Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids

Suvannapruk, Waraporn; Fisher, Leanne E.; Luckett, Jeni C.; Edney, Max K.; Kotowska, Anna M.; Kim, Dong Hyun; Scurr, David J.; Ghaemmaghami, Amir M.; Alexander, Morgan R.

Abstract

A key goal for implanted medical devices is that they do not elicit a detrimental immune response. Macrophages play critical roles in the modulation of the host immune response and are the cells responsible for persistent inflammatory reactions to implanted biomaterials. Two novel immune-instructive polymers that stimulate pro- or anti-inflammatory responses from macrophages in vitro are investigated. These also modulate in vivo foreign body responses (FBR) when implanted subcutaneously in mice. Immunofluorescent staining of tissue abutting the polymer reveals responses consistent with pro- or anti-inflammatory responses previously described for these polymers. Three Dimensional OrbiTrap Secondary Ion Mass Spectrometry (3D OrbiSIMS) analysis to spatially characterize the metabolites in the tissue surrounding the implant, providing molecular histology insight into the metabolite response in the host is applied. For the pro-inflammatory polymer, monoacylglycerols (MG) and diacylglycerols (DG) are observed at increased intensity, while for the anti-inflammatory coating, the number of phospholipid species detected decreased, and pyridine and pyrimidine levels are elevated. Small molecule signatures from single-cell studies of M2 macrophages in vitro correlate with the in vivo observations, suggesting potential for prediction. Metabolite characterization by the 3D OrbiSIMS is shown to provide insight into the mechanism of bio-instructive materials as medical devices and to inform on the FBR to biomaterials.

Citation

Suvannapruk, W., Fisher, L. E., Luckett, J. C., Edney, M. K., Kotowska, A. M., Kim, D. H., Scurr, D. J., Ghaemmaghami, A. M., & Alexander, M. R. (2024). Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids. Advanced Science, 11(15), Article 2306000. https://doi.org/10.1002/advs.202306000

• Journal Article Type: Article
• Acceptance Date: Jan 19, 2024
• Online Publication Date: Feb 14, 2024
• Publication Date: Apr 17, 2024
• Deposit Date: Dec 20, 2023
• Publicly Available Date: Jan 19, 2024
• Journal: Advanced Science
• Electronic ISSN: 2198-3844
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 15
• Article Number: 2306000
• DOI: https://doi.org/10.1002/advs.202306000
• Keywords: Macrophage, 3D OrbiSIMS, immune-instructive polymers and metabolomics
• Public URL: https://nottingham-repository.worktribe.com/output/24585971
• Publisher URL: https://onlinelibrary.wiley.com/journal/21983844