Combinatorial discovery of polymers resistant to bacterial attachment

Hook, Andrew L.; Chang, Chien-Yi; Yang, Jing; Luckett, Jeni; Cockayne, Alan; Atkinson, Steve; Mei, Ying; Bayston, Roger; Irvine, Derek J.; Langer, Robert; Anderson, Daniel G.; Williams, Paul; Davies, Martyn C.; Alexander, Morgan R.

Abstract

Bacterial attachment and subsequent biofilm formation pose key challenges to the optimal performance of medical devices. In this study, we determined the attachment of selected bacterial species to hundreds of polymeric materials in a high-throughput microarray format. Using this method, we identified a group of structurally related materials comprising ester and cyclic hydrocarbon moieties that substantially reduced the attachment of pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli). Coating silicone with these 'hit' materials achieved up to a 30-fold (96.7%) reduction in the surface area covered by bacteria compared with a commercial silver hydrogel coating in vitro, and the same material coatings were effective at reducing bacterial attachment in vivo in a mouse implant infection model. These polymers represent a class of materials that reduce the attachment of bacteria that could not have been predicted to have this property from the current understanding of bacteria-surface interactions. © 2012 Nature America, Inc. All rights reserved.

Citation

Hook, A. L., Chang, C.-Y., Yang, J., Luckett, J., Cockayne, A., Atkinson, S., Mei, Y., Bayston, R., Irvine, D. J., Langer, R., Anderson, D. G., Williams, P., Davies, M. C., & Alexander, M. R. (2012). Combinatorial discovery of polymers resistant to bacterial attachment. Nature Biotechnology, 30(9), 868-875. https://doi.org/10.1038/nbt.2316

• Journal Article Type: Article
• Acceptance Date: Jun 26, 2012
• Online Publication Date: Aug 12, 2012
• Publication Date: 2012-09
• Deposit Date: Mar 3, 2016
• Publicly Available Date: Mar 3, 2016
• Journal: Nature Biotechnology
• Print ISSN: 1087-0156
• Electronic ISSN: 1546-1696
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 30
• Issue: 9
• Pages: 868-875
• DOI: https://doi.org/10.1038/nbt.2316
• Keywords: Bacterial Adhesion, Biomedical Materials, Polymer Synthesis
• Public URL: https://nottingham-repository.worktribe.com/output/710965
• Publisher URL: http://www.nature.com/nbt/journal/v30/n9/full/nbt.2316.html