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Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content

Taylor, Michael; Simoes, Fabio; Smith, James; Genapathy, Sivaneswary; Canning, Anne; Lledos, Marina; Chan, Weng C.; Denning, Chris; Scurr, David J.; Steven, Rory T.; Spencer, Steve J.; Shard, Alexander G.; Alexander, Morgan R.; Zelzer, Mischa

Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content Thumbnail


Authors

Michael Taylor

Fabio Simoes

James Smith

Sivaneswary Genapathy

Anne Canning

Marina Lledos

CHRIS DENNING chris.denning@nottingham.ac.uk
Professor of Stem Cell Biology

DAVID SCURR DAVID.SCURR@NOTTINGHAM.AC.UK
Principal Research Fellow

Rory T. Steven

Steve J. Spencer

Alexander G. Shard

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MORGAN ALEXANDER MORGAN.ALEXANDER@NOTTINGHAM.AC.UK
Professor of Biomedical Surfaces



Abstract

Peptide-coated surfaces are widely employed in biomaterial design, but quantifiable correlation between surface composition and biological response is challenging due to, for example, instrumental limitations, a lack of suitable model surfaces or limitations in quantitatively correlating data from different surface analytical techniques. Here, we first establish a reference material that allows control over amino acid content. Reversible addition-fragmentation chain-transfer (RAFT) polymerisation is used to prepare a copolymer containing alkyne and furan units with well-defined chain length and composition. Huisgen Cu(I)-catalysed azide-alkyne cycloaddition reaction is used to attach the model azido-polyethyleneglycol-amide-modified pentafluoro-l-phenylalanine to the polymer. Different compositional ratios of the polymer provide a surface with varying amino acid content that is analysed by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Nitrogen-related signals are compared with fluorine signals from both techniques. Fluorine and nitrogen signals from both techniques are found to be related to the copolymer compositions, but the homopolymer data deviate from this trend. The approach is then translated to a heparin-binding peptide that supports cell adhesion. Human embryonic stem cells cultured on copolymer surfaces presenting different amounts of heparin-binding peptide show strong cell growth while maintaining pluripotency after 72 h of culture. The early cell adhesion at 24 h can be correlated to the logarithm of the normalised CH4N+ ion intensity from ToF-SIMS data, which is established as a suitable and generalisable marker ion for amino acids and peptides. This work contributes to the ability to use ToF-SIMS in a more quantitative manner for the analysis of amino acid and peptide surfaces.

Citation

Taylor, M., Simoes, F., Smith, J., Genapathy, S., Canning, A., Lledos, M., …Zelzer, M. (2022). Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content. Surface and Interface Analysis, 54(4), 417-432. https://doi.org/10.1002/sia.7052

Journal Article Type Article
Acceptance Date Nov 29, 2021
Online Publication Date Jan 25, 2022
Publication Date 2022-04
Deposit Date Jan 26, 2022
Publicly Available Date Mar 29, 2024
Journal Surface and Interface Analysis
Print ISSN 0142-2421
Electronic ISSN 1096-9918
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 54
Issue 4
Pages 417-432
DOI https://doi.org/10.1002/sia.7052
Keywords Materials Chemistry; Surfaces, Coatings and Films; Surfaces and Interfaces; Condensed Matter Physics; General Chemistry
Public URL https://nottingham-repository.worktribe.com/output/7341520
Publisher URL https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/sia.7052

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