Professor MORGAN ALEXANDER's Outputs (142)

Modulation of the biological response to surfaces through the controlled deposition of 3D polymeric surfactants (2025)
Journal Article
Cuzzucoli Crucitti, V., Hajiali, H., Dundas, A. A., Jayawarna, V., Tomolillo, D., Francolini, I., Vuotto, C., Salmeron-Sanchez, M., Dalby, M. J., Alexander, M. R., Wildman, R. D., Rose, F. R. A. J., & Irvine, D. J. (2025). Modulation of the biological response to surfaces through the controlled deposition of 3D polymeric surfactants. Journal of Materials Chemistry B, 13(15), 4657-4670. https://doi.org/10.1039/d4tb01941e

Biomaterials play a crucial role in modern medicine through their use as medical implants and devices. However, they can support biofilm formation and infection, and lack integration with the surrounding human tissue at the implant site. This work re... Read More about Modulation of the biological response to surfaces through the controlled deposition of 3D polymeric surfactants.

Screening of modular supramolecular star polymers for 3D printing of biomedical devices (2025)
Journal Article
Hart, L. R., Touré, A. B., Owen, R., Putri, N. R., Hague, R. J., Alexander, M. R., Rose, F. R., Zhou, Z., Irvine, D. J., Ruiz-Cantu, L., Turyanska, L., He, Y., Hayes, W., & Wildman, R. D. (2025). Screening of modular supramolecular star polymers for 3D printing of biomedical devices. Materials Today Communications, 45, Article 112206. https://doi.org/10.1016/j.mtcomm.2025.112206

Identifying suitable materials for additive manufacturing and 3D printing is a challenging task and there is a need to streamline the processes to achieve more rapid adoption of new feedstocks. We have developed a process of using modular supramolecu... Read More about Screening of modular supramolecular star polymers for 3D printing of biomedical devices.

Molecular orientation and stratification revealed in RNA-lipid nanoparticles using Cryogenic Orbitrap Secondary Ion Mass Spectrometry (Cryo-OrbiSIMS) depth profiling (2025)
Preprint / Working Paper
Kotowska, A., Fay, M., Watts, J., Gilmore, I., Scurr, D., Howe, A., Capka, V., Perez, C., Doud, D., Patel, S., Umbarger, M., Langer, R., & Alexander, M. (2025). Molecular orientation and stratification revealed in RNA-lipid nanoparticles using Cryogenic Orbitrap Secondary Ion Mass Spectrometry (Cryo-OrbiSIMS) depth profiling

Lipid nanoparticle RNA (LNP-RNA) formulations are used for the delivery of vaccines and other therapies. RNA molecules are encapsulated within their interior through electrostatic interactions with positively charged lipids. The identity of the lipid... Read More about Molecular orientation and stratification revealed in RNA-lipid nanoparticles using Cryogenic Orbitrap Secondary Ion Mass Spectrometry (Cryo-OrbiSIMS) depth profiling.

Surface Lipids in Nematodes are Influenced by Development and Species-specific Adaptations (2025)
Journal Article
Kotowska, A. M., Hiramatsu, F., Alexander, M. R., Scurr, D. J., Lightfoot, J. W., & Chauhan, V. M. (2025). Surface Lipids in Nematodes are Influenced by Development and Species-specific Adaptations. Journal of the American Chemical Society, 147(8), 6439-6449. https://doi.org/10.1021/jacs.4c12519

The surface of an organism is a dynamic interface that continually adapts to its environment. In nematodes, the cuticle forms a complex boundary that protects against the physicochemical pressures. However, the precise molecular composition and funct... Read More about Surface Lipids in Nematodes are Influenced by Development and Species-specific Adaptations.

Development of C. albican Anti-attachment Inkjet 3D Printing Ink, via High Throughput Screening (2025)
Presentation / Conference Contribution
Yong, L. X., Zhou, Z., Vallières, C., He, Y., Cuzzucoli Crucitti, V., Alexander, M. R., Avery, S., Wildman, R., & Irvine, D. (2024, August). Development of C. albican Anti-attachment Inkjet 3D Printing Ink, via High Throughput Screening. Presented at International Conference on Computational & Experimental Engineering and Sciences, Singapore, Singapore

The fungal pathogen Candida albicans (C. albicans) is particularly problematic for immunocompromised patients and those with medical implants. Introducing Candida-resistant medical devices could potentially reduce mortality rates from such infections... Read More about Development of C. albican Anti-attachment Inkjet 3D Printing Ink, via High Throughput Screening.

Monosaccharide coating modulate the intracellular trafficking of gold nanoparticles in dendritic cells (2024)
Journal Article
Alobaid, M. A., Richards, S.-J., Alexander, M. R., Gibson, M. I., & Ghaemmaghami, A. M. (2024). Monosaccharide coating modulate the intracellular trafficking of gold nanoparticles in dendritic cells. Materials Today Bio, 29, Article 101371. https://doi.org/10.1016/j.mtbio.2024.101371

Dendritic cells (DCs) have emerged as a promising target for drug delivery and immune modulation due to their pivotal role in initiating the adaptive immune response. Gold nanoparticles (AuNPs) have garnered interest as a platform for targeted drug d... Read More about Monosaccharide coating modulate the intracellular trafficking of gold nanoparticles in dendritic cells.

Fungal Attachment-Resistant Polymers for the Additive Manufacture of Medical Devices (2024)
Journal Article
Yong, L. X., Sefton, J., Vallières, C., Rance, G. A., Hill, J., Cuzzucoli Crucitti, V., Dundas, A. A., Rose, F. R., Alexander, M. R., Wildman, R., He, Y., Avery, S. V., & Irvine, D. J. (2024). Fungal Attachment-Resistant Polymers for the Additive Manufacture of Medical Devices. ACS Applied Materials and Interfaces, 16(40), 54508–54519. https://doi.org/10.1021/acsami.4c04833

This study reports the development of the first copolymer material that (i) is resistant to fungal attachment and hence biofilm formation, (ii) operates via a nonkilling mechanism, i.e., avoids the use of antifungal actives and the emergence of funga... Read More about Fungal Attachment-Resistant Polymers for the Additive Manufacture of Medical Devices.

Discovery and Computational Modelling of Adsorbent Polymers that Effectively Immobilize SARS-CoV-2 with Potential Practical Applications (2024)
Journal Article
Xue, X., Duncan, J. D., Coleman, C. M., Contreas, L., Blackburn, C., Vivero-Lopez, M., Williams, P. M., Ball, J. K., Alexander, C., & Alexander, M. R. (2024). Discovery and Computational Modelling of Adsorbent Polymers that Effectively Immobilize SARS-CoV-2 with Potential Practical Applications. Cell Reports Physical Science, 5(9), Article 102204. https://doi.org/10.1016/j.xcrp.2024.102204

Viral translocation is considered a common way for respiratory viruses to spread and contaminate the surrounding environment. Thus, the discovery of non-eluting polymers that immobilize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) upo... Read More about Discovery and Computational Modelling of Adsorbent Polymers that Effectively Immobilize SARS-CoV-2 with Potential Practical Applications.

Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents (2024)
Journal Article
Brookes, A., Kindon, N., Scurr, D., Alexander, M., Gershkovich, P., & BRADSHAW, T. (2024). Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents. British Journal of Cancer, 2, Article 67. https://doi.org/10.1038/s44276-024-00088-0

Background
Glioblastoma multiforme (GBM) is an aggressive cancer with poor prognosis, partly due to resistance to the standard chemotherapy treatment, temozolomide (TMZ). Phytocannabinoid cannabidiol (CBD) has exhibited anti-cancer effects against G... Read More about Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents.

Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents (2024)
Journal Article
Brookes, A., Kindon, N., Scurr, D. J., Alexander, M. R., Gershkovich, P., & Bradshaw, T. (2024). Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents. BJC Reports, 2, Article 67. https://doi.org/10.1038/s44276-024-00088-0

Background
Glioblastoma multiforme (GBM) is an aggressive cancer with poor prognosis, partly due to resistance to the standard chemotherapy treatment, temozolomide (TMZ). Phytocannabinoid cannabidiol (CBD) has exhibited anti-cancer effects against G... Read More about Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents.

Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy (2024)
Journal Article
Owen, R., Nasir, A., H. Amer, M., Nie, C., Xue, X., Burroughs, L., Denning, C., D. Wildman, R., A. Khan, F., R. Alexander, M., & R. A. J. Rose, F. (2024). Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy. Advanced Intelligent Systems, Article 2400573. https://doi.org/10.1002/aisy.202400573

High-throughput screening (HTS) can be used when ab initio information is unavailable for rational design of new materials, generating data on properties such as chemistry and topography that control cell behavior. Biomaterial screens are typically f... Read More about Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy.

Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS (2024)
Journal Article
Lu, L., Kotowska, A. M., Kern, S., Fang, M., Rudd, T. R., Alexander, M. R., Scurr, D. J., & Zhu, Z. (2024). Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS. Analytical Chemistry, https://doi.org/10.1021/acs.analchem.4c01201

Growing clinical evidence reveals that systematic molecular alterations in the brain occur 20 years before the onset of AD pathological features. Apolipoprotein E4 (ApoE4) is one of the most significant genetic risk factors for Alzheimer’s disease (A... Read More about Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS.

Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids (2024)
Journal Article
Suvannapruk, W., Fisher, L. E., Luckett, J. C., Edney, M. K., Kotowska, A. M., Kim, D. H., Scurr, D. J., Ghaemmaghami, A. M., & Alexander, M. R. (2024). Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids. Advanced Science, 11(15), Article 2306000. https://doi.org/10.1002/advs.202306000

A key goal for implanted medical devices is that they do not elicit a detrimental immune response. Macrophages play critical roles in the modulation of the host immune response and are the cells responsible for persistent inflammatory reactions to im... Read More about Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids.

Polymer-Coated Urinary Catheter Reduces Biofilm Formation and Biomineralization: A First-in-Man, Prospective Pilot Study (2024)
Journal Article
Kalenderski, K., Dubern, J.-F., Lewis-Lloyd, C., Jeffery, N., Heeb, S., Irvine, D. J., Sloan, T. J., Birch, B., Andrich, D., Humes, D., Alexander, M. R., & Williams, P. (2024). Polymer-Coated Urinary Catheter Reduces Biofilm Formation and Biomineralization: A First-in-Man, Prospective Pilot Study. Journal of Urology Open PLus, 2(1), Article e00005. https://doi.org/10.1097/JU9.0000000000000097

Purpose: Biofilm formation and biomineralization on urinary catheters may cause severe complications including infection and obstruction. Here, we describe an in vitro evaluation and prospective pilot clinical study of a silicone catheter coated with... Read More about Polymer-Coated Urinary Catheter Reduces Biofilm Formation and Biomineralization: A First-in-Man, Prospective Pilot Study.

Investigating ApoE4‐mediated metabolic alteration of H4 neuroglioma cells of Alzheimer’s disease (2023)
Presentation / Conference Contribution
Lu, L., Kotowska, A., Kern, S., Fang, M., Rudd, T. R., Alexander, M., Scurr, D., & Zhu, Z. Investigating ApoE4‐mediated metabolic alteration of H4 neuroglioma cells of Alzheimer’s disease. Presented at Alzheimer’s Association International Conference 2023, Amterdam, Netherlands and online

Background
Metabolites and proteins in ApoE4‐carried H4 neuroglioma cells were analyzed to investigate the potential mechanism of the risk gene ApoE4 involved in Alzheimer’s disease (AD).

Method
Metabolomics of ApoE4‐carried and wild‐type H4 cel... Read More about Investigating ApoE4‐mediated metabolic alteration of H4 neuroglioma cells of Alzheimer’s disease.

Toward Comprehensive Analysis of the 3D Chemistry of Pseudomonas aeruginosa Biofilms (2023)
Journal Article
Kotowska, A. M., Zhang, J., Carabelli, A., Watts, J., Aylott, J. W., Gilmore, I. S., Williams, P., Scurr, D. J., & Alexander, M. R. (2023). Toward Comprehensive Analysis of the 3D Chemistry of Pseudomonas aeruginosa Biofilms. Analytical Chemistry, 95(49), 18287-18294. https://doi.org/10.1021/acs.analchem.3c04443

Bacterial biofilms are structured communities consisting of cells enmeshed in a self-generated extracellular matrix usually attached to a surface. They contain diverse classes of molecules including polysaccharides, lipids, proteins, nucleic acids, a... Read More about Toward Comprehensive Analysis of the 3D Chemistry of Pseudomonas aeruginosa Biofilms.

Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model (2023)
Journal Article
Dooley, M., Luckett, J., Alexander, M. R., Matousek, P., Dehghani, H., Ghaemmaghami, A. M., & Notingher, I. (2023). Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model. Biomedical Optics Express, 14(12), 6592-6606. https://doi.org/10.1364/boe.512118

Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modeling of light propagation was used as a method for improving the design of an DRS instrument to maximize the signal to noise ratio (SNR)... Read More about Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model.

Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model (2023)
Journal Article
Dooley, M., Luckett, J., Alexander, M. R., Matousek, P., Dehghani, H., Ghaemmaghami, A. M., & Notingher, I. (2023). Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model. Biomedical Optics Express, 14(12), 6592-6606. https://doi.org/10.1364/BOE.512118

Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modelling of light propagation was used as a method for improving the design of an DRS instrument to maximize the signal to noise ratio (SNR)... Read More about Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model.

A potential alternative to fungicides using actives-free (meth)acrylate polymers for protection of wheat crops from fungal attachment and infection (2023)
Journal Article
Crawford, L. A., Cuzzucoli Crucitti, V., Stimpson, A., Morgan, C., Blake, J., Wildman, R. D., Hook, A. L., Alexander, M. R., Irvine, D. J., & Avery, S. V. (2023). A potential alternative to fungicides using actives-free (meth)acrylate polymers for protection of wheat crops from fungal attachment and infection. Green Chemistry, 25(21), 8558-8569. https://doi.org/10.1039/d3gc01911j

Fungicidal compounds are actives widely used for crop protection from fungal infection, but they can also kill beneficial organisms, enter the food chain and promote resistant pathogen strains from overuse. Here we report the first field crop trial o... Read More about A potential alternative to fungicides using actives-free (meth)acrylate polymers for protection of wheat crops from fungal attachment and infection.

Label-free Chemical Characterization of Polarized Immune Cells in vitro and Host Response to Implanted Bio-instructive Polymers in vivo Using 3D OrbiSIMS (2023)
Journal Article
Suvannapruk, W., Edney, M. K., Fisher, L. E., Luckett, J. C., Kim, D.-H., Scurr, D. J., Ghaemmaghami, A. M., & Alexander, M. R. (2023). Label-free Chemical Characterization of Polarized Immune Cells in vitro and Host Response to Implanted Bio-instructive Polymers in vivo Using 3D OrbiSIMS. Bio-protocol, 13(15), Article e4727. https://doi.org/10.21769/BioProtoc.4727

The Three-dimensional OrbiTrap Secondary Ion Mass Spectrometry (3D OrbiSIMS) is a secondary ion mass spectrometry instrument, a combination of a Time of Flight (ToF) instrument with an Orbitrap analyzer. The 3D OrbiSIMS technique is a powerful tool f... Read More about Label-free Chemical Characterization of Polarized Immune Cells in vitro and Host Response to Implanted Bio-instructive Polymers in vivo Using 3D OrbiSIMS.