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All Outputs (24)

Bioinspired Precision Engineering of Three-Dimensional Epithelial Stem Cell Microniches (2020)
Journal Article
Prina, E., Amer, M. H., Sidney, L., Tromayer, M., Moore, J., Liska, R., …Rose, F. R. (2020). Bioinspired Precision Engineering of Three-Dimensional Epithelial Stem Cell Microniches. Advanced Biosystems, 4(6), Article 2000016. https://doi.org/10.1002/adbi.202000016

© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Maintenance of the epithelium relies on stem cells residing within specialized microenvironments, known as epithelial crypts. Two-photon polymerization (2PP) is a valuable... Read More about Bioinspired Precision Engineering of Three-Dimensional Epithelial Stem Cell Microniches.

Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane (2019)
Journal Article
Marsit, N. M., Sidney, L. E., Britchford, E. R., McIntosh, O. D., Allen, C. L., Ashraf, W., …Hopkinson, A. (2019). Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane. Scientific Reports, 9(1), Article 12854. https://doi.org/10.1038/s41598-019-49314-7

© 2019, The Author(s). Amniotic membrane (AM) is used to treat a range of ophthalmic indications but must be presented in a non-contaminated state. AM from elective caesarean sections contains natural microbial contamination, requiring removal during... Read More about Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane.

Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model (2019)
Journal Article
Morales, M. L. O., Marsit, N. M., McIntosh, O. D., Hopkinson, A., & Sidney, L. E. (2019). Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model. World Journal of Stem Cells, 11(2), 84-99. https://doi.org/10.4252/wjsc.v11.i2.84

BACKGROUND An in vitro injury model mimicking a corneal surface injury was optimised using human corneal epithelial cells (hCEC). AIM To investigate whether corneal-stroma derived stem cells (CSSC) seeded on an amniotic membrane (AM) construct m... Read More about Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model.

A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells (2019)
Journal Article
Aladdad, A. M., Amer, M. H., Sidney, L., Hopkinson, A., White, L. J., Alexander, C., & Rose, F. R. (2019). A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells. Acta Biomaterialia, 95, 427-438. https://doi.org/10.1016/j.actbio.2019.01.037

A three-dimensional thermoresponsive fibrous scaffold system for the subsequent extended culture and enzyme-free passaging of a range of mammalian cell types is presented. Poly(PEGMA188) was incorporated with poly(ethylene terephthalate) (PET) via bl... Read More about A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells.

In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft (2018)
Journal Article
Rose, J., Sidney, L., Patient, J., White, L., Dua, H., El Haj, A., …Rose, F. (2018). In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft. Journal of Biomedical Materials Research Part A, 107(4), 828-838. https://doi.org/10.1002/jbm.a.36598

The advent of innovative surgical procedures utilizing partial thickness corneal grafts has created a need for the development of synthetic implants to recreate corneal stromal tissue. This work evaluates electrospun gelatin and polycaprolactone (PCL... Read More about In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft.

3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review (2017)
Journal Article
Prina, E., Mistry, P., Sidney, L. E., Yang, J., Wildman, R. D., Bertolin, M., …Rose, F. R. A. (2017). 3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review. Stem Cell Reviews and Reports, 13(3), 430-441. https://doi.org/10.1007/s12015-017-9740-6

In recent years, there has been increased research interest in generating corneal substitutes, either for use in the clinic or as in vitro corneal models. The advancement of 3D microfabrication technologies has allowed the reconstruction of the nativ... Read More about 3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review.

Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells (2016)
Journal Article
Nagymihály, R., Veréb, Z., Albert, R., Sidney, L., Dua, H., Hopkinson, A., & Petrovski, G. (2017). Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells. Clinical and Experimental Ophthalmology, 45(5), 509-519. https://doi.org/10.1111/ceo.12903

Background The study aims to characterise human corneal endothelial cell (HCEnC) cultures generated by the peel?and?digest method based on their surface protein/carbohydrate expression pattern. Methods Quantitative polymerase chain reaction was... Read More about Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells.

Terminal sterilization: conventional methods versus emerging cold atmospheric pressure plasma technology for non-viable biological tissues (2016)
Journal Article
Marsit, N. M., Sidney, L. E., Branch, M. J., Wilson, S. L., & Hopkinson, A. (in press). Terminal sterilization: conventional methods versus emerging cold atmospheric pressure plasma technology for non-viable biological tissues. Plasma Processes and Polymers, https://doi.org/10.1002/ppap.201600134

Tissue products are susceptible to microbial contamination from different sources, which may cause disease transmission upon transplantation. Terminal sterilization using gamma radiation, electron-beam, and ethylene oxide protocols are well-establish... Read More about Terminal sterilization: conventional methods versus emerging cold atmospheric pressure plasma technology for non-viable biological tissues.

Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells (2015)
Journal Article
Sidney, L. E., Branch, M. J., Dua, H. S., & Hopkinson, A. (2015). Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells. Cytotherapy, 17(12), https://doi.org/10.1016/j.jcyt.2015.08.003

Background: The limbal area of the corneal stroma has been identified as a source of mesenchymal-like stem cells, which have potential for exploitation as a cell therapy. However, the optimal culture conditions are disputed and few direct media compa... Read More about Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells.

Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells (2015)
Journal Article
Sidney, L. E., McIntosh, O. D., & Hopkinson, A. (2015). Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells. Investigative Ophthalmology & Visual Science, 56(12), https://doi.org/10.1167/iovs.15-17810

Purpose: Cells of the corneal epithelium and stroma can be distinguished in vivo by different intermediate filaments, cytokeratins for corneal epithelial cells (CEC) and vimentin for keratocytes. Isolated and cultured keratocytes change phenotype, lo... Read More about Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells.

Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation? (2015)
Journal Article
Wilson, S. L., Sidney, L. E., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2016). Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?. Current Eye Research, 41(6), 769-782. https://doi.org/10.3109/02713683.2015.1062114

Background: There is a clinical need for biomimetic corneas that are as effective, preferably superior, to cadaveric donor tissue. Decellularized tissues are advantageous compared to synthetic or semi-synthetic engineered tissues in that the native m... Read More about Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?.

In vitro studies on the antimicrobial peptide human beta-defensin 9 (HBD9): signalling pathways and pathogen-related response (an American Ophthalmological Society thesis) (2014)
Journal Article
Dua, H. S., Otri, A. M., Hopkinson, A., & Mohammed, I. (2014). In vitro studies on the antimicrobial peptide human beta-defensin 9 (HBD9): signalling pathways and pathogen-related response (an American Ophthalmological Society thesis). Transactions of the American Ophthalmological Society, 112, 50-73

Purpose: Human β-defensins (HBDs) are an important part of the innate immune host defense at the ocular surface. Unlike other defensins, expression of HBD9 at the ocular surface is reduced during microbial infection, but activation of toll-like recep... Read More about In vitro studies on the antimicrobial peptide human beta-defensin 9 (HBD9): signalling pathways and pathogen-related response (an American Ophthalmological Society thesis).

Concise review: evidence for CD34 as a common marker for diverse progenitors (2014)
Journal Article
Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2014). Concise review: evidence for CD34 as a common marker for diverse progenitors. STEM CELLS, 32(6), 1380-1389. https://doi.org/10.1002/stem.1661

CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical... Read More about Concise review: evidence for CD34 as a common marker for diverse progenitors.

Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies (2013)
Journal Article
Kulkarn, B. B. .., Powe, D. G., Hopkinson, A., & Dua, H. S. (2013). Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies. BMC Ophthalmology, 13, Article 62. https://doi.org/10.1186/1471-2415-13-62

Background The most important challenge of performing insitu transcriptional profiling of the human ocular surface epithelial regions is obtaining samples in sufficient amounts, without contamination from adjacent tissue, as the region of interest i... Read More about Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies.

Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation (2013)
Journal Article
Hashmani, K., Branch, M. J., Sidney, L. E., Dhillon, P. S., Verma, M., McIntosh, O. D., …Dua, H. S. (2013). Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation. Stem Cell Research and Therapy, 4(3), Article 75. https://doi.org/10.1186/scrt226

Introduction: The corneal stroma is being increasingly recognized as a repository for stem cells. Like the limbal and endothelial niches, stromal stem cells often reside in the peripheral cornea and limbus. These peripheral and limbal corneal stromal... Read More about Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation.

Increased Expression of Hepcidin and Toll-Like Receptors 8 and 10 in Viral Keratitis (2011)
Journal Article
Mohammed, I., Abedin, A., Tsintzas, K., Abedin, S. A., Otri, A. M., Hopkinson, A., …Dua, H. S. (2011). Increased Expression of Hepcidin and Toll-Like Receptors 8 and 10 in Viral Keratitis. Cornea, 30(8), 899-904. https://doi.org/10.1097/ICO.0b013e31820126e5

Purpose: Antimicrobial peptides (AMPs) and toll-like receptors (TLRs) form part of the “chemical barrier” of the ocular surface to microbes. Evidence suggests that pathogen recognition by TLR releases AMPs, altering AMP-TLR profiles in pathologic... Read More about Increased Expression of Hepcidin and Toll-Like Receptors 8 and 10 in Viral Keratitis.

Signalling pathways involved in ribonuclease-7 expression (2010)
Journal Article
Mohammed, I., Yeung, A., Abedin, A., Hopkinson, A., & Dua, H. S. (2011). Signalling pathways involved in ribonuclease-7 expression. Cellular and Molecular Life Sciences, 68(11), 1941-1952. https://doi.org/10.1007/s00018-010-0540-2

Antimicrobial peptides are host defence molecules that play a potential role in preventing infection at the epithelial surfaces. Ribonuclease (RNase)-7 has been shown to possess a broad spectrum of microbicidal activity against various pathogens. Her... Read More about Signalling pathways involved in ribonuclease-7 expression.

Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics (2010)
Journal Article
Kulkarni, B. B., Tighe, P. J., Mohammed, I., Yeung, A. M., Powe, D. G., Hopkinson, A., …Dua, H. S. (2010). Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics. BMC Genomics, 11(1), Article 526. https://doi.org/10.1186/1471-2164-11-526

Background: The Limbal epithelial crypt (LEC) is a solid cord of cells, approximately 120 microns long. It arises from the undersurface of interpalisade rete ridges of the limbal palisades of Vogt and extends deeper into the limbal stroma parallel or... Read More about Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics.