Mariana Lizeth Orozco Morales
Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model
Morales, Mariana Lizeth Orozco; Marsit, Nagi M.; McIntosh, Owen D.; Hopkinson, Andrew; Sidney, Laura E.
Nagi M. Marsit
Owen D. McIntosh
LAURA SIDNEY Laura.Sidney@nottingham.ac.uk
Senior Research Fellow
An in vitro injury model mimicking a corneal surface injury was optimised using human corneal epithelial cells (hCEC).
To investigate whether corneal-stroma derived stem cells (CSSC) seeded on an amniotic membrane (AM) construct manifests an anti-inflammatory, healing response.
Treatment of hCEC with ethanol and pro-inflammatory cytokines were compared in terms of viability loss, cytotoxicity, and pro-inflammatory cytokine release, in order to generate the in vitro injury. This resulted in an optimal injury of 20% (v/v) ethanol for 30 s with 1 ng/mL interleukin-1 (IL-1) beta. Co-culture experiments were performed with CSSC alone and with CSSC-AM constructs. The effect of injury and co-culture on viability, cytotoxicity, IL-6 and IL-8 production, and IL1B, TNF, IL6, and CXCL8 mRNA expression were assessed.
Co-culture with CSSC inhibited loss of hCEC viability caused by injury. Enzyme linked immunosorbent assay and polymerase chain reaction showed a significant reduction in the production of IL-6 and IL-8 pro-inflammatory cytokines, and reduction in pro-inflammatory cytokine mRNA expression during co-culture with CSSC alone and with the AM construct. These results confirmed the therapeutic potential of the CSSC and the possible use of AM as a cell carrier for application to the ocular surface.
CSSC were shown to have a potentially therapeutic anti-inflammatory effect when treating injured hCEC, demonstrating an important role in corneal regeneration and wound healing, leading to an improved knowledge of their potential use for research and therapeutic purposes.
|Journal Article Type||Article|
|Publication Date||Feb 26, 2019|
|Journal||World Journal of Stem Cells|
|Publisher||Baishideng Publishing Group|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Morales, M. L. O., Marsit, N. M., McIntosh, O. D., Hopkinson, A., & Sidney, L. E. (2019). Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model. World Journal of Stem Cells, 11(2), 84-99. https://doi.org/10.4252/wjsc.v11.i2.84|
|Keywords||Cornea; Corneal injuries; Injury model; Corneal epithelium; Corneal stromaderived stem cells; Amnion; Anti-inflammatory; Cell therapy|
Orozco Et Al.
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