All Outputs (108)

Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium (2015)
Journal Article
Freschi, L., Jeukens, J., Kukavica-Ibrulj, I., Boyle, B., Dupont, M.-J., Laroche, J., Larose, S., Maaroufi, H., Fothergill, J. L., Moore, M., Winsor, G. L., Aaron, S. D., Barbeau, J., Bell, S. C., Burns, J. L., Cámara, M., Cantin, A., Charette, S. J., Dewar, K., Déziel, É., …Levesque, R. C. (in press). Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium. Frontiers in Microbiology, 6(1036), https://doi.org/10.3389/fmicb.2015.01036

The International Pseudomonas aeruginosa Consortium is sequencing over 1000 genomes and building an analysis pipeline for the study of Pseudomonas genome evolution, antibiotic resistance and virulence genes. Metadata, including genomic and phenotypic... Read More about Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium.

Conflict of interest and signal interference lead to the breakdown of honest signalling (2015)
Journal Article
Popat, R., Pollitt, E. J., Harrison, F., Naghra, H., Hong, K. W., Chan, K. G., Griffin, A., Williams, P., Brown, S. P., West, S. A., & Diggle, S. P. (2015). Conflict of interest and signal interference lead to the breakdown of honest signalling. Evolution, 69(9), https://doi.org/10.1111/evo.12751

Animals use signals to coordinate a wide range of behaviours, from feeding offspring to predator avoidance. This poses an evolutionary problem, because individuals could potentially signal dishonestly to coerce others into behaving in ways that benef... Read More about Conflict of interest and signal interference lead to the breakdown of honest signalling.

Biotic inactivation of the Pseudomonas aeruginosa quinolone signal molecule (2015)
Journal Article
Soh, E. Y.-C., Chhabra, S. R., Halliday, N., Heeb, S., Müller, C., Birmes, F. S., Fetzner, S., Cámara, M., Chan, K. G., & Williams, P. (2015). Biotic inactivation of the Pseudomonas aeruginosa quinolone signal molecule. Environmental Microbiology, 17(11), https://doi.org/10.1111/1462-2920.12857

In Pseudomonas aeruginosa, quorum sensing (QS) regulates the production of secondary metabolites, many of which are antimicrobials that impact on polymicrobial community composition. Consequently, quenching QS modulates the environmental impact of P.... Read More about Biotic inactivation of the Pseudomonas aeruginosa quinolone signal molecule.

Granulocyte-macrophage colony stimulatory factor enhances the pro-inflammatory response of interferon-γ-treated macrophages to pseudomonas aeruginosa infection (2015)
Journal Article
Singh, S., Barr, H. L., Liu, Y.-C., Robins, A., Heeb, S., Williams, P., Fogarty, A. W., Cámara, M., & Martinez-Pomares, L. (2015). Granulocyte-macrophage colony stimulatory factor enhances the pro-inflammatory response of interferon-γ-treated macrophages to pseudomonas aeruginosa infection. PLoS ONE, 10(2), Article e0117447. https://doi.org/10.1371/journal.pone.0117447

Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe infections at compromised epithelial surfaces, such those found in burns, wounds, and in lungs damaged by mechanical ventilation or recurrent infections, particularly in cystic... Read More about Granulocyte-macrophage colony stimulatory factor enhances the pro-inflammatory response of interferon-γ-treated macrophages to pseudomonas aeruginosa infection.

Pseudomonas aeruginosa quorum sensing molecules correlate with clinical status in cystic fibrosis (2015)
Journal Article
Barr, H. L., Halliday, N., Cámara, M., Barrett, D. A., Williams, P., Forrester, D. L., Simms, R., Smyth, A. R., Honeybourne, D., Whitehouse, J. L., Nash, E. F., Dewar, J., Clayton, A., Knox, A. J., & Fogarty, A. W. (2015). Pseudomonas aeruginosa quorum sensing molecules correlate with clinical status in cystic fibrosis. European Respiratory Journal, 46(4), 1046-1054. https://doi.org/10.1183/09031936.00225214

ABSTRACT Pseudomonas aeruginosa produces quorum sensing signal molecules that are potential biomarkers for infection.
A prospective study of 60 cystic fibrosis patients with chronic P. aeruginosa, who required intravenous antibiotics for pulmonary e... Read More about Pseudomonas aeruginosa quorum sensing molecules correlate with clinical status in cystic fibrosis.

[18F]FDG-6-P as a novel in vivo tool for imaging staphylococcal infections (2015)
Journal Article
Mills, B., Awais, R., Luckett, J., Turton, D., Williams, P., Perkins, A., & Hill, P. (2015). [18F]FDG-6-P as a novel in vivo tool for imaging staphylococcal infections. EJNMMI Research, 5, Article 13. https://doi.org/10.1186/s13550-015-0095-1

Background
Management of infection is a major clinical problem. Staphylococcus aureus is a Gram-positive bacterium which colonises approximately one third of the adult human population. Staphylococcal infections can be life-threatening and are frequ... Read More about [18F]FDG-6-P as a novel in vivo tool for imaging staphylococcal infections.

Genome-wide evaluation of the interplay between Caenorhabditis elegans and Yersinia pseudotuberculosis during in vivo biofilm formation. (2014)
Journal Article
Joshua, G. W., Atkinson, S., Goldstone, R. J., Patrick, H. L., Stabler, R. A., Purves, J., Cámara, M., Williams, P., & Wren, B. W. (2015). Genome-wide evaluation of the interplay between Caenorhabditis elegans and Yersinia pseudotuberculosis during in vivo biofilm formation. Infection and Immunity, 83(1), Article 17. https://doi.org/10.1128/IAI.00110-14

The formation of an incapacitating biofilm on Caenorhabditis elegans by Yersinia pseudotuberculosis represents a tractable model for investigating the genetic basis for host-pathogen interplay during the biofilm-mediated infection of a living surface... Read More about Genome-wide evaluation of the interplay between Caenorhabditis elegans and Yersinia pseudotuberculosis during in vivo biofilm formation..

Bacterial Attachment to Polymeric Materials Correlates with Molecular Flexibility and Hydrophilicity (2014)
Journal Article
Sanni, O., Chang, C.-Y., Anderson, D. G., Langer, R., Davies, M. C., Williams, P. M., Williams, P., Alexander, M. R., & Hook, A. L. (2015). Bacterial Attachment to Polymeric Materials Correlates with Molecular Flexibility and Hydrophilicity. Advanced Healthcare Materials, 4(5), 695-701. https://doi.org/10.1002/adhm.201400648

A new class of material resistant to bacterial attachment has been discovered that is formed from polyacrylates with hydrocarbon pendant groups. In this study, the relationship between the nature of the hydrocarbon moiety and resistance to bacteria i... Read More about Bacterial Attachment to Polymeric Materials Correlates with Molecular Flexibility and Hydrophilicity.

Predicting the virulence of MRSA from its genome sequence (2014)
Journal Article
Laabei, M., Recker, M., Rudkin, J. K., Aldeljawi, M., Gulay, Z., Sloan, T. J., Williams, P., Endres, J. L., Bayles, K. W., Fey, P. D., Kumar Yajjala, V., Widhelm, T., Hawkins, E., Lewis, K., Parfett, S., Scowen, L., Peacock, S. J., Holden, M., Wilson, D., Read, T. D., …Massey, R. C. (2014). Predicting the virulence of MRSA from its genome sequence. Genome Research, 24(5), 839-849. https://doi.org/10.1101/gr.165415.113

LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules (2014)
Book Chapter
Ortori, C. A., Ortori, C., Halliday, N., Cámara, M., Williams, P., & Barrett, D. A. (2014). LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules. In A. Filloux, & J.-L. Ramos (Eds.), Pseudomonas Methods and Protocols (255-270). Springer. https://doi.org/10.1007/978-1-4939-0473-0_21

© Springer Science+Business Media New York 2014. Extracts taken from spent growth media from Pseudomonas aeruginosa can be analyzed for N -acyl- L - homoserine lactones and 2-alkyl-4-(1 H)-quinolones (AQs), including the known quorum sensing signalli... Read More about LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules.

Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors (2014)
Journal Article
Murray, E. J., Crowley, R. C., Truman, A., Clarke, S. R., Cottam, J. A., Jadhav, G. P., Steele, V. R., O’Shea, P., Lindholm, C., Cockayne, A., Chhabra, S. R., Chan, W. C., & Williams, P. (2014). Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors. Journal of Medicinal Chemistry, 57(6), 2813-2819. https://doi.org/10.1021/jm500215s

A series of 3-oxo-C 12 -HSL, tetramic acid, and tetronic acid analogues were synthesized to gain insights into the structural requirements for quorum sensing inhibition in Staphylococcus aureus. Compounds active against agr were noncompetitive inhibi... Read More about Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors.

Thermally Switchable Polymers Achieve Controlled Escherichia coli Detachment (2014)
Journal Article
Hook, A. L., Chang, C., Scurr, D. J., Langer, R., Anderson, D. G., Williams, P., Davies, M. C., & Alexander, M. R. (2014). Thermally Switchable Polymers Achieve Controlled Escherichia coli Detachment. Advanced Healthcare Materials, 3(7), 1020-1025. https://doi.org/10.1002/adhm.201300518

The thermally triggered release of up to 96% of attached uropathogenic E. coli is achieved on two polymers with opposite changes in surface wettability upon reduction in temperature. This demonstrates that the bacterial attachment to a surface cannot... Read More about Thermally Switchable Polymers Achieve Controlled Escherichia coli Detachment.

Modelling and Prediction of Bacterial Attachment to Polymers (2013)
Journal Article
Epa, V., Hook, A. L., Chang, C., Yang, J., Langer, R., Anderson, D. G., Williams, P., Davies, M. C., Alexander, M. R., & Winkler, D. A. (2014). Modelling and Prediction of Bacterial Attachment to Polymers. Advanced Functional Materials, 24(14), 2085-2093. https://doi.org/10.1002/adfm.201302877

Infection by pathogenic bacteria on implanted and indwelling medical devices during surgery causes large morbidity and mortality worldwide. Attempts to ameliorate this important medical issue have included development of antimicrobial surfaces on mat... Read More about Modelling and Prediction of Bacterial Attachment to Polymers.

Structural basis for native agonist and synthetic inhibitor recognition by the Pseudomonas aeruginosa quorum sensing regulator PqsR (MvfR) (2013)
Journal Article
Ilangovan, A., Fletcher, M., Rampioni, G., Pustelny, C., Rumbaugh, K., Heeb, S., Cámara, M., Truman, A., Chhabra, S. R., Emsley, J., & Williams, P. (2013). Structural basis for native agonist and synthetic inhibitor recognition by the Pseudomonas aeruginosa quorum sensing regulator PqsR (MvfR). PLoS Pathogens, 9(7), Article e1003508. https://doi.org/10.1371/journal.ppat.1003508

Bacterial populations co-ordinate gene expression collectively through quorum sensing (QS), a cell-to-cell communication mechanism employing diffusible signal molecules. The LysR-type transcriptional regulator (LTTR) protein PqsR (MvfR) is a key comp... Read More about Structural basis for native agonist and synthetic inhibitor recognition by the Pseudomonas aeruginosa quorum sensing regulator PqsR (MvfR).

Interference with the germination and growth of Ulva zoospores by quorum-sensing molecules from Ulva-associated epiphytic bacteria (2013)
Journal Article
Twigg, M. S., Tait, K., Williams, P., Atkinson, S., & Cámara, M. (2014). Interference with the germination and growth of Ulva zoospores by quorum-sensing molecules from Ulva-associated epiphytic bacteria. Environmental Microbiology, 16(2), 445-453. https://doi.org/10.1111/1462-2920.12203

Ulva zoospores preferentially settle on N‐acylhomoserine lactone (AHL) producing marine bacterial biofilms. To investigate whether AHL signal molecules also affect the success and rate of zoospore germination in addition to zoospore attraction, the e... Read More about Interference with the germination and growth of Ulva zoospores by quorum-sensing molecules from Ulva-associated epiphytic bacteria.

Discovery of novel materials with broad resistance to bacterial attachment using combinatorial polymer microarrays (2013)
Journal Article
Hook, A. L., Chang, C.-Y., Yang, J., Atkinson, S., Langer, R., Anderson, D. G., Davies, M. C., Williams, P., & Alexander, M. R. (2013). Discovery of novel materials with broad resistance to bacterial attachment using combinatorial polymer microarrays. Advanced Materials, 25(18), https://doi.org/10.1002/adma.201204936

A new class of bacteria-attachment-resistant materials is discovered using a multi-generation polymer microarray methodology that reduces bacterial attachment by up to 99.3% compared with a leading commercially available silver hydrogel anti-bacteria... Read More about Discovery of novel materials with broad resistance to bacterial attachment using combinatorial polymer microarrays.

Attenuating Staphylococcus aureus virulence gene regulation: A medicinal chemistry perspective (2013)
Journal Article
Gordon, C. P., Williams, P., & Chan, W. C. (2013). Attenuating Staphylococcus aureus virulence gene regulation: A medicinal chemistry perspective. Journal of Medicinal Chemistry, 56(4), 1389-1404. https://doi.org/10.1021/jm3014635

Virulence gene expression in Staphylococcus aureus is tightly regulated by intricate networks of transcriptional regulators and two-component signal transduction systems. There is now an emerging body of evidence to suggest that the blockade of S. au... Read More about Attenuating Staphylococcus aureus virulence gene regulation: A medicinal chemistry perspective.

High throughput discovery of thermo-responsive materials using water contact angle measurements and time-of-flight secondary ion mass spectrometry (2013)
Journal Article
Hook, A. L., Scurr, D. J., Anderson, D. G., Langer, R., Williams, P., Davies, M. C., & Alexander, M. R. (2013). High throughput discovery of thermo-responsive materials using water contact angle measurements and time-of-flight secondary ion mass spectrometry. Surface and Interface Analysis, 45(1), https://doi.org/10.1002/sia.4910

Switchable materials that alter their chemical or physical properties in response to external stimuli allow for temporal control of material-biological interactions, thus, are of interest for many biomaterial applications. Our interest is the discove... Read More about High throughput discovery of thermo-responsive materials using water contact angle measurements and time-of-flight secondary ion mass spectrometry.

Combinatorial discovery of polymers resistant to bacterial attachment (2012)
Journal Article
Hook, A. L., Chang, C.-Y., Yang, J., Luckett, J., Cockayne, A., Atkinson, S., Mei, Y., Bayston, R., Irvine, D. J., Langer, R., Anderson, D. G., Williams, P., Davies, M. C., & Alexander, M. R. (2012). Combinatorial discovery of polymers resistant to bacterial attachment. Nature Biotechnology, 30(9), 868-875. https://doi.org/10.1038/nbt.2316

Bacterial attachment and subsequent biofilm formation pose key challenges to the optimal performance of medical devices. In this study, we determined the attachment of selected bacterial species to hundreds of polymeric materials in a high-throughput... Read More about Combinatorial discovery of polymers resistant to bacterial attachment.

Methicillin resistance reduces the virulence of healthcare-associated methicillin-resistant staphylococcus aureus by interfering with the agr quorum sensing system (2012)
Journal Article
Rudkin, J. K., Edwards, A. M., Bowden, M. G., Brown, E. L., Pozzi, C., Waters, E. M., Chan, W. C., Williams, P., O'Gara, J. P., & Massey, R. C. (2012). Methicillin resistance reduces the virulence of healthcare-associated methicillin-resistant staphylococcus aureus by interfering with the agr quorum sensing system. Journal of Infectious Diseases, 205(5), 798-806. https://doi.org/10.1093/infdis/jir845

The difficulty in successfully treating infections caused by methicillin-resistant Staphylococcus aureus (MRSA) has led to them being referred to as highly virulent or pathogenic. In our study of one of the major healthcare-associated MRSA (HA-MRSA)... Read More about Methicillin resistance reduces the virulence of healthcare-associated methicillin-resistant staphylococcus aureus by interfering with the agr quorum sensing system.