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In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft

Rose, J.B.; Sidney, L.E.; Patient, J.; White, L.J.; Dua, H.S.; El Haj, A.J.; Hopkinson, A.; Rose, F.R.A.J.

In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft Thumbnail


Authors

J.B. Rose

LAURA SIDNEY LAURA.SIDNEY@NOTTINGHAM.AC.UK
Senior Research Fellow

J. Patient

HARMINDER DUA HARMINDER.DUA@NOTTINGHAM.AC.UK
Professor of Ophthalmology and Visual Sciences

A.J. El Haj

A. Hopkinson

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FELICITY ROSE FELICITY.ROSE@NOTTINGHAM.AC.UK
Professor of Biomaterials and Tissue Engineering



Abstract

The advent of innovative surgical procedures utilizing partial thickness corneal grafts has created a need for the development of synthetic implants to recreate corneal stromal tissue. This work evaluates electrospun gelatin and polycaprolactone (PCL) scaffolds as a potential biomaterial suitable for use in regeneration of corneal stromal tissue. Electrospun gelatin has been used for many years in tissue engineering, however, post‐production modification, such as crosslinking, is usually required to mechanically strengthen such scaffolds. This paper aims therefore to compare glutaraldehyde (GA) cross‐linked electrospun gelatin scaffolds with electrospun blends of gelatin and PCL at different ratios. Scaffolds were fabricated using electrospinning and characterized by scanning electron microscopy, Attenuated Total Reflectance‐Fourier Transform Infrared Spectroscopy (ATR‐FTIR), and tensile testing. To evaluate biocompatibility, primary human corneal stromal cells (hCSC) were seeded upon the scaffolds to assess adherence, proliferation and phenotype. Results demonstrated that scaffolds fabricated from mixtures of gelatin and PCL showed increased mechanical strength and plasticity compared to scaffolds fabricated from GA cross‐linked gelatin alone. In addition, scaffolds fabricated from PCL and gelatin showed comparable support of hCSC adhesion and proliferation. In conclusion, blended mixtures of gelatin and PCL can be considered as an option in the selection of corneal repair materials in the future.

Journal Article Type Article
Acceptance Date Dec 5, 2018
Online Publication Date Dec 22, 2018
Publication Date Dec 22, 2018
Deposit Date Jan 15, 2019
Publicly Available Date Dec 23, 2019
Journal Journal of Biomedical Materials Research Part A
Print ISSN 1549-3296
Electronic ISSN 1552-4965
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 107
Issue 4
Pages 828-838
DOI https://doi.org/10.1002/jbm.a.36598
Keywords Metals and alloys; Biomaterials; Ceramics and composites; Biomedical engineering
Public URL https://nottingham-repository.worktribe.com/output/1473458
Publisher URL https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.36598
Additional Information This is the peer reviewed version of the following article: Rose, J. B., Sidney, L. E., Patient, J. , White, L. J., Dua, H. S., El Haj, A. J., Hopkinson, A. and Rose, F. R. (2019), In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft. J Biomed Mater Res. which has been published in final form at |https://doi.org/10.1002/jbm.a.36598. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

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