Professor MORGAN ALEXANDER's Outputs (136)

Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging (2023)
Journal Article
Linke, F., Johnson, J. E. C., Kern, S., Bennett, C. D., Lourdusamy, A., Lea, D., Clifford, S. C., Merry, C. L. R., Stolnik, S., Alexander, M. R., Peet, A. C., Scurr, D. J., Griffiths, R. L., Grabowska, A. M., Kerr, I. D., & Coyle, B. (2023). Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging. Acta Neuropathologica Communications, 11(1), Article 6. https://doi.org/10.1186/s40478-022-01496-4

The most common malignant brain tumour in children, medulloblastoma (MB), is subdivided into four clinically relevant molecular subgroups, although targeted therapy options informed by understanding of different cellular features are lacking. Here, b... Read More about Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging.

Predictive Molecular Design and Structure–Property Validation of Novel Terpene-Based, Sustainably Sourced Bacterial Biofilm-Resistant Materials (2023)
Journal Article
Cuzzucoli Crucitti, V., Ilchev, A., Moore, J. C., Fowler, H. R., Dubern, J.-F., Sanni, O., Xue, X., Husband, B. K., Dundas, A. A., Smith, S., Wildman, J. L., Taresco, V., Williams, P., Alexander, M. R., Howdle, S. M., Wildman, R. D., Stockman, R. A., & Irvine, D. J. (2023). Predictive Molecular Design and Structure–Property Validation of Novel Terpene-Based, Sustainably Sourced Bacterial Biofilm-Resistant Materials. Biomacromolecules, https://doi.org/10.1021/acs.biomac.2c00721

Presented in this work is the use of a molecular descriptor, termed the α parameter, to aid in the design of a series of novel, terpene-based, and sustainable polymers that were resistant to biofilm formation by the model bacterial pathogen Pseudomon... Read More about Predictive Molecular Design and Structure–Property Validation of Novel Terpene-Based, Sustainably Sourced Bacterial Biofilm-Resistant Materials.

Microparticles Decorated with Cell‐Instructive Surface Chemistries Actively Promote Wound Healing (2022)
Journal Article
Latif, A., Fisher, L. E., Dundas, A. A., Crucitti, V. C., Imir, Z., Lawler, K., Pappalard, F., Muir, B. W., Wildman, R., Irvine, D. J., Alexander, M. R., & Ghaemmaghami, A. M. (2024). Microparticles Decorated with Cell‐Instructive Surface Chemistries Actively Promote Wound Healing. Advanced Materials, 36(43), Article 2208364. https://doi.org/10.1002/adma.202208364

Wound healing is a complex biological process involving close crosstalk between various cell types. Dysregulation in any of these processes, such as in diabetic wounds, results in chronic nonhealing wounds. Fibroblasts are a critical cell type involv... Read More about Microparticles Decorated with Cell‐Instructive Surface Chemistries Actively Promote Wound Healing.

Evaluation of the relative potential for contact and doffing transmission of SARS-CoV-2 by a range of personal protective equipment materials (2022)
Journal Article
Xue, X., Coleman, C. M., Duncan, J. D., Hook, A. L., Ball, J. K., Alexander, C., & Alexander, M. R. (2022). Evaluation of the relative potential for contact and doffing transmission of SARS-CoV-2 by a range of personal protective equipment materials. Scientific Reports, 12(1), Article 16654. https://doi.org/10.1038/s41598-022-20952-8

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—the causative agent of coronavirus disease 2019 (COVID-19)—has caused a global public health emergency. Personal protective equipment (PPE) is the primary defence against viral exposure in... Read More about Evaluation of the relative potential for contact and doffing transmission of SARS-CoV-2 by a range of personal protective equipment materials.

Feature importance in machine learning models: A fuzzy information fusion approach (2022)
Journal Article
Rengasamy, D., Mase, J. M., Kumar, A., Rothwell, B., Torres, M. T., Alexander, M. R., Winkler, D. A., & Figueredo, G. P. (2022). Feature importance in machine learning models: A fuzzy information fusion approach. Neurocomputing, 511, 163-174. https://doi.org/10.1016/j.neucom.2022.09.053

With the widespread use of machine learning to support decision-making, it is increasingly important to verify and understand the reasons why a particular output is produced. Although post-training feature importance approaches assist this interpreta... Read More about Feature importance in machine learning models: A fuzzy information fusion approach.

Exploiting Generative Design for Multi-Material Inkjet 3D Printed Cell Instructive, Bacterial Biofilm Resistant Composites (2022)
Preprint / Working Paper
he, Y., Begines, B., Trindade, G., Abdi, M., dubern, J.-F., Prina, E., Hook, A., Choong, G., Ledesma, J., Tuck, C., R. A. J. Rose, F., Hague, R., Roberts, C., De Focatiis, D., Ashcroft, I., Williams, P., Irvine, D., alexander, M., & Wildman, R. Exploiting Generative Design for Multi-Material Inkjet 3D Printed Cell Instructive, Bacterial Biofilm Resistant Composites

As our understanding of disease grows, it is becoming established that treatment needs to be personalized and targeted to the needs of the individual. In this paper we show that multi-material inkjet-based 3D printing, when backed with generative des... Read More about Exploiting Generative Design for Multi-Material Inkjet 3D Printed Cell Instructive, Bacterial Biofilm Resistant Composites.

Single-Cell Metabolic Profiling of Macrophages Using 3D OrbiSIMS: Correlations with Phenotype (2022)
Journal Article
Suvannapruk, W., Edney, M. K., Kim, D.-H., Scurr, D. J., Ghaemmaghami, A. M., & Alexander, M. R. (2022). Single-Cell Metabolic Profiling of Macrophages Using 3D OrbiSIMS: Correlations with Phenotype. Analytical Chemistry, 94(26), 9389–9398. https://doi.org/10.1021/acs.analchem.2c01375

Macrophages are important immune cells that respond to environmental cues acquiring a range of activation statuses represented by pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes at each end of their spectrum. Characterizing the metabolic... Read More about Single-Cell Metabolic Profiling of Macrophages Using 3D OrbiSIMS: Correlations with Phenotype.

Utilising micron scale 3D printed morphologies for particle adhesion reduction (2022)
Journal Article
Marsh, G. E., Bunker, M. J., Alexander, M. R., Wildman, R. D., Nicholas, M., & Roberts, C. J. (2022). Utilising micron scale 3D printed morphologies for particle adhesion reduction. Powder Technology, 404, Article 117418. https://doi.org/10.1016/j.powtec.2022.117418

In the pharmaceutical industry, the ability to improve the understanding of the effect of surface roughness on interparticulate interactions is critical. Dry powder inhalers often possess poor efficiency, as the powder formulations are inherently adh... Read More about Utilising micron scale 3D printed morphologies for particle adhesion reduction.

Elucidating the molecular landscape of the stratum corneum (2022)
Journal Article
Starr, N. J., Khan, M. H., Edney, M. K., Trindade, G. F., Kern, S., Pirkl, A., Kleine-Boymann, M., Elmse, C., O'Mahony, M. M., Bell, M., Alexander, M. R., & Scurr, D. J. (2022). Elucidating the molecular landscape of the stratum corneum. Proceedings of the National Academy of Sciences, 119(12), Article e2114380119. https://doi.org/10.1073/pnas.2114380119

Characterization of the molecular structure of skin, especially the barrier layer, the stratum corneum, is a key research priority for generating understanding to improve diagnostics, aid pharmaceutical delivery, and prevent environmental damage. Our... Read More about Elucidating the molecular landscape of the stratum corneum.

Molecular Formula Prediction for Chemical Filtering of 3D OrbiSIMS Datasets (2022)
Journal Article
Edney, M. K., Kotowska, A. M., Spanu, M., Trindade, G. F., Wilmot, E., Reid, J., Barker, J., Aylott, J. W., Shard, A. G., Alexander, M. R., Snape, C. E., & Scurr, D. J. (2022). Molecular Formula Prediction for Chemical Filtering of 3D OrbiSIMS Datasets. Analytical Chemistry, 94(11), 4703–4711. https://doi.org/10.1021/acs.analchem.1c04898

Modern mass spectrometry techniques produce a wealth of spectral data, and although this is an advantage in terms of the richness of the information available, the volume and complexity of data can prevent a thorough interpretation to reach useful co... Read More about Molecular Formula Prediction for Chemical Filtering of 3D OrbiSIMS Datasets.

Efficacy of antimicrobial and anti-viral coated air filters to prevent the spread of airborne pathogens (2022)
Journal Article
Watson, R., Oldfield, M., Bryant, J. A., Riordan, L., Hill, H. J., Watts, J. A., Alexander, M. R., Cox, M. J., Stamataki, Z., Scurr, D. J., & de Cogan, F. (2022). Efficacy of antimicrobial and anti-viral coated air filters to prevent the spread of airborne pathogens. Scientific Reports, 12(1), Article 2803. https://doi.org/10.1038/s41598-022-06579-9

The COVID-19 pandemic has demonstrated the real need for mechanisms to control the spread of airborne respiratory pathogens. Thus, preventing the spread of disease from pathogens has come to the forefront of the public consciousness. This has brought... Read More about Efficacy of antimicrobial and anti-viral coated air filters to prevent the spread of airborne pathogens.

Correction to “Bespoke 3D-Printed Polydrug Implants Created via Microstructural Control of Oligomers” (2022)
Journal Article
Ruiz-Cantu, L., Trindade, G. F., Taresco, V., Zhou, Z., He, Y., Burroughs, L., Clark, E. A., Rose, F. R. A. J., Tuck, C., Hague, R., Roberts, C. J., Alexander, M., Irvine, D. J., & Wildman, R. D. (2022). Correction to “Bespoke 3D-Printed Polydrug Implants Created via Microstructural Control of Oligomers”. ACS Applied Materials and Interfaces, 14(6), 8654. https://doi.org/10.1021/acsami.2c00035

The chemical structure of the drug trandolapril has been corrected in Figure 4c. The conclusions of the work have not been affected by this correction. (Figure present).

Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content (2022)
Journal Article
Taylor, M., Simoes, F., Smith, J., Genapathy, S., Canning, A., Lledos, M., Chan, W. C., Denning, C., Scurr, D. J., Steven, R. T., Spencer, S. J., Shard, A. G., Alexander, M. R., & Zelzer, M. (2022). Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content. Surface and Interface Analysis, 54(4), 417-432. https://doi.org/10.1002/sia.7052

Peptide-coated surfaces are widely employed in biomaterial design, but quantifiable correlation between surface composition and biological response is challenging due to, for example, instrumental limitations, a lack of suitable model surfaces or lim... Read More about Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content.

Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices (2021)
Journal Article
He, Y., Luckett, J., Begines, B., Dubern, J. F., Hook, A. L., Prina, E., Rose, F. R., Tuck, C. J., Hague, R. J., Irvine, D. J., Williams, P., Alexander, M. R., & Wildman, R. D. (2022). Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices. Biomaterials, 281, Article 121350. https://doi.org/10.1016/j.biomaterials.2021.121350

Chronic infection as a result of bacterial biofilm formation on implanted medical devices is a major global healthcare problem requiring new biocompatible, biofilm-resistant materials. Here we demonstrate how bespoke devices can be manufactured throu... Read More about Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices.

Immune-instructive materials as new tools for immunotherapy (2021)
Journal Article
Fisher, L. E., Kämmerling, L., Alexander, M. R., & Ghaemmaghami, A. M. (2022). Immune-instructive materials as new tools for immunotherapy. Current Opinion in Biotechnology, 74, 194-203. https://doi.org/10.1016/j.copbio.2021.11.005

Immune instructive materials, are materials with the ability to modulate or mimic the function of immune cells, provide exciting opportunities for developing new therapies in many areas including medical devices, chronic inflammation, cancer, and aut... Read More about Immune-instructive materials as new tools for immunotherapy.

Customisable Tablet Printing: The Development of Multimaterial Hot Melt Inkjet 3D Printing to Produce Complex and Personalised Dosage Forms (2021)
Journal Article
Lion, A., Wildman, R. D., Alexander, M. R., & Roberts, C. J. (2021). Customisable Tablet Printing: The Development of Multimaterial Hot Melt Inkjet 3D Printing to Produce Complex and Personalised Dosage Forms. Pharmaceutics, 13(10), Article 1679. https://doi.org/10.3390/pharmaceutics13101679

One of the most striking characteristics of 3D printing is its capability to produce multi-material objects with complex geometry. In pharmaceutics this translates to the possibility of dosage forms with multi-drug loading, tailored dosing and releas... Read More about Customisable Tablet Printing: The Development of Multimaterial Hot Melt Inkjet 3D Printing to Produce Complex and Personalised Dosage Forms.

A new particle mounting method for surface analysis (2021)
Journal Article
Dundas, A. A., Kern, S., Cuzzucoli Crucitti, V., Scurr, D. J., Wildman, R., Irvine, D. J., & Alexander, M. R. (2022). A new particle mounting method for surface analysis. Surface and Interface Analysis, 54(4), 374-380. https://doi.org/10.1002/sia.7010

The chemical analysis of microparticles is challenging due to the need to mount the particles on a substrate for analysis; double-sided adhesive tape is often used (sometimes conductive), however that is usually coated with poly (dimethyl siloxane) (... Read More about A new particle mounting method for surface analysis.

Generation and Characterization of a Library of Novel Biologically Active Functional Surfactants (Surfmers) Using Combined High-Throughput Methods (2021)
Journal Article
Cuzzucoli Crucitti, V., Contreas, L., Taresco, V., Howard, S. C., Dundas, A. A., Limo, M. J., Nisisako, T., Williams, P. M., Williams, P., Alexander, M. R., Wildman, R. D., Muir, B. W., & Irvine, D. J. (2021). Generation and Characterization of a Library of Novel Biologically Active Functional Surfactants (Surfmers) Using Combined High-Throughput Methods. ACS Applied Materials and Interfaces, 13(36), 43290-43300. https://doi.org/10.1021/acsami.1c08662

We report the first successful combination of three distinct high-throughput techniques to deliver the accelerated design, synthesis, and property screening of a library of novel, bio-instructive, polymeric, comb-graft surfactants. These three-dimens... Read More about Generation and Characterization of a Library of Novel Biologically Active Functional Surfactants (Surfmers) Using Combined High-Throughput Methods.

Bespoke 3D-Printed Polydrug Implants Created via Microstructural Control of Oligomers (2021)
Journal Article
Ruiz-Cantu, L., Trindade, G. F., Taresco, V., Zhou, Z., He, Y., Burroughs, L., Clark, E. A., Rose, F. R., Tuck, C., Hague, R., Roberts, C. J., Alexander, M., Irvine, D. J., & Wildman, R. D. (2021). Bespoke 3D-Printed Polydrug Implants Created via Microstructural Control of Oligomers. ACS Applied Materials and Interfaces, 13(33), 38969-38978. https://doi.org/10.1021/acsami.1c07850

Controlling the microstructure of materials by means of phase separation is a versatile tool for optimizing material properties. Phase separation has been exploited to fabricate intricate microstructures in many fields including cell biology, tissue... Read More about Bespoke 3D-Printed Polydrug Implants Created via Microstructural Control of Oligomers.

Droplet Microfluidic Optimisation Using Micropipette Characterisation of Bio-Instructive Polymeric Surfactants (2021)
Journal Article
Henshaw, C. A., Dundas, A. A., Cuzzucoli Crucitti, V., Alexander, M. R., Wildman, R., Rose, F. R. A. J., Irvine, D. J., & Williams, P. M. (2021). Droplet Microfluidic Optimisation Using Micropipette Characterisation of Bio-Instructive Polymeric Surfactants. Molecules, 26(11), Article 3302. https://doi.org/10.3390/molecules26113302

Droplet microfluidics can produce highly tailored microparticles whilst retaining monodispersity. However, these systems often require lengthy optimisation, commonly based on a trial-and-error approach, particularly when using bio-instructive, polyme... Read More about Droplet Microfluidic Optimisation Using Micropipette Characterisation of Bio-Instructive Polymeric Surfactants.