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Accelerated 19F·MRI Detection of Matrix Metalloproteinase-2/-9 through Responsive Deactivation of Paramagnetic Relaxation Enhancement

Faas, Henryk M.; Krupa, James L.; Taylor, Alexander J.; Zamberlan, Francesco; Philp, Christopher J.; Williams, Huw E. L.; Johnson, Simon R.; Pavlovskaya, Galina E.; Thomas, Neil R.; Meersmann, Thomas

Authors

Henryk M. Faas

James L. Krupa

Alexander J. Taylor

Francesco Zamberlan

Christopher J. Philp

Huw E. L. Williams

Simon R. Johnson

Galina E. Pavlovskaya

Neil R. Thomas

Thomas Meersmann



Abstract

Paramagnetic gadolinium ions (Gd III), complexed within DOTA-based chelates, have become useful tools to increase the magnetic resonance imaging (MRI) contrast in tissues of interest. Recently, " on/oo " probes serving as 19 F·MRI biosensors for target enzymes have emerged that utilize the increase in transverse (T * 2 or T 2) relaxation times upon cleavage of the paramagnetic Gd III centre. Molecular 19 F·MRI has the advantage of high speciicity due to the lack of background signal but suuers from low signal intensity that leads to low spatial resolution and long recording times. In this work, an " on/oo " probe concept is introduced that utilizes responsive deactivation of paramagnetic relaxation enhancement (PRE) to generate 19 F longitudinal (T 1) relaxation contrast for accelerated molecular MRI. e probe concept is applied to matrix metalloproteinases (MMPs), a class of enzymes linked with many innammatory diseases and cancer that modify bioactive extracellular substrates. e presence of these bio-markers in extracellular space makes MMPs an accessible target for responsive PRE deactivation probes. Responsive PRE deactivation in a 19 F biosensor probe, selective for MMP-2 and MMP-9, is shown to enable molecular MRI contrast at signiicantly reduced experimental times compared to previous methods. PRE deactivation was caused by MMP through cleavage of a protease substrate that served as a linker between the uorine-containing moiety and a paramagnetic Gd III-bound DOTA complex. Ultrashort echo time (UTE) MRI and, alternatively, short echo times in standard gradient echo (GE) MRI were employed to cope with the fast 19 F transverse relaxation of the PRE active probe in its " on-state. " Upon responsive PRE deactivation, the 19 F·MRI signal from the " oo-state " probe diminished, thereby indicating the presence of the target enzyme through the associated negative MRI contrast. Null point 1 H·MRI, obtainable within a short time course, was employed to identify false-positive 19 F·MRI responses caused by dilution of the contrast agent.

Journal Article Type Article
Publication Date Feb 28, 2019
Journal Contrast Media & Molecular Imaging
Print ISSN 1555-4309
Electronic ISSN 1555-4317
Publisher Hindawi Publishing Corporation
Peer Reviewed Peer Reviewed
Volume 2019
Article Number 4826520
Pages 1-13
APA6 Citation Faas, H. M., Krupa, J. L., Taylor, A. J., Zamberlan, F., Philp, C. J., Williams, H. E. L., …Meersmann, T. (2019). Accelerated 19F·MRI Detection of Matrix Metalloproteinase-2/-9 through Responsive Deactivation of Paramagnetic Relaxation Enhancement. Contrast Media and Molecular Imaging, 2019, 1-13. https://doi.org/10.1155/2019/4826520
DOI https://doi.org/10.1155/2019/4826520
Keywords Radiology Nuclear Medicine and imaging
Publisher URL https://www.hindawi.com/journals/cmmi/2019/4826520/

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