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A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells

Aladdad, Afnan M.; Amer, Mahetab H.; Sidney, Laura; Hopkinson, Andrew; White, Lisa J.; Alexander, Cameron; Rose, Felicity R.A.J.


Afnan M. Aladdad

Mahetab H. Amer

Laura Sidney

Andrew Hopkinson

Lisa J. White

Cameron Alexander

Felicity R.A.J. Rose


A three-dimensional thermoresponsive fibrous scaffold system for the subsequent extended culture and enzyme-free passaging of a range of mammalian cell types is presented. Poly(PEGMA188) was incorporated with poly(ethylene terephthalate) (PET) via blend-electrospinning to render the fibre thermoresponsive. Using primary human corneal stromal stem cells as an therapeutically relevant exemplar, cell adhesion, viability, proliferation and phenotype on this fibrous culture system over numerous thermal enzyme-free passages is described. We also illustrate the versatility of this system with respect to fabricating thermoresponsive fibres from biodegradable polymers and for the culture of diverse mammalian cell types including mesenchymal stem cells, colon adenocarcinoma cells and NIH-3T3 fibroblasts. This thermoresponsive scaffold system combines the advantages of providing a physiologically relevant environment to maintain a desirable cell phenotype, allowing routine enzyme-free passaging and expansion of cultured cells, whilst offering mechanical support for cell growth. The system described in this study presents a versatile platform for biomedical applications and more specifically for the expansion of mammalian cells destined for the clinic.

Journal Article Type Article
Publication Date Sep 1, 2019
Journal Acta Biomaterialia
Print ISSN 1742-7061
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 95
Pages 427-438
APA6 Citation Aladdad, A. M., Amer, M. H., Sidney, L., Hopkinson, A., White, L. J., Alexander, C., & Rose, F. R. (2019). A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells. Acta Biomaterialia, 95, 427-438. doi:10.1016/j.actbio.2019.01.037
Keywords Biotechnology; Biochemistry; Molecular Biology; Biomaterials; Biomedical Engineering; General Medicine
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