Heba Khateb
Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS
Khateb, Heba; Hook, Andrew L.; Kern, Stefanie; Watts, Julie A.; Singh, Sonali; Jackson, Darryl; Marinez-Pomares, Luisa; Williams, Paul; Alexander, Morgan R.
Authors
ANDREW HOOK ANDREW.HOOK@NOTTINGHAM.AC.UK
Assistant Professor
Stefanie Kern
Julie A. Watts
SONALI SINGH SONALI.SINGH@NOTTINGHAM.AC.UK
Research Development Manager
Darryl Jackson
LUISA MARTINEZ-POMARES LUISA.M@NOTTINGHAM.AC.UK
Professor of Innate Immunity and Inflammation
PAUL WILLIAMS PAUL.WILLIAMS@NOTTINGHAM.AC.UK
Professor of Molecular Microbiology
MORGAN ALEXANDER MORGAN.ALEXANDER@NOTTINGHAM.AC.UK
Professor of Biomedical Surfaces
Abstract
Secondary ion mass spectrometry (SIMS) offers advantages over both liquid extraction mass spectrometry and matrix assisted laser desorption mass spectrometry in that it provides the direct in situ analysis of molecules and has the potential to preserve the 3D location of an analyte in a sample. Polysaccharides are recognized as challenging analytes in the mass spectrometry of liquids and are also difficult to identify and assign using SIMS. Psl is an exopolysaccharide produced by Pseudomonas aeruginosa, which plays a key role in biofilm formation and maturation. In this Letter, we describe the use of the OrbiTrap analyzer with SIMS (3D OrbiSIMS) for the label-free mass spectrometry of Psl, taking advantage of its high mass resolving power for accurate secondary ion assignment. We study a P. aeruginosa biofilm and compare it with purified Psl to enable the assignment of secondary ions specific to the Psl structure. This resulted in the identification of 17 peaks that could confidently be ascribed to Psl fragments within the biofilm matrix. The complementary approach of the following neutral loss sequences is also shown to identify multiple oligosaccharide fragments without the requirement of a biological reference sample.
Citation
Khateb, H., Hook, A. L., Kern, S., Watts, J. A., Singh, S., Jackson, D., …Alexander, M. R. (2023). Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS. Biointerphases, 18(3), Article 031007. https://doi.org/10.1116/6.0002604
Journal Article Type | Article |
---|---|
Acceptance Date | May 9, 2023 |
Online Publication Date | May 31, 2023 |
Publication Date | 2023-05 |
Deposit Date | Jun 7, 2023 |
Publicly Available Date | Jun 7, 2023 |
Journal | Biointerphases |
Print ISSN | 1934-8630 |
Electronic ISSN | 1559-4106 |
Publisher | American Vacuum Society |
Peer Reviewed | Peer Reviewed |
Volume | 18 |
Issue | 3 |
Article Number | 031007 |
DOI | https://doi.org/10.1116/6.0002604 |
Keywords | General Physics and Astronomy; General Biochemistry, Genetics and Molecular Biology; General Materials Science; Biomaterials; General Chemistry |
Public URL | https://nottingham-repository.worktribe.com/output/21378174 |
Publisher URL | https://pubs.aip.org/avs/bip/article/18/3/031007/2893559/Identification-of-Pseudomonas-aeruginosa |
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Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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