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A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells

Aladdad, Afnan M.; Amer, Mahetab H.; Sidney, Laura; Hopkinson, Andrew; White, Lisa J.; Alexander, Cameron; Rose, Felicity R.A.J.

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Authors

Afnan M. Aladdad

Mahetab H. Amer

LAURA SIDNEY LAURA.SIDNEY@NOTTINGHAM.AC.UK
Senior Research Fellow

Andrew Hopkinson

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FELICITY ROSE FELICITY.ROSE@NOTTINGHAM.AC.UK
Professor of Biomaterials and Tissue Engineering



Abstract

A three-dimensional thermoresponsive fibrous scaffold system for the subsequent extended culture and enzyme-free passaging of a range of mammalian cell types is presented. Poly(PEGMA188) was incorporated with poly(ethylene terephthalate) (PET) via blend-electrospinning to render the fibre thermoresponsive. Using primary human corneal stromal stem cells as an therapeutically relevant exemplar, cell adhesion, viability, proliferation and phenotype on this fibrous culture system over numerous thermal enzyme-free passages is described. We also illustrate the versatility of this system with respect to fabricating thermoresponsive fibres from biodegradable polymers and for the culture of diverse mammalian cell types including mesenchymal stem cells, colon adenocarcinoma cells and NIH-3T3 fibroblasts. This thermoresponsive scaffold system combines the advantages of providing a physiologically relevant environment to maintain a desirable cell phenotype, allowing routine enzyme-free passaging and expansion of cultured cells, whilst offering mechanical support for cell growth. The system described in this study presents a versatile platform for biomedical applications and more specifically for the expansion of mammalian cells destined for the clinic.

Citation

Aladdad, A. M., Amer, M. H., Sidney, L., Hopkinson, A., White, L. J., Alexander, C., & Rose, F. R. (2019). A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells. Acta Biomaterialia, 95, 427-438. https://doi.org/10.1016/j.actbio.2019.01.037

Journal Article Type Article
Acceptance Date Jan 17, 2019
Online Publication Date Jan 19, 2019
Publication Date Sep 1, 2019
Deposit Date Apr 3, 2019
Publicly Available Date Apr 3, 2019
Journal Acta Biomaterialia
Print ISSN 1742-7061
Electronic ISSN 1878-7568
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 95
Pages 427-438
DOI https://doi.org/10.1016/j.actbio.2019.01.037
Keywords Biotechnology; Biochemistry; Molecular Biology; Biomaterials; Biomedical Engineering; General Medicine
Public URL https://nottingham-repository.worktribe.com/output/1585371
Publisher URL https://www.sciencedirect.com/science/article/pii/S1742706119300571

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