Research Repository

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Anthropogenic environmental drivers of antimicrobial resistance in wildlife (2018)
Journal Article
Swift, B. M., Bennett, M., Waller, K., Dodd, C., Murray, A., Gomes, R. L., …Arnold, K. E. (2019). Anthropogenic environmental drivers of antimicrobial resistance in wildlife. Science of the Total Environment, 649, 12-20. doi:10.1016/j.scitotenv.2018.08.180

The isolation of antimicrobial resistant bacteria (ARB) from wildlife living adjacent to humans has led to the suggestion that such antimicrobial resistance (AMR) is anthropogenically driven by exposure to antimicrobials and ARB. However, ARB have al... Read More

Comparative genomics of bacteriophage of the genus Seuratvirus (2017)
Journal Article
Sazinas, P., Redgwell, T., Rihtman, B., Grigonyte, A., Michniewski, S., Scanlan, D. J., …Millard, A. (in press). Comparative genomics of bacteriophage of the genus Seuratvirus. Genome Biology and Evolution, 10(1), doi:10.1093/gbe/evx275. ISSN 1759-6653

Despite being more abundant and having smaller genomes than their bacterial host, relatively few bacteriophages have had their genomes sequenced. Here, we isolated 14 bacteriophages from cattle slurry and performed de novo genome sequencing, assembly... Read More

Bacterial resistance to arsenic protects against protist killing (2017)
Journal Article
Hao, X., Li, X., Pal, C., Hobman, J. L., Larsson, D. J., Saquib, Q., …Rensing, C. (2017). Bacterial resistance to arsenic protects against protist killing. BioMetals, 30(2), doi:10.1007/s10534-017-0003-4. ISSN 0966-0844

Protists kill their bacterial prey using toxic metals such as copper. Here we hypothesize that the metalloid arsenic has a similar role. To test this hypothesis, we examined intracellular survival of Escherichia coli (E. coli) in the amoeba Dictyoste... Read More

Draft genome sequence of the bacteriophage vB_Eco_slurp01. (2016)
Journal Article
Sazinas, P., Smith, C., Suhaimi, A., Hobman, J. L., Dodd, C. E., & Millard, A. (2016). Draft genome sequence of the bacteriophage vB_Eco_slurp01. Genome Announcements, 4(6), doi:10.1128/genomeA.01111-16. ISSN 2169-8287

Bacteriophage vB_Eco_slurp01 was isolated from porcine feces using Escherichia coli MG1655 as a host. With a genome size of 348 kb, vB_Eco_slurp01 is one of the largest bacteriophages isolated to date.

Mathematical modelling of antimicrobial resistance in agricultural waste highlights importance of gene transfer rate (2016)
Journal Article
Baker, M., Hobman, J. L., Dodd, C. E., Ramsden, S. J., & Stekel, D. J. (2016). Mathematical modelling of antimicrobial resistance in agricultural waste highlights importance of gene transfer rate. FEMS Microbiology Ecology, 92(4), doi:10.1093/femsec/fiw040. ISSN 0168-6496

Antimicrobial resistance is of global concern. Most antimicrobial use is in agriculture; manures and slurry are especially important because they contain a mix of bacteria, including potential pathogens, antimicrobial resistance genes and antimicrobi... Read More

Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm (2016)
Journal Article
Ibrahim, D., Dodd, C. E., Stekel, D. J., Ramsden, S., & Hobman, J. L. (2016). Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm. FEMS Microbiology Ecology, 92(4), doi:10.1093/femsec/fiw013. ISSN 0168-6496

Escherichia coli strains were isolated from a single dairy farm as a sentinel organism for the persistence of antibiotic resistance genes in the farm environment. Selective microbiological media were used to isolate 126 E. coli isolates from slurry a... Read More

Draft genome sequences of 14 Escherichia coli phages isolated from cattle slurry (2015)
Journal Article
Smith, R., O'Hara, M., Hobman, J. L., & Millard, A. (2015). Draft genome sequences of 14 Escherichia coli phages isolated from cattle slurry. Genome Announcements, 3(6), doi:10.1128/genomeA.01364-15. ISSN 2169-8287

The diversity of bacteriophages in slurry from dairy cows remains largely unknown. Here, we report the draft genome sequences of 14 bacteriophages isolated from dairy cow slurry using Escherichia coli K-12 MG1655 as a host.

The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress (2015)
Journal Article
Takahashi, H., Oshima, T., Hobman, J. L., Doherty, N., Clayton, S. R., Iqbal, M., …Stekel, D. J. (2015). The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress. Interface, 12(106), doi:10.1098/rsif.2015.0069

Zinc is essential for life, but toxic in excess. Thus all cells must control their internal zinc concentration. We used a systems approach, alternating rounds of experiments and models, to further elucidate the zinc control systems in Escherichia col... Read More

Response of Cupriavidus metallidurans CH34 to metals (2015)
Book
Monsieurs, P., Hobman, J. L., Vandenbussche, G., Mergeay, M., & Van Hoult, R. (2015). Response of Cupriavidus metallidurans CH34 to metals. In M. Mergay, & R. Van Houdt (Eds.), Metal response in Cupriavidus metallidurans Volume 1: From habitats to genes and proteinsSpringer. doi:10.1007/978-3-319-20594-6

Cupriavidus metallidurans CH34 displays resistance to a plethora of metals. Its response and underlying genetic determinants are dissected and detailed metal by metal (from arsenic to zinc). An important role for its megaplasmids pMOL28 and pMOL30 is... Read More