Afnan M. Aladdad
A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells
Aladdad, Afnan M.; Amer, Mahetab H.; Sidney, Laura; Hopkinson, Andrew; White, Lisa J.; Alexander, Cameron; Rose, Felicity R.A.J.
Mahetab H. Amer
Lisa J. White
Felicity R.A.J. Rose
A three-dimensional thermoresponsive fibrous scaffold system for the subsequent extended culture and enzyme-free passaging of a range of mammalian cell types is presented. Poly(PEGMA188) was incorporated with poly(ethylene terephthalate) (PET) via blend-electrospinning to render the fibre thermoresponsive. Using primary human corneal stromal stem cells as an therapeutically relevant exemplar, cell adhesion, viability, proliferation and phenotype on this fibrous culture system over numerous thermal enzyme-free passages is described. We also illustrate the versatility of this system with respect to fabricating thermoresponsive fibres from biodegradable polymers and for the culture of diverse mammalian cell types including mesenchymal stem cells, colon adenocarcinoma cells and NIH-3T3 fibroblasts. This thermoresponsive scaffold system combines the advantages of providing a physiologically relevant environment to maintain a desirable cell phenotype, allowing routine enzyme-free passaging and expansion of cultured cells, whilst offering mechanical support for cell growth. The system described in this study presents a versatile platform for biomedical applications and more specifically for the expansion of mammalian cells destined for the clinic.
|Journal Article Type||Article|
|Publication Date||Sep 1, 2019|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Aladdad, A. M., Amer, M. H., Sidney, L., Hopkinson, A., White, L. J., Alexander, C., & Rose, F. R. (2019). A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells. Acta Biomaterialia, 95, 427-438. doi:10.1016/j.actbio.2019.01.037|
|Keywords||Biotechnology; Biochemistry; Molecular Biology; Biomaterials; Biomedical Engineering; General Medicine|
Aladdad et al 2018_AB-18-1894_REVISIONS2_MANUSCPT_FINAL