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All Outputs (17)

Disruption of Epithelial Barriers for Molecular Delivery Enhancement or Extraction of Extracellular Fluids (2024)
Patent
Webb, K., Moradi, E., & Foss, A. (2024). Disruption of Epithelial Barriers for Molecular Delivery Enhancement or Extraction of Extracellular Fluids. WO2024009101A1. UK

The invention provides a method of delivering a payload molecule across an epithelial tissue barrier, the method comprising: applying the payload molecule to the epithelial tissue barrier, and additionally applying an agent to the epithelial tissue b... Read More about Disruption of Epithelial Barriers for Molecular Delivery Enhancement or Extraction of Extracellular Fluids.

Investigation of Exhaust Particles on Different TEM Grids: A Comparison between Graphene Oxide and Silicon Nitride Grids (2023)
Journal Article
Lagana, S., Akifjevs, R., Rocca, A. L., Cairns, A., Fay, M. W., & Webb, K. F. (2023). Investigation of Exhaust Particles on Different TEM Grids: A Comparison between Graphene Oxide and Silicon Nitride Grids. SAE Technical Papers, 2023-32-0123, https://doi.org/10.4271/2023-32-0123

Two different TEM (Transmission Electron Microscopy) grids - graphene oxide (GO) and silicon nitride (SiN) - were used to capture the particulates emitted with the exhaust of a modern 1.0 L GDI (Gasoline Direct Injection) engine. One speed-load condi... Read More about Investigation of Exhaust Particles on Different TEM Grids: A Comparison between Graphene Oxide and Silicon Nitride Grids.

The Modular μSiM: a Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro (2022)
Journal Article
McCloskey, M. C., Kasap, P., Ahmad, S. D., Su, S., Chen, K., Mansouri, M., …McGrath, J. L. (2022). The Modular μSiM: a Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro. Advanced Healthcare Materials, 11(18), Article 2200804. https://doi.org/10.1002/adhm.202200804

Advanced in vitro tissue chip models can reduce and replace animal experimentation and may eventually support "on-chip" clinical trials. To realize this potential, however, tissue chip platforms must be both mass-produced and reconfigurable to allow... Read More about The Modular μSiM: a Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro.

Non-invasive hydrodynamic imaging in plant roots at cellular resolution (2021)
Journal Article
Pascut, F. C., Couvreur, V., Dietrich, D., Leftley, N., Reyt, G., Boursiac, Y., …Webb, K. F. (2021). Non-invasive hydrodynamic imaging in plant roots at cellular resolution. Nature Communications, 12(1), 1-7. https://doi.org/10.1038/s41467-021-24913-z

A key impediment to studying water-related mechanisms in plants is the inability to non-invasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelli... Read More about Non-invasive hydrodynamic imaging in plant roots at cellular resolution.

Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells (2020)
Journal Article
Pérez-Cota, F., Fuentes-Domínguez, R., La Cavera III, S., Hardiman, W., Yao, M., Setchfield, K., …Clark, M. (2020). Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells. Journal of Applied Physics, 128(16), Article 160902. https://doi.org/10.1063/5.0023744

© 2020 Author(s). Characterization of the elasticity of biological cells is growing as a new way to gain insight into cell biology. Cell mechanics are related to most aspects of cellular behavior, and applications in research and medicine are broad.... Read More about Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells.

Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro (2020)
Journal Article
Harun, M., Marsh, V., Elsaied, N. A., Webb, K. F., & Elsheikha, H. M. (2020). Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro. Brain Research, 1746, Article 147002. https://doi.org/10.1016/j.brainres.2020.147002

Toxoplasma gondii can cause parasitic encephalitis, a life-threatening infection that predominately occurs in immunocompromised individuals. T. gondii has the ability to invade the brain, but the mechanisms by which this parasite crosses the blood–br... Read More about Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro.

Surface plasmon resonance imaging of excitable cells (2019)
Journal Article
Howe, C. L., Webb, K. F., Abayzeed, S. A., Anderson, D. J., Denning, C., & Russell, N. A. (2019). Surface plasmon resonance imaging of excitable cells. Journal of Physics D: Applied Physics, 52(10), Article 104001. https://doi.org/10.1088/1361-6463/aaf849

Surface plasmons (SPs) are surface charge density oscillations occuring at a metal/dieletric interface and are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and... Read More about Surface plasmon resonance imaging of excitable cells.

Surface Plasmon Resonance Imaging of Excitable Cells (2018)
Working Paper
Howe, C. L., Webb, K. F., Abayzeed, S. A., Anderson, D. J., Denning, C., & Russell, N. A. Surface Plasmon Resonance Imaging of Excitable Cells

Surface plasmons are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and biological assays. In these systems, a surface plasmon resonance (SPR)-based sensor detect... Read More about Surface Plasmon Resonance Imaging of Excitable Cells.

Non destructive evaluation of biological cells (2018)
Journal Article
Pérez-Cota, F., Smith, R. J., Moradi, E., Webb, K. F., & Clark, M. (2019). Non destructive evaluation of biological cells. AIP Conference Proceedings, 2102, https://doi.org/10.1063/1.5099737

© 2019 Author(s). Regenerative medicine promises to be the next revolution in health care. This technology, which will be the first systematic manufacturing of biological parts for human consumption, requires non destructive evaluatioin (NDE) thechni... Read More about Non destructive evaluation of biological cells.

Sensitive detection of voltage transients using differential intensity surface plasmon resonance system (2017)
Journal Article
Abayzeed, S. A., Smith, R. J., Webb, K. F., Somekh, M. G., & See, C. W. (in press). Sensitive detection of voltage transients using differential intensity surface plasmon resonance system. Optics Express, 25(25), https://doi.org/10.1364/OE.25.031552

This paper describes theoretical and experimental study of the fundamentals of using surface plasmon resonance (SPR) for label-free detection of voltage. Plasmonic voltage sensing relies on the capacitive properties of metal-electrolyte interface tha... Read More about Sensitive detection of voltage transients using differential intensity surface plasmon resonance system.

Responsivity of the differential-intensity surface plasmon resonance instrument (2016)
Journal Article
Abayzeed, S. A., Smith, R. J., Webb, K. F., Somekh, M. G., & See, C. W. (2016). Responsivity of the differential-intensity surface plasmon resonance instrument. Sensors and Actuators B: Chemical, 235, 627-635. https://doi.org/10.1016/j.snb.2016.05.117

Surface plasmon resonance is used for the sensitive measurement of minute concentrations of bio-analytes and probing of electrochemical processes. Typical refractive index sensitivity, for the intensity approach, is around 10−6 refractive index units... Read More about Responsivity of the differential-intensity surface plasmon resonance instrument.

Cell imaging using sub-optical wavelength ultrasonics (2015)
Conference Proceeding
Clark, M., Marques, L., Moradi, E., Perez-Cota, F., Smith, R. J., & Webb, K. F. (2015). Cell imaging using sub-optical wavelength ultrasonics. In Proceedings Asia Communications and Photonics Conference 2015. https://doi.org/10.1364/acpc.2015.asu5h.1

At GHz frequencies the wavelength of ultrasound becomes comparable or smaller than the wavelength of light and so offers an intriguing way to image at the small scale. In this paper we report on new developments in pico-second laser ultrasonics that... Read More about Cell imaging using sub-optical wavelength ultrasonics.

Sub-100 nm resolution microscopy based on proximity projection grating scheme (2015)
Journal Article
Hu, F., Somekh, M. G., Albutt, D. J., Webb, K., Moradi, E., & See, C. W. (2015). Sub-100 nm resolution microscopy based on proximity projection grating scheme. Scientific Reports, 5, https://doi.org/10.1038/srep08589

Structured illumination microscopy (SIM) has been widely used in life science imaging applications. The maximum resolution improvement of SIM, compared to conventional bright field system is a factor of 2. Here we present an approach to structured il... Read More about Sub-100 nm resolution microscopy based on proximity projection grating scheme.

Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage (2012)
Journal Article
Gunning, S. J., Chung, K. K., Donaldson, P. J., & Webb, K. F. (2012). Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage. American Journal of Physiology - Cell Physiology, 303(12), C1252-C1259. https://doi.org/10.1152/ajpcell.00237.2012

The initiation of lens cataract has long been associated with the development of a membrane "leak" in lens fiber cells that depolarizes the lens intracellular potential and elevates intracellular Na+ and Ca2+ concentrations. It has been proposed that... Read More about Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage.

Regulation of lens volume: Implications for lens transparency (2009)
Journal Article
Donaldson, P. J., Chee, K. S. N., Lim, J. C., & Webb, K. F. (2009). Regulation of lens volume: Implications for lens transparency. Experimental Eye Research, 88(2), 144-150. https://doi.org/10.1016/j.exer.2008.05.011

Lens transparency is critically dependent on the maintenance of an ordered tissue architecture, and disruption of this order leads to light scatter and eventually lens cataract. Hence the volume of the fiber cells that make up the bulk of the lens ne... Read More about Regulation of lens volume: Implications for lens transparency.

Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens (2008)
Journal Article
Hu, R. G., Suzuki-Kerr, H., Webb, K. F., Rhodes, J. D., Collison, D. J., Duncan, G., & Donaldson, P. J. (2008). Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens. Experimental Eye Research, 87(2), 137-146. https://doi.org/10.1016/j.exer.2008.05.008

Extracellular ATP has been shown to mobilize intracellular Ca2+ in cultured ovine lens epithelial cells and in human lens epithelium, suggesting a role for purines in the modulation of lens transparency. In this study, we characterized the expression... Read More about Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens.