LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules

Ortori, Catharine A.; Ortori, Catherine; Halliday, Nigel; C�mara, Miguel; Williams, Paul; Barrett, David A.

Abstract

© Springer Science+Business Media New York 2014. Extracts taken from spent growth media from Pseudomonas aeruginosa can be analyzed for N -acyl- L - homoserine lactones and 2-alkyl-4-(1 H)-quinolones (AQs), including the known quorum sensing signalling molecules of P. aeruginosa, in a specific and sensitive manner by liquid chromatography coupled with tandem mass spectrometric detection. This analysis can be conducted in a quantitative manner by comparison with matrix-matched calibration samples.

Citation

Ortori, C. A., Ortori, C., Halliday, N., Cámara, M., Williams, P., & Barrett, D. A. (2014). LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules. In A. Filloux, & J. Ramos (Eds.), Pseudomonas Methods and Protocols (255-270). Springer. https://doi.org/10.1007/978-1-4939-0473-0_21

• Acceptance Date: Jan 1, 2014
• Online Publication Date: Mar 29, 2014
• Publication Date: 2014
• Deposit Date: Jun 27, 2018
• Electronic ISSN: 1940-6029
• Publisher: Springer
• Peer Reviewed: Peer Reviewed
• Volume: 1149
• Pages: 255-270
• Book Title: Pseudomonas Methods and Protocols
• ISBN: 9781493904723
• DOI: https://doi.org/10.1007/978-1-4939-0473-0_21
• Public URL: https://nottingham-repository.worktribe.com/output/1098332
• Publisher URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-0473-0_21
• PMID: 24818911