Combinatorial discovery of polymers resistant to bacterial attachment

Hook, Andrew L.; Chang, Chien-Yi; Yang, Jing; Luckett, Jeni; Cockayne, Alan; Atkinson, Steve; Mei, Ying; Bayston, Roger; Irvine, Derek J.; Langer, Robert; Anderson, Daniel G.; Williams, Paul; Davies, Martyn C.; Alexander, Morgan R.

doi:10.1038/nbt.2316

Combinatorial discovery of polymers resistant to bacterial attachment

Hook, Andrew L.; Chang, Chien-Yi; Yang, Jing; Luckett, Jeni; Cockayne, Alan; Atkinson, Steve; Mei, Ying; Bayston, Roger; Irvine, Derek J.; Langer, Robert; Anderson, Daniel G.; Williams, Paul; Davies, Martyn C.; Alexander, Morgan R.

Authors

ANDREW HOOK ANDREW.HOOK@NOTTINGHAM.AC.UK
Assistant Professor

Chien-Yi Chang

JING YANG JING.YANG@NOTTINGHAM.AC.UK
Assistant Professor

JENI LUCKETT JENI.LUCKETT@NOTTINGHAM.AC.UK
Senior Research Fellow

Alan Cockayne

STEVE ATKINSON steve.atkinson@nottingham.ac.uk
Associate Professor

Ying Mei

Roger Bayston

Derek J. Irvine

Robert Langer

Daniel G. Anderson

PAUL WILLIAMS paul.williams@nottingham.ac.uk
Professor of Molecular Microbiology

Martyn C. Davies

MORGAN ALEXANDER morgan.alexander@nottingham.ac.uk
Professor of Biomedical Surfaces

Abstract

Bacterial attachment and subsequent biofilm formation pose key challenges to the optimal performance of medical devices. In this study, we determined the attachment of selected bacterial species to hundreds of polymeric materials in a high-throughput microarray format. Using this method, we identified a group of structurally related materials comprising ester and cyclic hydrocarbon moieties that substantially reduced the attachment of pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli). Coating silicone with these 'hit' materials achieved up to a 30-fold (96.7%) reduction in the surface area covered by bacteria compared with a commercial silver hydrogel coating in vitro, and the same material coatings were effective at reducing bacterial attachment in vivo in a mouse implant infection model. These polymers represent a class of materials that reduce the attachment of bacteria that could not have been predicted to have this property from the current understanding of bacteria-surface interactions. © 2012 Nature America, Inc. All rights reserved.

Citation

Hook, A. L., Chang, C., Yang, J., Luckett, J., Cockayne, A., Atkinson, S., …Alexander, M. R. (2012). Combinatorial discovery of polymers resistant to bacterial attachment. Nature Biotechnology, 30(9), 868-875. https://doi.org/10.1038/nbt.2316

Journal Article Type	Article
Acceptance Date	Jun 26, 2012
Online Publication Date	Aug 12, 2012
Publication Date	2012-09
Deposit Date	Mar 3, 2016
Publicly Available Date	Mar 3, 2016
Journal	Nature Biotechnology
Print ISSN	1087-0156
Electronic ISSN	1546-1696
Publisher	Nature Publishing Group
Peer Reviewed	Peer Reviewed
Volume	30
Issue	9
Pages	868-875
DOI	https://doi.org/10.1038/nbt.2316
Keywords	Bacterial Adhesion, Biomedical Materials, Polymer Synthesis
Public URL	https://nottingham-repository.worktribe.com/output/710965
Publisher URL	http://www.nature.com/nbt/journal/v30/n9/full/nbt.2316.html