profile, induced significant cytotoxicity in conventional in vitro assays. This major contradiction may lead to inconsistent and misleading toxicology due to the limited biological representation of these assays. Cytotoxicity evaluation is a crucial element of screening the biological response to new biomaterials. However, as standard test methods do not recapitulate the in vivo environment, tailored adaptations may be required to reflect the true biological response elicited toward novel biomaterials.

Citation

Stewart, C. L., Hook, A. L., Zelzer, M., Marlow, M., & Piccinini, A. M. (2024). PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays. Cell Biochemistry and Function, 42(5), Article e4097. https://doi.org/10.1002/cbf.4097

• Journal Article Type: Other
• Acceptance Date: Jul 10, 2024
• Online Publication Date: Jul 15, 2024
• Publication Date: 2024-07
• Deposit Date: Jul 16, 2024
• Publicly Available Date: Jul 16, 2024
• Journal: Cell Biochemistry and Function
• Print ISSN: 0263-6484
• Electronic ISSN: 1099-0844
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 42
• Issue: 5
• Article Number: e4097
• DOI: https://doi.org/10.1002/cbf.4097
• Keywords: biomaterial; cytotoxicity; fibroblast; hydrogel; monocyte; viability
• Public URL: https://nottingham-repository.worktribe.com/output/37307112
• Publisher URL: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/cbf.4097
• Additional Information: Received: 2024-03-13; Accepted: 2024-07-10; Published: 2024-07-15